Serpinb9 serine (or cysteine) peptidase inhibitor, clade B, member 9 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Serpinb9provided by MGI
Official Full Name: serine (or cysteine) peptidase inhibitor, clade B, member 9provided by MGI
Primary source: MGI:MGI:106603
See related: Ensembl:ENSMUSG00000045827 AllianceGenome:MGI:106603
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PI9; CAP3; PI-9; Spi6; CAP-3; ovalbumin
Summary: Enables serine-type endopeptidase inhibitor activity. Acts upstream of or within several processes, including T cell mediated cytotoxicity; acute inflammatory response to antigenic stimulus; and negative regulation of T cell apoptotic process. Located in cytoplasm. Is expressed in several structures, including genitourinary system; heart; liver; lung; and spleen. Orthologous to human SERPINB9 (serpin family B member 9). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in lung adult (RPKM 6.6), placenta adult (RPKM 6.0) and 21 other tissues See more
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Genomic context

Location:: 13 A3.2- A3.3; 13 13.79 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (33181044..33201938)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (32997061..33017955)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer.
Title: In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer.
These findings add support for role for Sb9 cross-presentation, and indicate that high Sb9 expression is a novel marker of cross-presentation capable dendritic cells
Title: Serpinb9 is a marker of antigen cross-presenting dendritic cells.
Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9.
Title: A Novel Serpin Regulatory Mechanism: SerpinB9 IS REVERSIBLY INHIBITED BY VICINAL DISULFIDE BOND FORMATION IN THE REACTIVE CENTER LOOP.
our data demonstrate, for the first time, a novel role for Spi6 in Regulatory T cell homeostasis
Title: Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells.
Data indicate that Serpinb9 (Spi6)-deficient dendritic cells appear to process antigen faster.
Title: Serpinb9 (Spi6)-deficient mice are impaired in dendritic cell-mediated antigen cross-presentation.
the negative regulation of DC priming of CD8 T lymphocyte immunity by CTL killing is mitigated by the physiological inhibition of GrB by Spi6.
Title: Serine protease inhibitor 6 is required to protect dendritic cells from the kiss of death.
SPI6 protects mesenchymal stem cells from GrB-mediated killing and plays a pivotal role in their survival in vivo.
Title: Mesenchymal stem cells express serine protease inhibitor to evade the host immune response.
Data show that increased activation-induced apoptosis resulted in impaired survival and a decreased clonal burst size of Spi6 KO iNKT cells, which could be corrected by GrB deficiency.
Title: Serine protease inhibitor 6 protects iNKT cells from self-inflicted damage.
Increased SPI-6 expression is not sufficient to confer lipopolysaccharide-treated dendritic cells with resistance to direct killing in vivo.
Title: Dendritic cells treated with lipopolysaccharide up-regulate serine protease inhibitor 6 and remain sensitive to killing by cytotoxic T lymphocytes in vivo.
SPI-6 is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B.
Title: Intrahepatic lymphocyte expression of dipeptidyl peptidase I-processed granzyme B and perforin induces hepatocyte expression of serine proteinase inhibitor 6 (Serpinb9/SPI-6).

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB283241 (e-PCR)
- UniSTS:208915

U96700 (e-PCR)
- UniSTS:160281

RH142116 (e-PCR)
- UniSTS:124786

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase inhibitor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within T cell mediated cytotoxicity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within acute inflammatory response to antigenic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in immune response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of T cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in protection from natural killer cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serpin B9

Names: serine (or cysteine) proteinase inhibitor, clade B, member 9; serine protease inhibitor 6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.