Gpam glycerol-3-phosphate acyltransferase, mitochondrial [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gpamprovided by RGD
Official Full Name: glycerol-3-phosphate acyltransferase, mitochondrialprovided by RGD
Primary source: RGD:61847
See related: EnsemblRapid:ENSRNOG00000015124 AllianceGenome:RGD:61847
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables glycerol-3-phosphate O-acyltransferase activity. Involved in several processes, including cellular response to insulin stimulus; glycerolipid biosynthetic process; and positive regulation of triglyceride biosynthetic process. Located in mitochondrial outer membrane. Biomarker of metabolic dysfunction-associated steatotic liver disease; nephrotic syndrome; and obesity. Orthologous to human GPAM (glycerol-3-phosphate acyltransferase, mitochondrial). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Liver (RPKM 854.8), Heart (RPKM 350.2) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q55

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (264111599..264176112, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (254106323..254170755, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (275843823..275906880, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ChREBP and SREBP-1 bind to the mtGPAT distal promoter.
Title: Transcriptional regulation of mitochondrial glycerophosphate acyltransferase is mediated by distal promoter via ChREBP and SREBP-1.
Taken together, the results suggest that there are two promoters for rat mtGPAT gene and that the transcriptional regulation is mediated through the distal promoter.
Title: The presence of distal and proximal promoters for rat mitochondrial glycerol-3-phosphate acyltransferase.
These results demonstrate that two pools of GPAT1 are present in rat liver mitochondria: an active one, located in outer mitochondrial membrane and a less active one, located in membranes associated with both mitochondria and endoplasmic reticulum.
Title: Mitochondrial glycerol-3-P acyltransferase 1 is most active in outer mitochondrial membrane but not in mitochondrial associated vesicles (MAV).
Gpam regulation in rats in response to exercise
Title: Coordinate regulation of malonyl-CoA decarboxylase, sn-glycerol-3-phosphate acyltransferase, and acetyl-CoA carboxylase by AMP-activated protein kinase in rat tissues in response to exercise.
Thus, in isolated hepatocytes, mtGPAT1 may synthesize a cytosolic pool of TAG that cannot be secreted.
Title: Mitochondrial glycerol-3-phosphate acyltransferase-1 directs the metabolic fate of exogenous fatty acids in hepatocytes.
Casein kinase II stimulates rat liver mitochondrial glycerophosphate acyltransferase activity
Title: Casein kinase II stimulates rat liver mitochondrial glycerophosphate acyltransferase activity.
Data show that anti-CD3 stimulation in rat splenic T-lymphocytes selectively increased mitochondrial glycerol-3-phosphate acyltransferase (GPAT) activity.
Title: Aging reduces glycerol-3-phosphate acyltransferase activity in activated rat splenic T-lymphocytes.
TXow mitochondrial isoforms have been identified, one being sensitive in phosphorylation in vitro.
Title: Phosphorylation regulates mitochondrial glycerol-3-phosphate-1 acyltransferase activity in T-lymphocytes.
Casein kinase 2 phosphorylates liver mitochondrial glycerol-3-phosphate acyltransferase.
Title: Phosphorylation of rat liver mitochondrial glycerol-3-phosphate acyltransferase by casein kinase 2.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables glycerol-3-phosphate O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycerol-3-phosphate O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycerol-3-phosphate O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables glycerol-3-phosphate O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycerol-3-phosphate O-acyltransferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in CDP-diacylglycerol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in activated T cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of activated T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in activation-induced cell death of T cells	IEA Inferred from Electronic Annotation more info
acts_upstream_of activation-induced cell death of T cells	ISO Inferred from Sequence Orthology more info
involved_in acyl-CoA metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within acyl-CoA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to virus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within defense response to virus	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fatty acid homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fatty acid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in fatty acid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycerophospholipid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glycerophospholipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of activation-induced cell death of T cells	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of activation-induced cell death of T cells	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylglycerol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylglycerol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylglycerol biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in phospholipid homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phospholipid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of activated T cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of activated T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of multicellular organism growth	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of multicellular organism growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of triglyceride biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cytokine production	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cytokine production	ISO Inferred from Sequence Orthology more info
involved_in response to activity	IDA Inferred from Direct Assay more info	PubMed
involved_in response to cadmium ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to fructose	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucose	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to glucose	ISO Inferred from Sequence Orthology more info
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient levels	IEP Inferred from Expression Pattern more info	PubMed
involved_in triglyceride biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in triglyceride biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in triglyceride biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within triglyceride metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: glycerol-3-phosphate acyltransferase 1, mitochondrial

Names: GPAT; GPAT-1

NP_058970.1

EC 2.3.1.15

XP_006231687.1

EC 2.3.1.15

XP_006231688.1

EC 2.3.1.15

XP_063143835.1

EC 2.3.1.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017274.1 → NP_058970.1 glycerol-3-phosphate acyltransferase 1, mitochondrial

See identical proteins and their annotated locations for NP_058970.1

Status: PROVISIONAL

Source sequence(s)

AF021348

UniProtKB/Swiss-Prot

O35349, P97564, P97565, P97566

UniProtKB/TrEMBL

A0A0G2K2U7

Related

ENSRNOP00000069320.1, ENSRNOT00000083215.3

Conserved Domains (2) summary

cd07993
Location:201 → 411

LPLAT_DHAPAT-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: GPAT-like

COG2937
Location:131 → 663

PlsB; Glycerol-3-phosphate O-acyltransferase [Lipid transport and metabolism]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

264111599..264176112 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063287765.1 → XP_063143835.1 glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X2

Related

ENSRNOP00000103828.1, ENSRNOT00000142012.1
XM_006231626.5 → XP_006231688.1 glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1

See identical proteins and their annotated locations for XP_006231688.1

UniProtKB/TrEMBL

A0A0G2JV22, A0A0G2K2U7, A6JHY2

Conserved Domains (2) summary

cd07993
Location:201 → 411

LPLAT_DHAPAT-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: GPAT-like

COG2937
Location:131 → 663

PlsB; Glycerol-3-phosphate O-acyltransferase [Lipid transport and metabolism]
XM_006231625.5 → XP_006231687.1 glycerol-3-phosphate acyltransferase 1, mitochondrial isoform X1

See identical proteins and their annotated locations for XP_006231687.1

UniProtKB/TrEMBL

A0A0G2JV22, A0A0G2K2U7, A6JHY2

Conserved Domains (2) summary

cd07993
Location:201 → 411

LPLAT_DHAPAT-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: GPAT-like

COG2937
Location:131 → 663

PlsB; Glycerol-3-phosphate O-acyltransferase [Lipid transport and metabolism]