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    DAD4 Dad4p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851919, updated on 9-Dec-2024

    Summary

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    Official Symbol
    DAD4
    Official Full Name
    Dad4p
    Primary source
    SGD:S000007604
    Locus tag
    YDR320C-A
    See related
    AllianceGenome:SGD:S000007604; FungiDB:YDR320C-A; VEuPathDB:YDR320C-A
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HSK2
    Summary
    Contributes to microtubule plus-end binding activity. Involved in attachment of spindle microtubules to kinetochore and positive regulation of microtubule polymerization. Part of DASH complex. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See DAD4 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1108280..1108498, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Yft2p Neighboring gene auxilin-like protein SWA2 Neighboring gene asparaginase ASP1 Neighboring gene mitochondrial 54S ribosomal protein YmL35

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression. Ikeuchi A, et al. Biotechnol Prog, 2010 Jul-Aug. PMID 20730753
    2. The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Asbury CL, et al. Proc Natl Acad Sci U S A, 2006 Jun 27. PMID 16777964, Free PMC Article
    3. Protein arms in the kinetochore-microtubule interface of the yeast DASH complex. Miranda JJ, et al. Mol Biol Cell, 2007 Jul. PMID 17460120, Free PMC Article
    4. Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation. Gestaut DR, et al. Nat Cell Biol, 2008 Apr. PMID 18364702, Free PMC Article
    5. Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetries. Ramey VH, et al. J Struct Biol, 2009 Aug. PMID 19447181, Free PMC Article

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Results describe two novel subunits of DASH, Hsk2 and Hsk3 (helper of Ask1), and suggest that Hsk3 acts to incorporate Ask1 into the DASH complex.
      Title: Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    contributes_to microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to microtubule plus-end binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in attachment of spindle microtubules to kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in attachment of spindle microtubules to kinetochore IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome segregation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of microtubule polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DASH complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of DASH complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of DASH complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, centromeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in kinetochore IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in microtubule IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitotic spindle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in spindle IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Dad4p
    NP_710144.3
    • Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1108280..1108498 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184470.3 → NP_710144.3  TPA: Dad4p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_710144.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSV2, P69851
      UniProtKB/TrEMBL
      A6ZYS2, B3LFX7, C7GWL8, C8Z5N2, G2WB59, N1P590
      Conserved Domains (1) summary
      pfam08650
      Location:1 → 71
      DASH_Dad4; DASH complex subunit Dad4
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P69851.1

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