PET9 ADP/ATP carrier protein PET9 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PET9
Official Full Name: ADP/ATP carrier protein PET9
Primary source: SGD:S000000126
Locus tag: YBL030C
See related: AllianceGenome:SGD:S000000126; FungiDB:YBL030C; VEuPathDB:YBL030C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AAC2; ANC2
Summary: Enables ATP:ADP antiporter activity and ATPase-coupled transmembrane transporter activity. Involved in several processes, including cellular respiration; heme transport; and purine ribonucleotide transport. Located in mitochondrial inner membrane. Is active in peroxisomal membrane. Used to study hypertrophic cardiomyopathy; lactic acidosis; mitochondrial DNA depletion syndrome (multiple); and myopathy (multiple). Human ortholog(s) of this gene implicated in intrinsic cardiomyopathy (multiple); mitochondrial DNA depletion syndrome (multiple); and mitochondrial myopathy (multiple). Orthologous to several human genes including SLC25A4 (solute carrier family 25 member 4) and SLC25A5 (solute carrier family 25 member 5). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PET9 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (163041..163997, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast aac2 R96H and aac2 R252G mutations are equivalent to R80H and R235G human ANT1 pathological mutations. mtDNA instability induced by aac2R96H and aac2R252G is rescued by N-acetylcysteine.
Title: Dominance of yeast aac2(R96H) and aac2(R252G) mutations, equivalent to pathological mutations in ant1, is due to gain of function.
Mutations in yeast Aac2, equivalent to those in human Ant1 in progressive external ophthalmoplegia, cause protein misfolding. Misfolded Aac2 affects assembly & stability of multiple protein complexes in membrane, which ultimately inhibits cell growth
Title: Misfolding of mutant adenine nucleotide translocase in yeast supports a novel mechanism of Ant1-induced muscle diseases.
The protective role of cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p).
Title: The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p).
Yeast ADP/ATP carrier isoform 2: conformational dynamics and role of the RRRMMM signature sequence methionines
Title: Yeast ADP/ATP carrier isoform 2: conformational dynamics and role of the RRRMMM signature sequence methionines.
Pathogenic mutations likely enhance the intrinsic proton-conducting activity of adenine nucleotide translocase Aac2, which excessively uncouples the mitochondrial inner membrane thereby affecting energy transduction and mitochondrial biogenesis.
Title: Dominant membrane uncoupling by mutant adenine nucleotide translocase in mitochondrial diseases.
Aac2 exists in physical association with the cytochrome bc1-COX supercomplex and the TIM23 machinery
Title: The yeast Aac2 protein exists in physical association with the cytochrome bc1-COX supercomplex and the TIM23 machinery.
Tyr203 and Phe208, aromatic residues of Anc2 transmembrane domain 4 protein carrier, may play a role in nucleotide attraction and its progress through the carrier leading to a productive adenosine diphosphate/adenosine triphosphate exchange.
Title: Two residues of a conserved aromatic ladder of the mitochondrial ADP/ATP carrier are crucial to nucleotide transport.
Pleiotropic effects of the yeast Sal1 and Aac2 carriers on mitochondrial function via an activity distinct from adenine nucleotide transport.
Title: Pleiotropic effects of the yeast Sal1 and Aac2 carriers on mitochondrial function via an activity distinct from adenine nucleotide transport.
the Anc2p C-terminal region is involved in nucleotide recognition, binding, and transport
Title: Structure-function relationships of the C-terminal end of the Saccharomyces cerevisiae ADP/ATP carrier isoform 2.
the residual transport activity in the presence of MTSES depended linearly on the amount of cysteine-less carrier. Thus, the entire transport cycle for ADP/ATP exchange is carried out by the monomer
Title: The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP:ADP antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP:ADP antiporter activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables cardiolipin binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in ADP transport	IDA Inferred from Direct Assay more info	PubMed
involved_in ATP transport	IDA Inferred from Direct Assay more info	PubMed
involved_in aerobic respiration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anaerobic respiration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heme transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial ADP transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial ATP transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in peroxisomal membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ADP/ATP carrier protein PET9

NP_009523.1

Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; also functions in peroxisomes; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

163041..163997 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178270.1 → NP_009523.1 TPA: ADP/ATP carrier protein PET9 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009523.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPW9, P18239

UniProtKB/TrEMBL

A6ZKR7, B3LNG8, C7GND2, G2W8X8, N1PAX7

Conserved Domains (1) summary

PTZ00169
Location:22 → 312

PTZ00169; ADP/ATP transporter on adenylate translocase; Provisional