MCM10 Mcm10p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MCM10
Official Full Name: Mcm10p
Primary source: SGD:S000001412
Locus tag: YIL150C
See related: AllianceGenome:SGD:S000001412; FungiDB:YIL150C; VEuPathDB:YIL150C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DNA43
Summary: Enables DNA replication origin binding activity and single-stranded DNA binding activity. Involved in DNA metabolic process and constitutive heterochromatin formation. Acts upstream of or within mitotic DNA replication DNA duplex unwinding. Located in nucleus and replication fork. Part of replication fork protection complex. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to human MCM10 (minichromosome maintenance 10 replication initiation factor). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MCM10 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (61013..62728, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

potent DNA strand-annealing protein that inhibits fork regression by SMARCAL1
Title: Mcm10 has potent strand-annealing activity and limits translocase-mediated fork regression.
This report demonstrates that Mcm10, an essential replication protein unique to eukaryotes, binds the replicative helicase CMG and greatly stimulates its helicase activity in vitro. Most significantly, Mcm10 enables CMG and the replisome to bypass blocks on the non-tracking DNA strand.
Title: Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks.
An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7.
Title: An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.
These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication.
Title: An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation.
Mcm10 directly binds the Minichromosome Maintenance Complex via Mcm2 and the Mcm6 C terminus.
Title: Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.
Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.
Title: Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.
In the absence of Mcm10, a stable Cdc45-Mcm-GINS (CMG) complex was assembled at origins. However subsequent translocation of CMG, replication protein A loading to origins, and the intra-S checkpoint activation were severely diminished.
Title: Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS.
These data indicate that Ctf4p facilitates Mcm10p to promote the DNA replication.
Title: Ctf4p facilitates Mcm10p to promote DNA replication in budding yeast.
Mcm10 plays two important roles as a linker of the MCM helicase and Polalpha at the elongating replication fork--first, to coordinate the activities of these two molecular motors
Title: Alternative mechanisms for coordinating polymerase alpha and MCM helicase.
analysis of DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae
Title: Novel DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic DNA replication DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in nuclear replication fork	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IMP Inferred from Mutant Phenotype more info	PubMed
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Mcm10p

NP_012116.1

Essential chromatin-associated protein; involved in initiation of DNA replication; promotes origin duplex unwinding by head-to-head CMG helicase complexes; required for association of MCM2-7 complex with replication origins; required to stabilize the catalytic subunit of DNA polymerase-alpha; coordinates function of the replication fork helicase; self-associates through its N-terminal domain