SPT16 chromatin-remodeling protein SPT16 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: SPT16
Official Full Name: chromatin-remodeling protein SPT16
Primary source: SGD:S000003175
Locus tag: YGL207W
See related: AllianceGenome:SGD:S000003175; FungiDB:YGL207W; VEuPathDB:YGL207W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC68; SSF1
Summary: Contributes to histone binding activity and nucleosome binding activity. Involved in several processes, including nucleosome assembly; positive regulation of RNA polymerase II transcription preinitiation complex assembly; and regulation of sister chromatid cohesion. Located in chromatin. Part of FACT complex and replication fork protection complex. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with dysmorphic facies and thin corpus callosum. Orthologous to human SUPT16H (SPT16 homolog, facilitates chromatin remodeling subunit). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See SPT16 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (98969..102076)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling.
Title: The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling.
Evolutionary conservation of protein dynamics: insights from all-atom molecular dynamics simulations of 'peptidase' domain of Spt16.
Title: Evolutionary conservation of protein dynamics: insights from all-atom molecular dynamics simulations of 'peptidase' domain of Spt16.
Dominant effects of the histone mutant H3-L61R on Spt16-gene interactions in budding yeast.
Title: Dominant effects of the histone mutant H3-L61R on Spt16-gene interactions in budding yeast.
Results provided evidence that the dissociation of Spt16, and by extension the FACT complex, from the 3' ends of some transcribed genes in yeast is in part dependent on Pol II termination.
Title: Evidence that dissociation of Spt16 from transcribed genes is partially dependent on RNA Polymerase II termination.
This study confirmed the interaction between cohesin (containing SMC1) and FACT complex (containing Spt16) in metaphase arrests using cells that expressed SMC1-6HA and SPT16-9MYC to validate that cohesin physically interacts with this histone chaperone.
Title: FACT mediates cohesin function on chromatin.
The article identifies proteins that directly bind the acidic patch in Saccharomyces cerevisiae: histone H2A binds to Paf1 complex subunit Rtf1 and FACT subunit Spt16.
Title: The nucleosome acidic patch directly interacts with subunits of the Paf1 and FACT complexes and controls chromatin architecture in vivo.
Spt16p is required for heterochromatin mediated silencing
Title: Histone chaperone Spt16p is required for heterochromatin mediated silencing in budding yeast.
SPT16 interactions with nucleosome.
Title: Charged residues on the side of the nucleosome contribute to normal Spt16-gene interactions in budding yeast.
Results show that Spt16 depletion results in global changes in nucleosome fuzziness and position shifts and that the majority of these changes are suppressed by inhibiting RNA polymerase II supporting a model in which Spt16 has a role in maintaining local nucleosome stability.
Title: Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells.
These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation.
Title: The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to chromatin binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to histone binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to nucleosome binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication	IPI Inferred from Physical Interaction more info	PubMed
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleosome assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of sister chromatid cohesion	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of FACT complex	IBA Inferred from Biological aspect of Ancestor more info
part_of FACT complex	IEA Inferred from Electronic Annotation more info
part_of FACT complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of FACT complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: chromatin-remodeling protein SPT16

NP_011308.1

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

98969..102076

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181072.1 → NP_011308.1 TPA: chromatin-remodeling protein SPT16 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011308.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTU8, P32558

UniProtKB/TrEMBL

B3LHQ3, B5VIC6, C7GWY9

Conserved Domains (1) summary

COG5406
Location:1 → 1035

COG5406; Nucleosome binding factor SPN, SPT16 subunit [Transcription, Replication, recombination and repair, Chromatin structure and dynamics]