rst roughest [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: rstprovided by FlyBase
Official Full Name: roughestprovided by FlyBase
Primary source: FLYBASE:FBgn0003285
Locus tag: Dmel_CG4125
See related: AllianceGenome:FB:FBgn0003285
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG4125; CT13684; Dmel\CG4125; IREE-C; Irre-C; irreC; IrreC; irreC-rst; IrreC-rst; IrreC-Rst; IrreC/Rst; IRREC/RST; IrrecC; irrecC-rst; Rst; RST; rst-irreC; rst-irrecC; Rst/IrreC; rst/irrecC; UB883
Summary: Enables PDZ domain binding activity. Involved in several processes, including chaeta morphogenesis; homophilic cell adhesion via plasma membrane adhesion molecules; and regulation of striated muscle tissue development. Located in adherens junction and plasma membrane. Is active in apical plasma membrane. Is expressed in several structures, including anlage in statu nascendi; anterior-posterior subdivision of organism; germ layer; imaginal disc; and pigment cell. Human ortholog(s) of this gene implicated in nephrotic syndrome type 23. Orthologous to human KIRREL1 (kirre like nephrin family adhesion molecule 1); KIRREL2 (kirre like nephrin family adhesion molecule 2); and KIRREL3 (kirre like nephrin family adhesion molecule 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 3C3-3C4; 1-2 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (2950399..2973834, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (2844432..2867867, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results uncover a significant but hitherto uncharacterized involvement of IRM components Hbs, Kirre, and specially Rst in ovarian development
Title: Expression of Hbs, Kirre, and Rst during Drosophila ovarian development.
The data reveal a novel requirement for Sac1 in promoting microtubule stability and suggest that Rst trafficking occurs in a microtubule- and exocyst-dependent manner.
Title: The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developing Drosophila eye.
the roles of Rst, Hbs, Kirre and SNS in the spacing of bristles of the anterior wing margin, are explored.
Title: The Cell Adhesion Molecules Roughest, Hibris, Kin of Irre and Sticks and Stones Are Required for Long Range Spacing of the Drosophila Wing Disc Sensory Sensilla.
Cindr recapitulates both proposed functions of its mammalian orthologs Cd2ap and Cin85: targeting the IgCAMs Rst and Hbs for endocytosis and stabilizing these heterophilic IgCAM complexes.
Title: Interactions between Drosophila IgCAM adhesion receptors and cindr, the Cd2ap/Cin85 ortholog.
Analysis of a cytoplasmic motif present in Kirre and IrreC/Rst revealed a functionally required, conserved 12 AA motif (KIN1 motif).
Title: Functional study of mammalian Neph proteins in Drosophila melanogaster.
Findings suggest a general mechanism by which rst and kirre expression could be fine tuned to optimize their redundant roles during development.
Title: rst transcriptional activity influences kirre mRNA concentration in the Drosophila pupal retina during the final steps of ommatidial patterning.
identified two cell-adhesion molecules: Hibris, which is expressed in post-mitotic lamina precursor cells; and Roughest, which is expressed on retinal axons.
Title: Recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the Drosophila retinotopic map.
A novel relationship is described between the Crumbs partner beta(Heavy)-spectrin (beta(H)), the zona adherens and Roughest.
Title: The cell adhesion molecule Roughest depends on beta(Heavy)-spectrin during eye morphogenesis in Drosophila.
describe the expression pattern and of Rst in wild type and rst(D) mutants, showing that downregulation in salivary glands at the beginning of pupal stage is an important factor in implementation of the autophagic program of this tissue in space and time
Title: Precise temporal regulation of roughest is required for correct salivary gland autophagic cell death in Drosophila.
rst gene has a role in the differentiation of secondary/tertiary pigment cell fate
Title: Requirement of the roughest gene for differentiation and time of death of interommatidial cells during pupal stages of Drosophila compound eye development.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0126 (e-PCR)
- UniSTS:44158

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables PDZ domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables cell adhesion molecule binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in chaeta morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in compound eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye retinal cell programmed cell death	TAS Traceable Author Statement more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	IGI Inferred from Genetic Interaction more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	TAS Traceable Author Statement more info	PubMed
involved_in myoblast fusion	TAS Traceable Author Statement more info	PubMed
involved_in regulation of striated muscle tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinal cell programmed cell death	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell-cell junction	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roughest

Names: C-Roughest; CG4125-PA; CG4125-PB; ChiasmC-roughest; Irregular-chiasm-C; irreC-roughest; irregular chiasm C-roughest; irregular chiasm C-roughest protein; irregular chiasm-C-roughest; irregular chiasmC-roughest; irregular optic chiasma C; irregular optic chiasma C/Roughest; rst-PA; rst-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

2950399..2973834 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001297906.1 → NP_001284835.1 roughest, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001284835.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B9EQQ5, Q08180, Q2VA88, Q2VA98, Q2VAC5, Q8MQQ1, Q9W4U1

UniProtKB/TrEMBL

X2JCF8

Conserved Domains (6) summary

cd05758
Location:428 → 524

Ig5_KIRREL3-like; Fifth immunoglobulin (Ig)-like domain of Kirrel (kin of irregular chiasm-like) 3 (also known as Neph2) and similar proteins

smart00410
Location:435 → 524

IG_like; Immunoglobulin like

pfam07679
Location:346 → 420

I-set; Immunoglobulin I-set domain

pfam08205
Location:135 → 225

C2-set_2; CD80-like C2-set immunoglobulin domain

pfam13927
Location:265 → 329

Ig_3; Immunoglobulin domain

cl11960
Location:42 → 114

Ig; Immunoglobulin domain
NM_080319.5 → NP_525058.2 roughest, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_525058.2

Status: REVIEWED

UniProtKB/Swiss-Prot

B9EQQ5, Q08180, Q2VA88, Q2VA98, Q2VAC5, Q8MQQ1, Q9W4U1

UniProtKB/TrEMBL

X2JCF8

Related

FBpp0070537

Conserved Domains (6) summary

cd05758
Location:428 → 524

Ig5_KIRREL3-like; Fifth immunoglobulin (Ig)-like domain of Kirrel (kin of irregular chiasm-like) 3 (also known as Neph2) and similar proteins

smart00410
Location:435 → 524

IG_like; Immunoglobulin like

pfam07679
Location:346 → 420

I-set; Immunoglobulin I-set domain

pfam08205
Location:135 → 225

C2-set_2; CD80-like C2-set immunoglobulin domain

pfam13927
Location:265 → 329

Ig_3; Immunoglobulin domain

cl11960
Location:42 → 114

Ig; Immunoglobulin domain