slmA nucleoid occlusion factor SlmA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: slmA
Official Full Name: nucleoid occlusion factor SlmA
Primary source: ECOCYC:EG11191
Locus tag: b3641
See related: ASAP:ABE-0011902
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3631; ttk; yicB
Summary: TetR family. [More information is available at EcoGene: EG11191]. SlmA is a cell division inhibitor that is required for nucleoid exclusion . slmA null mutants are viable and essentially indistinguishable from wild-type cells , but a slmA min double mutant has a synthetic lethal phenotype . [More information is available at EcoCyc: EG11191].
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Genomic context

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Sequence:: NC_000913.3 (3814494..3815090)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lipid Surfaces and Glutamate Anions Enhance Formation of Dynamic Biomolecular Condensates Containing Bacterial Cell Division Protein FtsZ and Its DNA-Bound Regulator SlmA.
Title: Lipid Surfaces and Glutamate Anions Enhance Formation of Dynamic Biomolecular Condensates Containing Bacterial Cell Division Protein FtsZ and Its DNA-Bound Regulator SlmA.
The Nucleoid Occlusion Protein SlmA Binds to Lipid Membranes.
Title: The Nucleoid Occlusion Protein SlmA Binds to Lipid Membranes.
Here, we demonstrate that FtsZ can form crowding-induced condensates, reminiscent of those observed for eukaryotic proteins. Formation of these FtsZ-rich droplets occurs when FtsZ is bound to SlmA, a spatial regulator of FtsZ that antagonizes polymerization, while also binding to specific sites on chromosomal DNA.
Title: Bacterial FtsZ protein forms phase-separated condensates with its nucleoid-associated inhibitor SlmA.
Combined, these data reveal the molecular basis for the SlmA-DNA-FtsZ interaction with implications for SlmA's NO function and underscore the ability of the FtsZ CTD to adopt a wide range of conformations, explaining its ability to bind diverse regulatory proteins.
Title: Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.
even in the absence of Min, SlmA, and the putative Ter macrodomain - Z-ring link, there remains a weak midcell positioning bias for the Z-ring.
Title: Evidence for divisome localization mechanisms independent of the Min system and SlmA in Escherichia coli.
these results lead to a model in which SlmA binding to an SBS is activated to bind the tail of FtsZ resulting in further interaction with FtsZ leading to depolymerization of FtsZ polymers.
Title: SlmA antagonism of FtsZ assembly employs a two-pronged mechanism like MinCD.
Structures show that SlmA dramatically distorts DNA, allowing it to bind as an orientated dimer-of-dimers. Biochemical data indicate that SlmA dimer-of-dimers can spread along the DNA.
Title: SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
In this case, the adaptation ensures that SlmA only blocks cytokinesis in regions of the cell occupied by the origin-proximal portion of the chromosome where SlmA-binding sites are enriched
Title: Identification of the SlmA active site responsible for blocking bacterial cytokinetic ring assembly over the chromosome.
SlmA binds DNA and FtsZ simultaneously, and the SlmA-FtsZ structure reveals that two FtsZ molecules sandwich a SlmA dimer.
Title: Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check.
Data suggest that SlmA is a DNA-associated division inhibitor that is directly involved in preventing Z ring assembly on portions of the membrane surrounding the nucleoid [SlmA]
Title: SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within division septum site selection	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of division septum assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of division septum assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of division septum assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of protein polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell division	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in bacterial nucleoid	IDA Inferred from Direct Assay more info	PubMed
located_in bacterial nucleoid	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoid	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: nucleoid occlusion factor SlmA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3814494..3815090

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418098.4 nucleoid occlusion factor SlmA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418098.4

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376FSC2, A0A5B9ATN7

Conserved Domains (1) summary

PRK09480
Location:1 → 195

slmA; division inhibitor protein; Provisional