NHA1 Nha1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NHA1
Official Full Name: Nha1p
Primary source: SGD:S000004128
Locus tag: YLR138W
See related: AllianceGenome:SGD:S000004128; FungiDB:YLR138W; VEuPathDB:YLR138W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables sodium:proton antiporter activity. Involved in export across plasma membrane; intracellular potassium ion homeostasis; and response to osmotic stress. Located in membrane raft and plasma membrane. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NHA1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (418437..421394)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

C5 conserved region of hydrophilic C-terminal part of Saccharomyces cerevisiae Nha1 antiporter determines its requirement of Erv14 COPII cargo receptor for plasma-membrane targeting.
Title: C5 conserved region of hydrophilic C-terminal part of Saccharomyces cerevisiae Nha1 antiporter determines its requirement of Erv14 COPII cargo receptor for plasma-membrane targeting.
The 14-3-3 binding induces a disorder-to-order transition of the C-terminus of Nha1, and in vivo experiments showed that the mutation of Ser481 to Ala significantly increases cation efflux activity via Nha1, which renders cells sensitive to low K(+) concentrations.
Title: The activity of Saccharomyces cerevisiae Na(+), K(+)/H(+) antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481.
Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter.
Title: Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter.
All three potassium-exporting systems the Nha1 antiporter, Ena ATPase and Tok1 channel contribute to potassium homeostasis and are active upon potassium limitation in wild-type cells.
Title: Plasma-membrane hyperpolarization diminishes the cation efflux via Nha1 antiporter and Ena ATPase under potassium-limiting conditions.
sphingolipids are required for the stable localization of Nha1p to the plasma membrane
Title: Saccharomyces cerevisiae Na+/H+ antiporter Nha1p associates with lipid rafts and requires sphingolipid for stable localization to the plasma membrane.
results support the notion that Nha1p exists in membranes as a dimer and that the interaction of its monomers is important for its antiporter activity
Title: Oligomerization of the Saccharomyces cerevisiae Na+/H+ antiporter Nha1p: implications for its antiporter activity.
A detailed molecular mechanism for the Na+/H+ antiporter NHA1 is demonstrated, and the functional importance of domains proximal to the membrane region is discussed.
Title: A conserved domain in the tail region of the Saccharomyces cerevisiae Na+/H+ antiporter (Nha1p) plays important roles in localization and salinity-resistant cell-growth.
The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae.
Title: The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae.
Nha1p and its C-terminus in the immediate response to hyperosmotic shock as part of the rapid rescue mechanism.
Title: Functional study of the Nha1p C-terminus: involvement in cell response to changes in external osmolarity.
analysis of Nha1p transport activity using isolated secretory vesicles
Title: Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables potassium ion transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables sodium:proton antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium:proton antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables sodium:proton antiporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in intracellular monoatomic cation homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular potassium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular potassium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in potassium ion export across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proton export across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in regulation of membrane potential	IEA Inferred from Electronic Annotation more info
involved_in response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion export across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in sodium ion export across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info