EST1 Est1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EST1
Official Full Name: Est1p
Primary source: SGD:S000004223
Locus tag: YLR233C
See related: AllianceGenome:SGD:S000004223; FungiDB:YLR233C; VEuPathDB:YLR233C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA binding activity; single-stranded DNA binding activity; and telomeric DNA binding activity. Involved in G-quadruplex DNA formation; regulation of telomere maintenance via telomerase; and telomere maintenance via telomerase. Located in nucleolus. Part of telomerase holoenzyme complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See EST1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (607425..609524, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Est1 binds two adjacent, separate patches of Cdc13 with distinct pockets. The N-terminal interface of Cdc13-Est1 backstops TLC1-Ku-Sir4 to recruit telomerase. The C-terminal interface of Cdc13-Est1 is crucial for telomere maintenance in vivo.
Title: Structural Insights into Yeast Telomerase Recruitment to Telomeres.
Recruitment of Est1 to telomeres via a Cdc13-Est1 fusion is sufficient to bypass the role of Ku in promoting telomere elongation.
Title: The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.
Autonomous localization of Est1p to the nucleus via the classical nuclear import pathway contributes to normal telomere length maintenance.
Title: Normal telomere length maintenance in Saccharomyces cerevisiae requires nuclear import of the ever shorter telomeres 1 (Est1) protein via the importin alpha pathway.
Esl1 has no telomere-related functions, but contributes to the regulation of adaptive gene expression responses.
Title: Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses.
It is possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo.
Title: Characterization of the intramolecular G-quadruplex promoting activity of Est1.
There is a biochemical interaction between Est1 and the telomere binding protein Cdc13. Association of the Est1 protein with the telomerase catalytic core is not a regulatory event that contributes to telomere homeostasis.
Title: A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle.
the telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro
Title: The telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro.
The tricotetrapeptide repeat of Est1 is required for Est2-mediated telomere length maintenance.
Title: The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis.
These results demonstrate that one major functional role that Est1 brings to the telomerase complex is the capping or protection of telomeres.
Title: Est1 protects telomeres and inhibits subtelomeric y'-element recombination.
The authors show that the yeast telomerase subunit Est1p, known to be involved in telomerase recruitment to telomeres, can convert single-stranded telomeric G-rich DNA into a G-quadruplex structure in vitro in a Mg(2+)-dependent manner.
Title: Yeast telomerase subunit Est1p has guanine quadruplex-promoting activity that is required for telomere elongation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomerase RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G-quadruplex DNA formation	IDA Inferred from Direct Assay more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of telomere maintenance via telomerase	IDA Inferred from Direct Assay more info	PubMed
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via telomerase	IDA Inferred from Direct Assay more info	PubMed
involved_in telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of telomerase holoenzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of telomerase holoenzyme complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Est1p

NP_013334.1

TLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

607425..609524 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182120.1 → NP_013334.1 TPA: Est1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013334.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYN3, P17214, Q05997

UniProtKB/TrEMBL

B3RHC0, C7GR01, C8ZDJ7, N1P6W9

Conserved Domains (2) summary

pfam10373
Location:224 → 508

EST1_DNA_bind; Est1 DNA/RNA binding domain

pfam10374
Location:82 → 209

EST1; Telomerase activating protein Est1