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    RPN4 stress-regulated transcription factor RPN4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851542, updated on 9-Dec-2024

    Summary

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    Official Symbol
    RPN4
    Official Full Name
    stress-regulated transcription factor RPN4
    Primary source
    SGD:S000002178
    Locus tag
    YDL020C
    See related
    AllianceGenome:SGD:S000002178; FungiDB:YDL020C; VEuPathDB:YDL020C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    SON1; UFD5
    Summary
    Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in cellular response to arsenic-containing substance; regulation of DNA repair; and regulation of transcription by RNA polymerase II. Located in nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See RPN4 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (415113..416708, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Neighboring gene phosphoglycerate mutase family protein GPM2 Neighboring gene oxysterol-binding protein related protein OSH2 Neighboring gene Erp3p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. [Escherichia coli Dam methylase as a molecular tool for mapping binding sites of the yeast transcription factor Rpn4]. Spasskaia DS, et al. Mol Biol (Mosk), 2011 Jul-Aug. PMID 21954596
    2. Proteasomal degradation of RPN4 via two distinct mechanisms, ubiquitin-dependent and -independent. Ju D, et al. J Biol Chem, 2004 Jun 4. PMID 15090546
    3. [Rpn4p is a positive and negative transcriptional regulator of the ubiquitin-proteasome system]. Karpov DS, et al. Mol Biol (Mosk), 2008 May-Jun. PMID 18702311
    4. [Mapping of Rpn4p regions responsible for transcriptional activation of proteasome genes]. Karpov DS, et al. Mol Biol (Mosk), 2008 May-Jun. PMID 18702312
    5. Mapping of yeast Rpn4p transactivation domains. Karpov DS, et al. FEBS Lett, 2008 Oct 15. PMID 18804109

    See all (210) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Impairment of RPN4, a transcription factor, induces ER stress and lipid abnormality in Saccharomyces cerevisiae.
      Title: Impairment of RPN4, a transcription factor, induces ER stress and lipid abnormality in Saccharomyces cerevisiae.
    2. [Rpn4p without the DNA-Binding Domain Provides Saccharomyces cerevisiae Resistance to Oxidative Stress and Cycloheximide].
      Title: [Rpn4p without the DNA-Binding Domain Provides Saccharomyces cerevisiae Resistance to Oxidative Stress and Cycloheximide].
    3. The authors identify the proteasome biogenesis regulator Rpn4 and show that it cooperates with the unfolded protein response. Rpn4 abundance increases during endoplasmic reticulum stress, first by a post-transcriptional, then by a transcriptional mechanism.
      Title: The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance.
    4. Rpn4 induces TMC1 transcription in response to misfolded proteins. However, this response is counteracted by rapid proteasome-dependent degradation of Tmc1, which serves to normalize Tmc1 protein levels after induction
      Title: Tmc1 Is a Dynamically Regulated Effector of the Rpn4 Proteotoxic Stress Response.
    5. Rpn4-responsive elements in genes related to proteasome functions
      Title: Identification of minimum Rpn4-responsive elements in genes related to proteasome functions.
    6. under the methyl methanesulfonate stress Rpn4 promotes the regulation of several genes involved in DNA repair, antioxidant response and glucose metabolism.
      Title: [RPN4 the yeast transcription factor promotes the complex defence against methyi, methanesulfonate].
    7. the ubiquitin-independent degradation of Rpn4 requires the 19S regulatory particle.
      Title: The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits.
    8. evidence for the role of proteasomal degradation of Rpn4 in nonhomologous end-joining
      Title: Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks.
    9. Intracellular proteasome activity is correlated with the transactivation potency of Rpn4.
      Title: The transcription activation domain of Rpn4 is separate from its degrons.
    10. Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions.
      Title: Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions.
    Submit: New GeneRIF Correction See all GeneRIFs (21)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to arsenic-containing substance HMP PubMed 
    involved_in cellular response to arsenic-containing substance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to stress HMP PubMed 
    involved_in cellular response to stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II HMP PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II HMP PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    stress-regulated transcription factor RPN4
    NP_010264.3
    • Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      415113..416708 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180079.3 → NP_010264.3  TPA: stress-regulated transcription factor RPN4 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010264.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VRX0, E9P944, Q03465
      UniProtKB/TrEMBL
      C7GW59, C8Z4P9, N1P5X6
      Conserved Domains (1) summary
      sd00017
      Location:450 → 468
      ZF_C2H2; C2H2 Zn finger [structural motif]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03465.1

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