U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    VPS33 tethering complex ATP-binding subunit VPS33 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851112, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    VPS33
    Official Full Name
    tethering complex ATP-binding subunit VPS33
    Primary source
    SGD:S000004388
    Locus tag
    YLR396C
    See related
    AllianceGenome:SGD:S000004388; FungiDB:YLR396C; VEuPathDB:YLR396C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CLS14; MET27; PEP14; SLP1; VAM5; VPL25; VPT33
    Summary
    Enables ATP binding activity. Contributes to phosphatidylinositol binding activity. Involved in several processes, including piecemeal microautophagy of the nucleus; vacuolar transport; and vacuole fusion. Located in cytosol and fungal-type vacuole membrane. Part of CORVET complex and HOPS complex. Human ortholog(s) of this gene implicated in arthrogryposis multiplex congenita; arthrogryposis, renal dysfunction, and cholestasis 1; cholestasis; kidney disease; and progressive familial intrahepatic cholestasis. Orthologous to several human genes including VPS33A (VPS33A core subunit of CORVET and HOPS complexes). [provided by Alliance of Genome Resources, Dec 2024]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See VPS33 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (910235..912310, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene SUMO ligase CST9 Neighboring gene cytochrome c oxidase subunit VIII Neighboring gene AAA family ATPase AFG2 Neighboring gene SKI complex RNA helicase subunit SKI2

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Intracellular distribution of amino acids in an slp1 vacuole-deficient mutant of the yeast Saccharomyces cerevisiae. Gent DP, et al. J Appl Microbiol, 1998 May. PMID 9674128
    2. The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function. Wada Y, et al. Mol Cell Biol, 1990 May. PMID 2183024, Free PMC Article
    3. Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Banta LM, et al. Mol Cell Biol, 1990 Sep. PMID 2201898, Free PMC Article
    4. Mutants of Saccharomyces cerevisiae with defective vacuolar function. Kitamoto K, et al. J Bacteriol, 1988 Jun. PMID 3131305, Free PMC Article
    5. The vacuolar compartment is required for sulfur amino acid homeostasis in Saccharomyces cerevisiae. Jacquemin-Faure I, et al. Mol Gen Genet, 1994 Sep 1. PMID 8078479

    See all (137) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17.
      Title: Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.
    2. Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
      Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.
    3. Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes
      Title: SM proteins Sly1 and Vps33 co-assemble with Sec17 and SNARE complexes to oppose SNARE disassembly by Sec18.
    4. Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
      Title: A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.
    5. Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified.
      Title: The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.
    6. we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association
      Title: Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.
    7. contribution of Vps33 to hemifusion and pore opening between vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing.
      Title: The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
    8. Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane
      Title: The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    contributes_to phosphatidylinositol binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Golgi to vacuole transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endocytosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular protein transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in piecemeal microautophagy of the nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein targeting to vacuole IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of SNARE complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vacuole fusion, non-autophagic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vacuole organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vesicle docking IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in vesicle fusion with vacuole IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in vesicle tethering IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vesicle-mediated transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in vesicle-mediated transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of CORVET complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of CORVET complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of HOPS complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in fungal-type vacuole IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in fungal-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole membrane IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    tethering complex ATP-binding subunit VPS33
    NP_013500.3
    • ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      910235..912310 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182284.3 → NP_013500.3  TPA: tethering complex ATP-binding subunit VPS33 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013500.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZ31, P20795
      UniProtKB/TrEMBL
      A7A1S1, B3RHR1, G2WJL1, N1P1U4
      Conserved Domains (1) summary
      COG5158
      Location:14 → 651
      SEC1; Proteins involved in synaptic transmission and general secretion, Sec1 family [Intracellular trafficking and secretion]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P20795.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools