RNF34 ring finger protein 34 [Homo sapiens (human)] - Gene

Summary

Official Symbol: RNF34provided by HGNC
Official Full Name: ring finger protein 34provided by HGNC
Primary source: HGNC:HGNC:17297
See related: Ensembl:ENSG00000170633 MIM:608299; AllianceGenome:HGNC:17297
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
Summary: The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
Expression: Ubiquitous expression in testis (RPKM 15.4), lymph node (RPKM 13.4) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 12q24.31

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (121400118..121424348)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (121366860..121415781)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (121837921..121862151)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study identified RNF34 as an important regulator of immunity and mitophagy by inducing the autophagic degradation of MAVS aggregates. RNF34 promoted the K63 to K27/K29 ubiquitination transition of MAVS and degraded activated MAVS oligomers. The loss of RNF34 led to defects in mitophagy. The RNF34-mediated mitochondrial quality control mechanism links the innate immune response, mitochondrial homeostasis, and infection.
Title: RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation.
Data indicate that E3 ligase RNF34 is associated with nucleotide-binding oligomerization domain-containing protein 1 (NOD1).
Title: The E3 ligase RNF34 is a novel negative regulator of the NOD1 pathway.
Data indicate AE induces caspase-8-mediated activation of mitochondrial death pathways by decreasing the stability of CARP mRNAs in a p53-independent manner.
Title: Destabilization of CARP mRNAs by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells.
The previously unknown small molecule inhibitor-dependent interaction of Cbeta1 with the cell cycle and apoptosis regulatory protein-1 was verified.
Title: Uncoupling of bait-protein expression from the prey protein environment adds versatility for cell and tissue interaction proteomics and reveals a complex of CARP-1 and the PKA Cbeta1 subunit.
CARPs together with MDM2 enhance p53 degradation, thereby inhibiting p53-mediated cell death.
Title: CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2.
hRFI may act as an oncogenic molecule affecting the apoptotic pathway
Title: Effects of expression of hRFI on adenoma formation and tumor progression in colorectal adenoma-carcinoma sequence.
a novel gene, hRFI, preferentially expressed in esophageal cancer (hRFI)
Title: Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.

Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G35016 (e-PCR)
- UniSTS:15912

RH64995 (e-PCR)
- UniSTS:84284

D12S1398 (e-PCR)
- UniSTS:32605

G62101 (e-PCR)
- UniSTS:139128

RNF36_1732 (e-PCR)
- UniSTS:277685

NIB1331 (e-PCR)
- UniSTS:58859

SGC32343 (e-PCR)
- UniSTS:69100

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables p53 binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphatidylinositol phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cold	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of signal transduction by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K48-linked ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of oxygen metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of signal transduction by p53 class mediator	TAS Traceable Author Statement more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in nuclear body	IDA Inferred from Direct Assay more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RNF34

Names: FYVE-RING finger protein MOMO; RING finger protein RIFF; RING-type E3 ubiquitin transferase RNF34; caspase regulator CARP1; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein; ring finger protein 34, E3 ubiquitin protein ligase

NP_001243787.1

EC 2.3.2.27

NP_001381137.1

EC 2.3.2.27

NP_079402.2

EC 2.3.2.27

NP_919247.1

EC 2.3.2.27

XP_024304959.1

EC 2.3.2.27

XP_047285520.1

EC 2.3.2.27

XP_054229254.1

EC 2.3.2.27

XP_054229255.1

EC 2.3.2.27

XP_054229256.1

EC 2.3.2.27

XP_054229257.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001256858.2 → NP_001243787.1 E3 ubiquitin-protein ligase RNF34 isoform 3

Status: REVIEWED

Source sequence(s)

AK025439, BI559962

Consensus CDS

CCDS73538.1

UniProtKB/TrEMBL

A0A087WTM5

Related

ENSP00000477987.1, ENST00000613529.4

Conserved Domains (1) summary

pfam13920
Location:129 → 171

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)
NM_001394208.1 → NP_001381137.1 E3 ubiquitin-protein ligase RNF34 isoform 1

Status: REVIEWED

Source sequence(s)

AC048337

Consensus CDS

CCDS9221.1

Conserved Domains (2) summary

cd15769
Location:60 → 106

FYVE_CARP1; FYVE-like domain found in caspase regulator CARP1 and similar proteins

cd16706
Location:325 → 363

RING-HC_CARP1; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein 1 (CARP1) and similar proteins
NM_025126.4 → NP_079402.2 E3 ubiquitin-protein ligase RNF34 isoform 2

See identical proteins and their annotated locations for NP_079402.2

Status: REVIEWED

Source sequence(s)

AF306709, AK025439

Consensus CDS

CCDS31915.1

UniProtKB/Swiss-Prot

B7Z933, Q8NG47, Q969K3, Q9H6W8

Related

ENSP00000355137.5, ENST00000361234.10

Conserved Domains (2) summary

cd15769
Location:59 → 105

FYVE_CARP1; FYVE-like domain found in caspase regulator CARP1 and similar proteins

cd16706
Location:324 → 362

RING-HC_CARP1; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein 1 (CARP1) and similar proteins
NM_194271.3 → NP_919247.1 E3 ubiquitin-protein ligase RNF34 isoform 1

See identical proteins and their annotated locations for NP_919247.1

Status: REVIEWED

Source sequence(s)

AF306709, AK025439, DA887995

Consensus CDS

CCDS9221.1

UniProtKB/Swiss-Prot

Q969K3

Related

ENSP00000376258.3, ENST00000392465.7

Conserved Domains (2) summary

cd15769
Location:60 → 106

FYVE_CARP1; FYVE-like domain found in caspase regulator CARP1 and similar proteins

cd16706
Location:325 → 363

RING-HC_CARP1; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein 1 (CARP1) and similar proteins

RNA

NR_172087.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC048337

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000012.12 Reference GRCh38.p14 Primary Assembly

Range

121400118..121424348

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047429564.1 → XP_047285520.1 E3 ubiquitin-protein ligase RNF34 isoform X1
XM_024449191.2 → XP_024304959.1 E3 ubiquitin-protein ligase RNF34 isoform X1

Conserved Domains (2) summary

cd15769
Location:52 → 98

FYVE_CARP1; FYVE-like domain found in caspase regulator CARP1 and similar proteins

cd16706
Location:317 → 355

RING-HC_CARP1; RING finger, HC subclass, found in caspases-8 and -10-associated RING finger protein 1 (CARP1) and similar proteins

Alternate T2T-CHM13v2.0

Genomic

NC_060936.1 Alternate T2T-CHM13v2.0

Range

121366860..121415781

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054373280.1 → XP_054229255.1 E3 ubiquitin-protein ligase RNF34 isoform X3

UniProtKB/Swiss-Prot

B7Z933, Q8NG47, Q969K3, Q9H6W8
XM_054373279.1 → XP_054229254.1 E3 ubiquitin-protein ligase RNF34 isoform X2
XM_054373282.1 → XP_054229257.1 E3 ubiquitin-protein ligase RNF34 isoform X1
XM_054373281.1 → XP_054229256.1 E3 ubiquitin-protein ligase RNF34 isoform X1