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    puf puffyeye [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 42935, updated on 9-Dec-2024

    Summary

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    Official Symbol
    pufprovided by FlyBase
    Official Full Name
    puffyeyeprovided by FlyBase
    Primary source
    FLYBASE:FBgn0039214
    Locus tag
    Dmel_CG5794
    See related
    AllianceGenome:FB:FBgn0039214
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    BcDNA:AT28817; CG5794; Dmel\CG5794; NEST:bs19d10; Puf; Q9VC56; Usp34; USP34
    Summary
    Predicted to enable cysteine-type deubiquitinase activity. Involved in several processes, including negative regulation of Toll signaling pathway; negative regulation of defense response; and positive regulation of double-strand break repair via homologous recombination. Located in nucleus. Is expressed in adult head and spermatozoon. Orthologous to human USP34 (ubiquitin specific peptidase 34). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See puf in Genome Data Viewer
    Location:
    96A13-96A13; 3-85 cM
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (24635270..24650043, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (20460992..20475765, complement)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene atlastin Neighboring gene wsck Neighboring gene Metallothionein F Neighboring gene absent, small, or homeotic discs 2 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene mir-1003 stem loop

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The ubiquitin thioesterase YOD1 ameliorates mutant Huntingtin induced pathology in Drosophila. Farkas A, et al. Sci Rep, 2023 Dec 11. PMID 38081944, Free PMC Article
    2. The Drosophila ubiquitin-specific protease Puffyeye regulates dMyc-mediated growth. Li L, et al. Development, 2013 Dec. PMID 24173801, Free PMC Article
    3. Model system identification of novel congenital heart disease gene candidates: focus on RPL13. Schroeder AM, et al. Hum Mol Genet, 2019 Dec 1. PMID 31625562, Free PMC Article
    4. Identifying USPs regulating immune signals in Drosophila: USP2 deubiquitinates Imd and promotes its degradation by interacting with the proteasome. Engel E, et al. Cell Commun Signal, 2014 Jul 16. PMID 25027767, Free PMC Article
    5. High throughput in vivo functional validation of candidate congenital heart disease genes in Drosophila. Zhu JY, et al. Elife, 2017 Jan 20. PMID 28084990, Free PMC Article

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster.
      Title: Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables cysteine-type deubiquitinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cysteine-type deubiquitinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine-type deubiquitinase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in negative regulation of Toll signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of antimicrobial peptide production IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of innate immune response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of peptidoglycan recognition protein signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of cell growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of cell growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of double-strand break repair via homologous recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in post-translational protein modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein deubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of protein stability IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of protein stability IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in wing disc development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    puffyeye
    Names
    CG5794-PD
    CG5794-PE
    CG5794-PF
    CG5794-PG
    CG5794-PH
    CG5794-PI
    puf-PD
    puf-PE
    puf-PF
    puf-PG
    puf-PH
    puf-PI
    NP_001262922.1
    NP_001262923.1
    NP_001287508.1
    NP_651275.1
    NP_733022.2
    NP_996287.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      24635270..24650043 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001275993.2 → NP_001262922.1  puffyeye, isoform G [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001262922.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4KHF3
      Conserved Domains (4) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:2014 → 2377
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam12030
      Location:2723 → 2877
      DUF3517; Domain of unknown function (DUF3517)
      cl19417
      Location:3671 → 3759
      FYDLN_acid; Protein of unknown function (FYDLN_acid)

    2. NM_170158.3 → NP_733022.2  puffyeye, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_733022.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8IMW2
      Conserved Domains (3) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:2014 → 2377
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam12030
      Location:2723 → 2877
      DUF3517; Domain of unknown function (DUF3517)

    3. NM_001300579.1 → NP_001287508.1  puffyeye, isoform I [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001287508.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4LIK5
      Conserved Domains (4) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:2014 → 2377
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam12030
      Location:2723 → 2877
      DUF3517; Domain of unknown function (DUF3517)
      cl19417
      Location:3671 → 3759
      FYDLN_acid; Protein of unknown function (FYDLN_acid)

    4. NM_143018.4 → NP_651275.1  puffyeye, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_651275.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q7KS15, Q9VC56
      Conserved Domains (2) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam10243
      Location:3651 → 3849
      MIP-T3; Microtubule-binding protein MIP-T3

    5. NM_001275994.2 → NP_001262923.1  puffyeye, isoform H [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001262923.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4KI06
      Conserved Domains (3) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:2014 → 2377
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam12030
      Location:2723 → 2877
      DUF3517; Domain of unknown function (DUF3517)

    6. NM_206564.2 → NP_996287.1  puffyeye, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_996287.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9VC56
      Conserved Domains (3) summary
      cd02659
      Location:2013 → 2382
      peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:2014 → 2377
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam12030
      Location:2723 → 2817
      DUF3517; Domain of unknown function (DUF3517)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4KHF3 A0A0B4KHF3
    A0A0B4KI06 A0A0B4KI06
    A0A0B4LIK5 A0A0B4LIK5
    Q8IH89 Q8IH89
    Q8IMW2 Q8IMW2
    Q8T8T3 Q8T8T3
    Q9VC56.2 Q9VC56

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