Dgka diacylglycerol kinase, alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkaprovided by MGI
Official Full Name: diacylglycerol kinase, alphaprovided by MGI
Primary source: MGI:MGI:102952
See related: Ensembl:ENSMUSG00000025357 AllianceGenome:MGI:102952
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 80kDa; Dagk1
Summary: Enables ATP-dependent diacylglycerol kinase activity and phospholipid binding activity. Predicted to be involved in glycerolipid metabolic process; intracellular signal transduction; and lipid phosphorylation. Located in cytosol and plasma membrane. Is expressed in several structures, including alimentary system; cranial ganglion; integumental system; respiratory system; and sensory organ. Orthologous to human DGKA (diacylglycerol kinase alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in thymus adult (RPKM 101.8), spleen adult (RPKM 51.2) and 15 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D3; 10 77.14 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (128556000..128581002, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (128720131..128745084, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ablation of DGKalpha facilitates alpha-smooth muscle actin expression via the Smad and PKCdelta signaling pathways during the acute phase of CCl4 -induced hepatic injury.
Title: Ablation of DGKα facilitates α-smooth muscle actin expression via the Smad and PKCδ signaling pathways during the acute phase of CCl(4) -induced hepatic injury.
Lymphocytic Choriomeningitis Virus Clone 13 Infection Results in CD8 T Cell-Mediated Host Mortality in Diacylglycerol Kinase alpha-Deficient Mice.
Title: Lymphocytic Choriomeningitis Virus Clone 13 Infection Results in CD8 T Cell-Mediated Host Mortality in Diacylglycerol Kinase α-Deficient Mice.
Loss of Diacylglycerol Kinase alpha Enhances Macrophage Responsiveness.
Title: Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness.
DGK alpha and zeta Activities Control TH1 and TH17 Cell Differentiation.
Title: DGK α and ζ Activities Control T(H)1 and T(H)17 Cell Differentiation.
results suggest DGKalpha aids in modulating the early immune response of adipose tissue following an acute exposure to high-fat diet, possibly through modulation of acute T-cell action
Title: Diacylglycerol kinase α deficiency alters inflammation markers in adipose tissue in response to a high-fat diet.
this study shows that diacylglycerol kinase alpha is involved in the vitamin E-induced amelioration of diabetic nephropathy in mice
Title: Diacylglycerol Kinase alpha is Involved in the Vitamin E-Induced Amelioration of Diabetic Nephropathy in Mice.
Diacylglycerol kinases alpha and zeta are up-regulated in cancer in cancer, and contribute towards tumor immune evasion and T cells clonal anergy. (Review)
Title: Diacylglycerol kinases in cancer.
Our studies demonstrate that PKD1/2 is a key regulator of MVB maturation and exosome secretion, and constitutes a mediator of the DGK alpha effect on MVB secretory traffic.
Title: Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes.
Diacylglycerol kinase alpha establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse.
Title: Diacylglycerol kinase α establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse.
It plays a role in pathogenesis of various disorders in the central nervous system.
Title: The roles of diacylglycerol kinases in the central nervous system: review of genetic studies in mice.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (1) 1 citation
Transgenic (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW146112 (e-PCR)
- UniSTS:180131

Dgka (e-PCR), detects polymorphism
- UniSTS:506844

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables alkylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in lipid phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: diacylglycerol kinase alpha

Names: 80 kDa diacylglycerol kinase; DAG kinase alpha; DGK-alpha; diacylglycerol kinase alpha Flt2-Pr2; diglyceride kinase alpha

NP_001345674.1

EC 2.7.1.107

EC 2.7.1.93

NP_001345675.1

EC 2.7.1.107

EC 2.7.1.93

NP_001359295.1

EC 2.7.1.107

EC 2.7.1.93

NP_001360957.1

EC 2.7.1.107

EC 2.7.1.93

NP_058091.2

EC 2.7.1.107

EC 2.7.1.93

XP_011241653.2

EC 2.7.1.107

EC 2.7.1.93

XP_011241661.1

EC 2.7.1.107

EC 2.7.1.93

XP_011241665.2

EC 2.7.1.107

EC 2.7.1.93

XP_017169275.2

EC 2.7.1.107

EC 2.7.1.93

XP_017169279.2

EC 2.7.1.107

EC 2.7.1.93

XP_030100725.1

EC 2.7.1.107

EC 2.7.1.93

XP_036011466.1

EC 2.7.1.107

EC 2.7.1.93

XP_036011467.1

EC 2.7.1.107

EC 2.7.1.93

XP_036011468.1

EC 2.7.1.107

EC 2.7.1.93

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001358745.2 → NP_001345674.1 diacylglycerol kinase alpha isoform 1

Status: VALIDATED

Source sequence(s)

