TRIM45 tripartite motif containing 45 [Homo sapiens (human)] - Gene

Summary

Official Symbol: TRIM45provided by HGNC
Official Full Name: tripartite motif containing 45provided by HGNC
Primary source: HGNC:HGNC:19018
See related: Ensembl:ENSG00000134253 MIM:609318; AllianceGenome:HGNC:19018
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RNF99
Summary: Enables ubiquitin protein ligase activity. Involved in negative regulation of inflammatory response; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein K48-linked ubiquitination. Located in cytosol; intercellular bridge; and nucleoplasm. Is active in cytoplasm. [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in endometrium (RPKM 2.4), thyroid (RPKM 2.2) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p13.1

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (117111060..117123927, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (117121483..117134348, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (117653682..117664371, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation.
Title: TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation.
TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
Title: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
Title: TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
These findings suggest that TRIM45 is a novel tumor suppressor that stabilizes and activates p53 in glioma.
Title: TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination.
The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
Title: The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
These findings suggest that TRIM45 acts as a repressor for the NF-kappaB signal and regulates cell growth.
Title: TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation.
TRIM45 may act as a new transcriptional repressor in mitogen-activated protein kinase signaling pathway
Title: TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10S275 (e-PCR)
- UniSTS:147992

D1S1423 (e-PCR)
- UniSTS:149619

D16S325 (e-PCR)
- UniSTS:147089

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in bone development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in intercellular bridge	IDA Inferred from Direct Assay more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM45

Names: ring finger protein 99

NP_001139107.1

EC 2.3.2.27

NP_079464.2

EC 2.3.2.27

XP_047286870.1

EC 2.3.2.27

XP_054194842.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_028008.1 RefSeqGene

Range

5041..15730

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001145635.2 → NP_001139107.1 E3 ubiquitin-protein ligase TRIM45 isoform 2

See identical proteins and their annotated locations for NP_001139107.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AK222901, AY669488, BC034943, BU165629, EC541819

Consensus CDS

CCDS44200.1

UniProtKB/TrEMBL

Q5T2K3

Related

ENSP00000358476.3, ENST00000369464.7

Conserved Domains (4) summary

smart00557
Location:391 → 479

IG_FLMN; Filamin-type immunoglobulin domains

cd00021
Location:189 → 226

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam00630
Location:391 → 476

Filamin; Filamin/ABP280 repeat

cl23765
Location:240 → 357

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_025188.4 → NP_079464.2 E3 ubiquitin-protein ligase TRIM45 isoform 1

See identical proteins and their annotated locations for NP_079464.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK222901, AY669488, BU165629, EC541819

Consensus CDS

CCDS893.1

UniProtKB/Swiss-Prot

Q53GN0, Q5T2K4, Q5T2K5, Q8IYV6, Q9H8W5

UniProtKB/TrEMBL

Q5T2K3

Related

ENSP00000256649.4, ENST00000256649.9

Conserved Domains (4) summary

smart00557
Location:399 → 497

IG_FLMN; Filamin-type immunoglobulin domains

cd00021
Location:189 → 226

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam00630
Location:397 → 494

Filamin; Filamin/ABP280 repeat

cl23765
Location:240 → 357

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits