RAD54 DNA-dependent ATPase RAD54 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD54
Official Full Name: DNA-dependent ATPase RAD54
Primary source: SGD:S000003131
Locus tag: YGL163C
See related: AllianceGenome:SGD:S000003131; FungiDB:YGL163C; VEuPathDB:YGL163C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: XRS1
Summary: Enables DNA translocase activity. Involved in chromatin remodeling; chromosome organization; and heteroduplex formation. Located in nucleus. Human ortholog(s) of this gene implicated in breast cancer; disease of cellular proliferation; non-Hodgkin lymphoma; and pancreatic cancer. Orthologous to human RAD54L (RAD54 like). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RAD54 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (193707..196403, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Title: Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.
Title: Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.
Data suggest that recombinase RAD51 (Rad51)/DNA-dependent ATPase RAD54 (Rad54) form a functional unit operating in homology search, synaptic complex and D-loop formation.
Title: In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation.
ad51-mediated recombination occurring in the absence of Hed1 phosphorylation results in a significant increase in non-exchange chromosomes despite wild-type levels of crossovers, confirming previous results indicating a defect in crossover assurance. We propose that Rad51 function in meiosis is regulated in part by the coordinated phosphorylation of Rad54 and Hed1 by Mek1
Title: Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1.
Our study identified a novel sequence motif critical for Rad54 function and showed that even perfect matches to the PIP box consensus may not necessarily identify PCNA interaction sites.
Title: A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity.
Rad54 acts as an hDNA pump that drives D loop formation while simultaneously removing Rad51 from hDNA, consolidating both ATP-dependent activities of Rad54 into a single mechanistic step.
Title: Rad54 functions as a heteroduplex DNA pump modulated by its DNA substrates and Rad51 during D loop formation.
SN specifically targets the RAD54 ATPase, but not DNA binding, through direct interaction with RAD54 and generation of reactive oxygen species.
Title: Analysis of the activities of RAD54, a SWI2/SNF2 protein, using a specific small-molecule inhibitor.
Rad51-Rad54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange.
Title: Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.
Study identified a host factor, RAD54, in a screen for replication initiator protein (Rep)-interacting partners and observed its role in DNA replication of the geminivirus mungbean yellow mosaic India virus (MYMIV).
Title: A novel role for RAD54: this host protein modulates geminiviral DNA replication.
Rad54 and Rdh54 have different in vivo interactions with Rad51.
Title: Analyses of the yeast Rad51 recombinase A265V mutant reveal different in vivo roles of Swi2-like factors.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA translocase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA translocase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA translocase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on acid anhydrides	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA geometric change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in double-strand break repair via synthesis-dependent strand annealing	IBA Inferred from Biological aspect of Ancestor more info
involved_in heteroduplex formation	IDA Inferred from Direct Assay more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance via recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: DNA-dependent ATPase RAD54

NP_011352.1

EC 3.6.4.12

DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; drives ATP-hydrolysis-dependent sequence alignment during homologous recombination; involved in repair of dsDNA breaks during both mitosis and meiosis; promotes strand invasion, catalyzes nucleosome remodeling, and removes Rad51p from dsDNA; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

193707..196403 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181028.1 → NP_011352.1 TPA: DNA-dependent ATPase RAD54 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011352.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTY8, P32863

UniProtKB/TrEMBL

C7GPP3, C8Z888, N1P2X2

Conserved Domains (3) summary

COG0553
Location:148 → 827

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

cd18067
Location:271 → 554

DEXHc_RAD54A; DEXH-box helicase domain of RAD54A

pfam08658
Location:29 → 246

Rad54_N; Rad54 N terminal