AC131081

Consensus CDS

CCDS24290.1

UniProtKB/Swiss-Prot

O88673, Q922X2

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:515 → 696

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:374 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:115 → 184

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:207 → 255

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 111

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001358746.2 → NP_001345675.1 diacylglycerol kinase alpha isoform 1

Status: VALIDATED

Source sequence(s)

AC131081

Consensus CDS

CCDS24290.1

UniProtKB/Swiss-Prot

O88673, Q922X2

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:515 → 696

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:374 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:115 → 184

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:207 → 255

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 111

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001372366.1 → NP_001359295.1 diacylglycerol kinase alpha isoform 2

Status: VALIDATED

Source sequence(s)

AC131081

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (6) summary

smart00045
Location:550 → 731

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:409 → 529

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:150 → 219

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 146

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:306 → 367

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:237 → 297

C1; protein kinase C conserved region 1 (C1 domain) superfamily
NM_001374028.2 → NP_001360957.1 diacylglycerol kinase alpha isoform 3

Status: VALIDATED

Source sequence(s)

AC131081

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:538 → 719

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:397 → 517

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:138 → 207

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:230 → 278

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 134

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001428430.1 → NP_001415359.1 diacylglycerol kinase alpha isoform 4

Status: VALIDATED

Source sequence(s)

AC131081
NM_001428431.1 → NP_001415360.1 diacylglycerol kinase alpha isoform 5

Status: VALIDATED

Source sequence(s)

AC131081
NM_001428432.1 → NP_001415361.1 diacylglycerol kinase alpha isoform 6

Status: VALIDATED

Source sequence(s)

AC131081
NM_016811.4 → NP_058091.2 diacylglycerol kinase alpha isoform 1

See identical proteins and their annotated locations for NP_058091.2

Status: VALIDATED

Source sequence(s)

AC131081

Consensus CDS

CCDS24290.1

UniProtKB/Swiss-Prot

O88673, Q922X2

UniProtKB/TrEMBL

R4I4W1

Related

ENSMUSP00000026414.8, ENSMUST00000026414.9

Conserved Domains (5) summary

smart00045
Location:515 → 696

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:374 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:115 → 184

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:207 → 255

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 111

DAG_kinase_N; Diacylglycerol kinase N-terminus

RNA

NR_190347.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC131081

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

128556000..128581002 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155574.1 → XP_036011467.1 diacylglycerol kinase alpha isoform X6

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:373 → 554

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:232 → 352

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:1 → 42

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd20890
Location:129 → 190

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:60 → 120

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_036155575.1 → XP_036011468.1 diacylglycerol kinase alpha isoform X6

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:373 → 554

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:232 → 352

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:1 → 42

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd20890
Location:129 → 190

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:60 → 120

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_030244865.2 → XP_030100725.1 diacylglycerol kinase alpha isoform X5

UniProtKB/Swiss-Prot

O88673, Q922X2

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:515 → 696

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:374 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:115 → 184

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:207 → 255

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 111

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_011243359.3 → XP_011241661.1 diacylglycerol kinase alpha isoform X4

See identical proteins and their annotated locations for XP_011241661.1

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (5) summary

smart00045
Location:538 → 719

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:397 → 517

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:138 → 207

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00130
Location:230 → 278

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam14513
Location:4 → 134

DAG_kinase_N; Diacylglycerol kinase N-terminus
XM_011243351.3 → XP_011241653.2 diacylglycerol kinase alpha isoform X3

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (6) summary

smart00045
Location:550 → 731

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:409 → 529

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:150 → 219

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 146

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:306 → 367

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:237 → 297

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_017313786.3 → XP_017169275.2 diacylglycerol kinase alpha isoform X2

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (6) summary

smart00045
Location:550 → 731

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:409 → 529

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:150 → 219

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 146

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:306 → 367

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:237 → 297

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_036155573.1 → XP_036011466.1 diacylglycerol kinase alpha isoform X1

UniProtKB/TrEMBL

R4I4W1

Conserved Domains (6) summary

smart00045
Location:573 → 754

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:432 → 552

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:173 → 242

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 169

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:329 → 390

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:260 → 320

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_017313790.2 → XP_017169279.2 diacylglycerol kinase alpha isoform X8

UniProtKB/TrEMBL

R4I3X7

Conserved Domains (4) summary

cd00051
Location:150 → 219

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 146

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:306 → 367

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:237 → 297

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_011243363.2 → XP_011241665.2 diacylglycerol kinase alpha isoform X7

UniProtKB/TrEMBL

R4I3X7

Conserved Domains (4) summary

cd00051
Location:173 → 242

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:39 → 169

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20890
Location:329 → 390

C1_DGKalpha_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase alpha (DAG kinase alpha) and similar proteins

cl00040
Location:260 → 320

C1; protein kinase C conserved region 1 (C1 domain) superfamily