cno canoe [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: cnoprovided by FlyBase
Official Full Name: canoeprovided by FlyBase
Primary source: FLYBASE:FBgn0259212
Locus tag: Dmel_CG42312
See related: AllianceGenome:FB:FBgn0259212
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: AF-6; anon-WO0172774.47; Canoe; CG2534; CG31537; CG42312; Cno; cno?; dlhA; Dmel\CG42312; EC3-10; lip; mis
Old locus tag: Dmel_CG2534; Dmel_CG31537
Summary: Enables protein domain specific binding activity and small GTPase binding activity. Involved in several processes, including morphogenesis of an epithelium; regulation of signal transduction; and second mitotic wave involved in compound eye morphogenesis. Located in several cellular components, including adherens junction; cytoplasmic side of plasma membrane; and rhabdomere. Is expressed in several structures, including epithelium; germ layer; gut section; larval CNS neuron; and larval optic anlage. Orthologous to human AFDN (afadin, adherens junction formation factor). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 82F3-82F6; 3-47.5 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (5170004..5217456, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (998202..1043178, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Exploring the evolution and function of Canoe's intrinsically disordered region in linking cell-cell junctions to the cytoskeleton during embryonic morphogenesis.
Title: Exploring the evolution and function of Canoe's intrinsically disordered region in linking cell-cell junctions to the cytoskeleton during embryonic morphogenesis.
Abl and Canoe/Afadin mediate mechanotransduction at tricellular junctions.
Title: Abl and Canoe/Afadin mediate mechanotransduction at tricellular junctions.
Canoe and Polychaetoid stabilize Bazooka/Par3 at cell-cell junctions, helping maintain balanced apical contractility and tissue integrity.
Title: The Drosophila Afadin and ZO-1 homologues Canoe and Polychaetoid act in parallel to maintain epithelial integrity when challenged by adherens junction remodeling.
Rap1, Canoe and Mbt regulated epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the zonula adherens
Title: Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.
overexpression of cno simultaneously activates JNK and Ras-MEK-ERK signalling, resulting in mixed phenotypes of both overproliferation and cell death in the Drosophila wing disc.
Title: Polarity protein Canoe mediates overproliferation via modulation of JNK, Ras-MAPK and Hippo signalling.
Rap1-interacting domains of Canoe mediate adherens junction and tricellular junction recruitment but are dispensable for membrane localization. Data also support a role for Canoe multimerization. These multifactorial inputs shape Canoe localization, correct Bazooka and adherens junction positioning, and thus apical-basal polarity.
Title: Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment.
The complex ELMO-Sponge is subapically enriched and is required for subapical restriction of Canoe in early embryos. The localization dynamics of ELMO-Sponge suggests a patterning mechanism for positioning the subapical region adjacent to the apical region.
Title: ELMO and Sponge specify subapical restriction of Canoe and formation of the subapical domain in early Drosophila embryos.
the simultaneous loss of the asymmetric cell division regulators Canoe (afadin in mammals) and Scribble in neuroblast clones leads to tumor-like overgrowth through both a severe disruption of the asymmetric cell division process and canoe loss-mediated Ras-PI3K-Akt activation.
Title: Synergism between canoe and scribble mutations causes tumor-like overgrowth via Ras activation in neural stem cells and epithelia.
The Serrate-Notch-Canoe complex mediates interactions between glia and neuroepithelial cells during Drosophila optic lobe development.
Title: A Serrate-Notch-Canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development.
Data indicate that Rap1 and Cno are required for proper organization of Baz into planar-polarized junctional belts.
Title: Rap1 and Canoe/afadin are essential for establishment of apical-basal polarity in the Drosophila embryo.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables cell adhesion molecule binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in compound eye development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dorsal closure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermis morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glial cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ommatidial rotation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of JNK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment of planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of protein localization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in second mitotic wave involved in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in adherens junction	IBA Inferred from Biological aspect of Ancestor more info
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	HDA more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in rhabdomere	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: canoe

Names: CG42312-PC; CG42312-PD; CG42312-PE; CG42312-PF; CG42312-PG; CG42312-PH; CG42312-PI; CG42312-PJ; afadin; cno-PC; cno-PD; cno-PE; cno-PF; cno-PG; cno-PH; cno-PI; cno-PJ; dorsal holes A; lips; misty

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

5170004..5217456 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001316565.1 → NP_001303494.1 canoe, isoform J [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q95TT5, Q9VN82

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:1009 → 1097

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:983 → 1112

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:631 → 941

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_169025.3 → NP_730891.1 canoe, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730891.1

Status: REVIEWED

UniProtKB/TrEMBL

Q24279, Q8IPP4

Related

FBpp0288987

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:840 → 928

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:814 → 943

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:495 → 772

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_001275343.1 → NP_001262272.1 canoe, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262272.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGI6, Q95TT5

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:909 → 997

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:883 → 1012

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:531 → 841

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_001275344.1 → NP_001262273.1 canoe, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262273.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KG43, Q95TT5

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:1009 → 1097

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:983 → 1112

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:631 → 941

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_079508.4 → NP_524232.2 canoe, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524232.2

Status: REVIEWED

UniProtKB/TrEMBL

Q95TT5, Q9VN82

Related

FBpp0289752

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:1009 → 1097

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:983 → 1112

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:631 → 941

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_001275345.1 → NP_001262274.1 canoe, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262274.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KF82

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:1009 → 1097

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:983 → 1112

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:631 → 941

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain
NM_001275342.1 → NP_001262271.1 canoe, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262271.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFK9, Q95TT5

Conserved Domains (4) summary

smart00228
Location:679 → 767

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:653 → 782

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:301 → 611

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:68 → 131

FHA; FHA domain
NM_169026.3 → NP_730892.2 canoe, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730892.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8IPP3

Conserved Domains (6) summary

cd01781
Location:257 → 346

AF6_RA_repeat2; Ubiquitin domain of AT-6, second repeat

cd01782
Location:20 → 131

AF6_RA_repeat1; Ubiquitin domain of AT-6, first repeat

smart00228
Location:1009 → 1097

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG0265
Location:983 → 1112

DegQ; Periplasmic serine protease, S1-C subfamily, contain C-terminal PDZ domain [Posttranslational modification, protein turnover, chaperones]

cd15471
Location:631 → 941

Myo5p-like_CBD_afadin; cargo binding domain of myosin 5-like of afadin

pfam00498
Location:398 → 461

FHA; FHA domain

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4KF82		A0A0B4KF82
A0A0B4KFK9		A0A0B4KFK9
A0A0B4KG43		A0A0B4KG43
A0A0B4KGI6		A0A0B4KGI6
Q24279		Q24279
Q8IPP3		Q8IPP3
Q8IPP4		Q8IPP4
Q95TT5		Q95TT5
Q9VN82		Q9VN82