YUH1 ubiquitin-specific protease YUH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YUH1
Official Full Name: ubiquitin-specific protease YUH1
Primary source: SGD:S000003860
Locus tag: YJR099W
See related: AllianceGenome:SGD:S000003860; FungiDB:YJR099W; VEuPathDB:YJR099W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cysteine-type deubiquitinase activity. Predicted to be involved in protein catabolic process. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in Alzheimer's disease; Parkinson's disease; hereditary spastic paraplegia 79A; and hereditary spastic paraplegia 79B. Orthologous to several human genes including UCHL1 (ubiquitin C-terminal hydrolase L1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See YUH1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (615576..616286)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Following mutagenesis and yeast display screening, this study identified a variant of the yeast ubiquitin C-terminal hydrolase Yuh1 that has a 28-fold improvement in the transamidation to hydrolysis ratio relative to the wild type enzyme. The switch in activity enables robust autoubiquitination of a lysine in the crossover loop to form an isopeptide bond.
Title: Reprogramming a Deubiquitinase into a Transamidase.
Data show that upon complexation of ubiquitin with YUH1, 13C NMR paramagnetic shifts associated with the protein binding interface were reduced by 0.05 ppm or more.
Title: An NMR method for the determination of protein binding interfaces using TEMPOL-induced chemical shift perturbations.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ha3112 (e-PCR)
- UniSTS:515652

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin recycling	IC Inferred by Curator more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IC Inferred by Curator more info	PubMed

General protein information

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Preferred Names: ubiquitin-specific protease YUH1

NP_012633.1

EC 3.4.19.12

Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

615576..616286

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181757.1 → NP_012633.1 TPA: ubiquitin-specific protease YUH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012633.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWR8, P35127

UniProtKB/TrEMBL

A6ZQ55, B3LQI4, B5VLP6, C7GMA8, C8ZBP6

Conserved Domains (1) summary

cl08306
Location:8 → 231

Peptidase_C12; Cysteine peptidase C12 contains ubiquitin carboxyl-terminal hydrolase (UCH) families L1, L3, L5 and BAP1