SSD1 mRNA-binding translational repressor SSD1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SSD1
Official Full Name: mRNA-binding translational repressor SSD1
Primary source: SGD:S000002701
Locus tag: YDR293C
See related: AllianceGenome:SGD:S000002701; FungiDB:YDR293C; VEuPathDB:YDR293C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CLA1; MCS1; RLT1; SRK1
Summary: Enables mRNA 3'-UTR binding activity; mRNA 5'-UTR binding activity; and mRNA regulatory element binding translation repressor activity. Involved in several processes, including negative regulation of translation; regulation of G1 to G0 transition; and regulation of fungal-type cell wall organization. Located in cellular bud neck; cytoplasmic ribonucleoprotein granule; and nucleus. Human ortholog(s) of this gene implicated in Perlman syndrome and nephroblastoma. Orthologous to human DIS3L2 (DIS3 like 3'-5' exoribonuclease 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SSD1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1045640..1049392, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast Ssd1 is a non-enzymatic member of the RNase II family with an alternative RNA recognition site.
Title: Yeast Ssd1 is a non-enzymatic member of the RNase II family with an alternative RNA recognition site.
Interplay of the RNA Exosome Complex and RNA-Binding Protein Ssd1 in Maintaining Cell Wall Stability in Yeast.
Title: Interplay of the RNA Exosome Complex and RNA-Binding Protein Ssd1 in Maintaining Cell Wall Stability in Yeast.
Role of SSD1 in Phenotypic Variation of Saccharomyces cerevisiae Strains Lacking DEG1-Dependent Pseudouridylation.
Title: Role of SSD1 in Phenotypic Variation of Saccharomyces cerevisiae Strains Lacking DEG1-Dependent Pseudouridylation.
The genetic basis of aneuploidy tolerance in wild yeast.
Title: The genetic basis of aneuploidy tolerance in wild yeast.
Introduction of SSD1 to W303 diploids switches fate, in that it rescues cellular quiescence and disrupts the ability to sporulate. Ssd1 and another RNA-binding protein, Mpt5 (Puf5), have parallel roles in quiescence in haploids.
Title: Ssd1 and the cell wall integrity pathway promote entry, maintenance, and recovery from quiescence in budding yeast.
Ssd1 overexpression in young cells reduced translation and extended lifespan, while loss of Ssd1 reduced the translational deficit of old cells and shortened lifespan. Secondly, phosphorylation of eIF2alpha, mediated by the stress kinase Gcn2, was elevated in old cells, contributing to the global reduction in translation without detectable induction of the downstream Gcn4 transcriptional activator
Title: Ssd1 and Gcn2 suppress global translation efficiency in replicatively aged yeast while their activation extends lifespan.
Ssd1 regulates translation of its target message primarily through UTRs and the SIM1 message through multiple potential points of interaction, permitting fine translational control in various contexts.
Title: Cell morphogenesis proteins are translationally controlled through UTRs by the Ndr/LATS target Ssd1.
Both SSD1 and PDE2 seemed to improve ethanol tolerance by maintaining robustness of the yeast cell wall.
Title: Overexpression of PDE2 or SSD1-V in Saccharomyces cerevisiae W303-1A strain renders it ethanol-tolerant.
There is an Ssd1p-dependent cross-talk between chromosomal positioning within the nucleus and the positioning of cellular components within the cell in Saccharomyces cerevisiae.
Title: The relationship between chromosomal positioning within the nucleus and the SSD1 gene in Saccharomyces cerevisiae.
Ssd1p expressed from SSD1-V, but not from ssd1-d, stabilizes a subpopulation of CLN2 mRNA in wild-type and taf1-N568Delta strains and facilitates the continuous transcription of CLN2 after heat shock in the taf1-N568Delta strain.
Title: Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the 5'-untranslated region of CLN2 mRNA.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5'-RNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 5'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA regulatory element binding translation repressor activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in intracellular mRNA localization	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of G1 to G0 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of fungal-type cell wall organization	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in P-body	IBA Inferred from Biological aspect of Ancestor more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mRNA-binding translational repressor SSD1

NP_010579.1

Translational repressor; involved in polar growth, wall integrity, autodiploidization; regulated by Cbk1p phosphorylation to affect bud-specific translational control and localization of specific mRNAs; binds 5'UTRs of mRNAs encoding cell wall proteins; recognizes CNYTCNYT motif in RNA; interacts with TOR pathway components; nucleocytoplasmic shuttling appears to be critical to Ssd1p function; W303 lacks a functional SSD1 gene product; pseudonuclease descended from Dis3L2-family nucleases

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1045640..1049392 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180601.1 → NP_010579.1 TPA: mRNA-binding translational repressor SSD1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010579.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSS2, P24276

UniProtKB/TrEMBL

A6ZYP1, B3LG02, B5VGH0, C7GQU3, N1P8K2

Conserved Domains (1) summary

COG0557
Location:505 → 1149

VacB; Exoribonuclease R [Transcription]