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    iscR DNA-binding transcriptional dual regulator IscR [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945279, updated on 3-Dec-2024

    Summary

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    Official Symbol
    iscR
    Official Full Name
    DNA-binding transcriptional dual regulator IscR
    Primary source
    ECOCYC:G7326
    Locus tag
    b2531
    See related
    ASAP:ABE-0008326
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2528; yfhP
    Summary
    IscR is a member of the Mar/Sox/Rob family of transcriptional regulators. [More information is available at EcoCyc: G7326].
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    Genomic context

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    Sequence:
    NC_000913.3 (2661643..2662131, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene scaffold protein for iron-sulfur cluster assembly Neighboring gene cysteine desulfurase IscS Neighboring gene tRNA Cm32/Um32 methyltransferase Neighboring gene Nus factor SuhB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Coordinate regulation of the Suf and Isc Fe-S cluster biogenesis pathways by IscR is essential for viability of Escherichia coli. Mettert EL, et al. J Bacteriol, 2014 Dec. PMID 25266384, Free PMC Article
    2. Minor Alterations in Core Promoter Element Positioning Reveal Functional Plasticity of a Bacterial Transcription Factor. Chowdhury WP, et al. mBio, 2021 Dec 21. PMID 34724814, Free PMC Article
    3. Regulation of iron-sulphur cluster homeostasis through transcriptional control of the Isc pathway by [2Fe-2S]-IscR in Escherichia coli. Giel JL, et al. Mol Microbiol, 2013 Feb. PMID 23075318, Free PMC Article
    4. A Regulatory Circuit Composed of a Transcription Factor, IscR, and a Regulatory RNA, RyhB, Controls Fe-S Cluster Delivery. Mandin P, et al. mBio, 2016 Sep 20. PMID 27651365, Free PMC Article
    5. Feedback regulation of iron-sulfur cluster biosynthesis. Frazzon J, et al. Proc Natl Acad Sci U S A, 2001 Dec 18. PMID 11752417, Free PMC Article

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Minor Alterations in Core Promoter Element Positioning Reveal Functional Plasticity of a Bacterial Transcription Factor.
      Title: Minor Alterations in Core Promoter Element Positioning Reveal Functional Plasticity of a Bacterial Transcription Factor.
    2. findings show how an sRNA, RyhB, and a transcription factor, IscR, regulate expression of an essential gene, erpA, in E. coli
      Title: A Regulatory Circuit Composed of a Transcription Factor, IscR, and a Regulatory RNA, RyhB, Controls Fe-S Cluster Delivery.
    3. Authors demonstrate that in the absence of isc, IscR-dependent suf activation is essential since strains lacking both the Isc pathway and IscR were not viable unless Suf was expressed ectopically.
      Title: Coordinate regulation of the Suf and Isc Fe-S cluster biogenesis pathways by IscR is essential for viability of Escherichia coli.
    4. These data suggest a unique mechanism of ligand-mediated DNA site recognition, whereby metallocluster ligation relocates a protein-specificity determinant to expand DNA target-site selection, allowing a broader transcriptomic response by holo-IscR.
      Title: Studies of IscR reveal a unique mechanism for metal-dependent regulation of DNA binding specificity.
    5. Fe-S clusters directed at the apo-NsrR and apo-IscR proteins are built on either of the two scaffolds, IscU or SufB.
      Title: In vivo [Fe-S] cluster acquisition by IscR and NsrR, two stress regulators in Escherichia coli.
    6. Together, these data show how transcriptional control of iscRSUA-hscBA-fdx by [2Fe-2S]-IscR allows E. coli to respond efficiently to varying Fe-S demands.
      Title: Regulation of iron-sulphur cluster homeostasis through transcriptional control of the Isc pathway by [2Fe-2S]-IscR in Escherichia coli.
    7. a combination of ArcA and AppY antirepression of IscR function, lower levels of IscR, and weak repression by [2Fe-2S]-IscR leads to increased hyaA promoter expression under anaerobic conditions
      Title: ArcA and AppY antagonize IscR repression of hydrogenase-1 expression under anaerobic conditions, revealing a novel mode of O2 regulation of gene expression in Escherichia coli.
    8. These results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort.
      Title: Double locking of an Escherichia coli promoter by two repressors prevents premature colicin expression and cell lysis.
    9. Activation of the suf operon by IscR occurs only under conditions where Fur loses its repressor activity.
      Title: Oxidant-responsive induction of the suf operon, encoding a Fe-S assembly system, through Fur and IscR in Escherichia coli.
    10. The findings presented here indicate a more general role of IscR in the regulation of Fe-S cluster biogenesis and that IscR contributes to the O(2) regulation of several promoters controlling the expression of anaerobic Fe-S proteins.
      Title: IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O2-regulated genes in Escherichia coli.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2 iron, 2 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator IscR

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2661643..2662131 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417026.1 DNA-binding transcriptional dual regulator IscR [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417026.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5C9A9Z8
      Conserved Domains (1) summary
      PRK10857
      Location:1 → 162
      PRK10857; Fe-S cluster assembly transcriptional regulator IscR
    Nucleotide Protein
    Heading Accession and Version

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