CDC24 Rho family guanine nucleotide exchange factor CDC24 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CDC24
Official Full Name: Rho family guanine nucleotide exchange factor CDC24
Primary source: SGD:S000000039
Locus tag: YAL041W
See related: AllianceGenome:SGD:S000000039; FungiDB:YAL041W; VEuPathDB:YAL041W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CLS4
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in several processes, including cellular bud site selection; chemotropism; and regulation of exit from mitosis. Acts upstream of or within pheromone-dependent signal transduction involved in conjugation with cellular fusion. Located in several cellular components, including cellular bud tip; incipient cellular bud site; and mating projection tip. Part of Cdc24p-Far1p-Gbetagamma complex. Human ortholog(s) of this gene implicated in cerebral atherosclerosis; cerebral infarction; and coronary artery disease. Orthologous to human KALRN (kalirin RhoGEF kinase) and PLEKHG4B (pleckstrin homology and RhoGEF domain containing G4B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CDC24 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (62840..65404)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cdc24 interacts with septins to create a positive feedback loop during bud site assembly in yeast.
Title: Cdc24 interacts with septins to create a positive feedback loop during bud site assembly in yeast.
Using optogenetics, the authors probe yeast polarization and find that local recruitment of Cdc24 or Bem1 is sufficient to induce polarization by triggering self-sustaining Cdc42 activity.
Title: Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization.
Biochemical analysis confirmed that this mutation disturbs the interaction with Bem1 but not the fold of the protein. The larger dataset obtained by NGS achieved a more complete representation of the bipartite interaction interface of Cdc24.
Title: A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations.
We propose that the NH2-terminal region unmasks the DH and PB1 domains, leading to the activation of Cdc42 and interaction with Bem1, respectively, to initiate cell polarization
Title: Essential role of the NH2-terminal region of Cdc24 guanine nucleotide exchange factor in its initial polarized localization in Saccharomyces cerevisiae.
These data support roles for Boi2p, Ent2p, Hua1p, Tos2p, and for Cdc24p phosphorylation in targeting Cdc24p to growth sites.
Title: Multiple proteins and phosphorylations regulate Saccharomyces cerevisiae Cdc24p localization.
study presents the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy
Title: NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae.
Cdc24p oligomerization regulates Cdc42p activation via its localization
Title: Oligomerization regulates the localization of Cdc24, the Cdc42 activator in Saccharomyces cerevisiae.
Results indicate that the exchange factor Cdc24p is necessary for maintaining or restricting specific proteins required for cell fusion to the cell contact region during mating.
Title: The exchange factor Cdc24 is required for cell fusion during yeast mating.
two-step molecular mechanism for the site-specific activation of Cdc24p, which involves Rsr1p/Bud1p and the adaptor protein Bem1p
Title: The nucleotide exchange factor Cdc24p may be regulated by auto-inhibition.
Gbetagamma not only targets Far1p to the correct site but may also trigger a conformational change in Far1p that is required for its ability to activate Cdc24p in vivo
Title: Site-specific regulation of the GEF Cdc24p by the scaffold protein Far1p during yeast mating.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular bud site selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chemotropism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pheromone-dependent signal transduction involved in conjugation with cellular fusion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cell cortex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone triggering conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septin ring organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in septin ring organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cdc24p-Far1p-Gbetagamma complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cdc24p-Far1p-Gbetagamma complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in division septum	IBA Inferred from Biological aspect of Ancestor more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
is_active_in mating projection tip	IBA Inferred from Biological aspect of Ancestor more info
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rho family guanine nucleotide exchange factor CDC24

NP_009359.1

Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001133.9 Reference assembly

Range

62840..65404

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178186.1 → NP_009359.1 TPA: Rho family guanine nucleotide exchange factor CDC24 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009359.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPH5, P11433

UniProtKB/TrEMBL

B3LUX7, C7GPJ6, C8Z3E8, G2W8I9, N1P7H4

Conserved Domains (4) summary

smart00325
Location:282 → 453

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

smart00666
Location:779 → 853

PB1; PB1 domain

cd13246
Location:455 → 667

PH_Scd1; Shape and Conjugation Deficiency 1 Pleckstrin homology (PH) domain

pfam06395
Location:156 → 242

CDC24; CDC24 Calponin