Aloxe3 arachidonate lipoxygenase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Aloxe3provided by MGI
Official Full Name: arachidonate lipoxygenase 3provided by MGI
Primary source: MGI:MGI:1345140
See related: Ensembl:ENSMUSG00000020892 AllianceGenome:MGI:1345140
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: eLOX-3; e-LOX-3
Summary: Enables hepoxilin A3 synthase activity. Involved in several processes, including establishment of skin barrier; long-chain fatty acid metabolic process; and peroxisome proliferator activated receptor signaling pathway. Predicted to be located in cytoplasm. Is expressed in epidermis and skin. Human ortholog(s) of this gene implicated in autosomal recessive congenital ichthyosis 1 and autosomal recessive congenital ichthyosis 3. Orthologous to human ALOXE3 (arachidonate epidermal lipoxygenase 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in stomach adult (RPKM 6.6), CNS E18 (RPKM 2.7) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B3; 11 42.38 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (69016243..69039941)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (69125417..69149115)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study demonstrated that the epidermal-type lipoxygenase, eLOX3 (encoded by its gene, Aloxe3) is a fasting-responsive hepatocyte effector that is sufficient to attenuate insulin resistance, weight gain, and hepatic fat deposition in dietary and genetically obese models, in part by activating hepatic PPARgamma.
Title: Hepatocyte ALOXE3 is induced during adaptive fasting and enhances insulin sensitivity by activating hepatic PPARγ.
Loss-of-function mutations in the LOX genes ALOX12B and ALOXE3 have been found to represent the second most common cause of autosomal recessive congenital ichthyosis. [review]
Title: The role of lipoxygenases in epidermis.
Aloxe3 deficiency in mice resulted in early postnatal death. Skin lipid analysis demonstrated that the severity of barrier failure is related to the loss of covalently bound ceramides in both ALOX12B- and Aloxe3-null mice.
Title: Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation.
expression of eLOX3 or addition of eLOX3 products stimulated adipogenesis under conditions that normally require an exogenous PPAR gamma ligand for differentiation
Title: Epidermis-type lipoxygenase 3 regulates adipocyte differentiation and peroxisome proliferator-activated receptor gamma activity.
findings suggest that endogenous PPARgamma ligand(s) promoting adipocyte differentiation are generated via a baicalein-sensitive pathway involving the novel eLOX-3
Title: Adipocyte differentiation of 3T3-L1 preadipocytes is dependent on lipoxygenase activity during the initial stages of the differentiation process.
The 12R-LOX-eLOX-3 pathway plays a key role in the process of epidermal barrier acquisition by affecting lipid metabolism, as well as protein processing.
Title: 12R-lipoxygenase deficiency disrupts epidermal barrier function.
The substrate preference of mouse eLOX3 and the unique occurrence of an 8S-LOX enzyme in mouse skin point to a potential LOX pathway for the production of epoxyalcohol in murine epidermal differentiation.
Title: Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hepoxilin A3 synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hepoxilin A3 synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables hepoxilin A3 synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hepoxilin A3 synthase activity	ISO Inferred from Sequence Orthology more info
enables hydroperoxy icosatetraenoate dehydratase activity	IEA Inferred from Electronic Annotation more info
enables hydroperoxy icosatetraenoate isomerase activity	IEA Inferred from Electronic Annotation more info
enables intramolecular hydroxytransferase activity	ISO Inferred from Sequence Orthology more info
enables intramolecular hydroxytransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in arachidonate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arachidonate metabolic process	ISO Inferred from Sequence Orthology more info
NOT involved_in bicellular tight junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of skin barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of skin barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hepoxilin biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in hepoxilin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in hepoxilin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hepoxilin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in linoleic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid oxidation	IEA Inferred from Electronic Annotation more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in lipoxygenase pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in peroxisome proliferator activated receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of pain	ISO Inferred from Sequence Orthology more info
involved_in sensory perception of pain	ISS Inferred from Sequence or Structural Similarity more info
involved_in sphingolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in sphingolipid metabolic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: hydroperoxide isomerase ALOXE3

Names: epidermal LOX-3; epidermis-type lipoxygenase 3; hydroperoxy dehydratase ALOXE3; hydroperoxy icosatetraenoate dehydratase; hydroperoxy icosatetraenoate isomerase

NP_035916.2

EC 4.2.1.152

EC 5.4.4.7

XP_030101841.1

EC 4.2.1.152

EC 5.4.4.7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011786.2 → NP_035916.2 hydroperoxide isomerase ALOXE3

See identical proteins and their annotated locations for NP_035916.2

Status: VALIDATED

Source sequence(s)

AK141003, AL645527, BC116259

Consensus CDS

CCDS24884.1

UniProtKB/Swiss-Prot

B1ASX3, Q9WV07

UniProtKB/TrEMBL

Q14B96

Related

ENSMUSP00000021268.3, ENSMUST00000021268.9

Conserved Domains (2) summary

cd01753
Location:2 → 116

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:268 → 702

Lipoxygenase; Lipoxygenase