robo3 roundabout 3 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: robo3provided by FlyBase
Official Full Name: roundabout 3provided by FlyBase
Primary source: FLYBASE:FBgn0041097
Locus tag: Dmel_CG5423
See related: AllianceGenome:FB:FBgn0041097
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG14345; CG5423; CT17158; Dmel\CG5423; dRobo-3; robo; Robo; Robo 3; robo-3; Robo-3; Robo3; ROBO3
Summary: Involved in gonad development and nervous system development. Located in axon and plasma membrane. Is expressed in several structures, including antennal lobe glomerulus; embryonic/larval optic lobe; inner proliferation zone; medulla anlage; and photoreceptor neurons. Human ortholog(s) of this gene implicated in congenital nystagmus (multiple); hematologic cancer (multiple); panhypopituitarism; and vesicoureteral reflux. Orthologous to human ROBO1 (roundabout guidance receptor 1) and ROBO2 (roundabout guidance receptor 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 21F3-21F4; 2-2 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (1256417..1296596)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (1256417..1296596)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the role of Slit and Robo receptors in wiring Drosophila higher-order brain circuits and identified differences in the cellular and molecular mechanisms of Robo/Slit function, was investigated.
Title: Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex.
Data show that Robo and Robo2 promote midline repulsion, while Robo2 and Robo3 specify the position of longitudinal axon pathways.
Title: Slit/Robo-mediated axon guidance in Tribolium and Drosophila: divergent genetic programs build insect nervous systems.
The transient cell-specific expression of Robo3 plays a crucial role in establishing neural circuits in the Drosophila visual system by selectively regulating pathway choice for posterior-most R8 growth cones.
Title: Robo-3--mediated repulsive interactions guide R8 axons during Drosophila visual system development.
Slit and Robo-family proteins act to maintain the distinct cellular composition of the developing lamina and the lobula cortex
Title: Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
axon guidance molecules roundabout2 and roundabout3 (robo2/3) are necessary for serotonergic neuron differentiation
Title: robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation.
Robo3 expression is one of a suite of chordotonal neuron properties that depend on expression of the proneural gene atonal.
Title: Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system.
robo, robo2, and robo3 play a role in the guidance of sensory axons in the Drosophila embryo by both slit-dependent and slit-independent mechanisms.
Title: Roundabout gene family functions during sensory axon guidance in the drosophila embryo are mediated by both Slit-dependent and Slit-independent mechanisms.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Process	Evidence Code	Pubs
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central complex development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sarcomere organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic target inhibition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in synaptic target inhibition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roundabout 3

Names: CG5423-PA; CG5423-PC; robo; robo3-PA; robo3-PC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

1256417..1296596

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001382178.1 → NP_001368960.1 roundabout 3, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

M9PAZ7, Q9GPP6

Conserved Domains (4) summary

cd00063
Location:519 → 612

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam00041
Location:743 → 832

fn3; Fibronectin type III domain

cd00096
Location:23 → 27

Ig; Ig strand A [structural motif]

cl11960
Location:23 → 117

Ig; Immunoglobulin domain
NM_134748.4 → NP_608592.2 roundabout 3, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_608592.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9GPP6, Q9VPZ7

Related

FBpp0077587

Conserved Domains (5) summary

smart00408
Location:429 → 489

IGc2; Immunoglobulin C-2 Type

cd00063
Location:519 → 612

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:122 → 211

I-set; Immunoglobulin I-set domain

pfam00041
Location:743 → 832

fn3; Fibronectin type III domain

cl11960
Location:22 → 117

Ig; Immunoglobulin domain