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    rhlR transcriptional regulator RhlR [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 878968, updated on 24-Aug-2024

    Summary

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    Gene symbol
    rhlR
    Gene description
    transcriptional regulator RhlR
    Locus tag
    PA3477
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (3889925..3890650, complement)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene cyclohexadienyl dehydratase Neighboring gene acyl-homoserine-lactone synthase Neighboring gene rhamnosyltransferase subunit B Neighboring gene rhamnosyltransferase subunit A

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Identification of ligand binding activity and DNA recognition by RhlR protein from opportunistic pathogen Pseudomonas aeruginosa-a molecular dynamic simulation approach. Chowdhury N, et al. J Mol Recognit, 2018 Dec. PMID 29968257
    2. Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa. Lixa C, et al. Protein Expr Purif, 2016 May. PMID 26792557
    3. Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD. Malgaonkar A, et al. Sci Rep, 2019 Jan 23. PMID 30674910, Free PMC Article
    4. Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Ochsner UA, et al. J Bacteriol, 1994 Apr. PMID 8144472, Free PMC Article
    5. The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: multiple quorum sensing-regulated genes are modulated without affecting lasRI, rhlRI or the production of N-acyl-L-homoserine lactones. Déziel E, et al. Mol Microbiol, 2005 Feb. PMID 15686549

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The interaction of RhlR-5'UTR with PhrD in E. coli indicated that this regulation could function without the involvement of any Pseudomonas specific proteins.
      Title: Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD.
    2. Inactivation of RhlR protein (rhlR) of Pseudomonas aeruginosa does not block the quorum-sensing (QSR), nor impairs the virulence, and that even a LasR protein (lasR) rhlR double mutant presents residual virulence, even lacking the production of virulence factors. These results show that the inhibition of either lasR or rhlR is not a straightforward approach to blocking virulence, due to the great complexity of the QSR.
      Title: Inactivation of the quorum-sensing transcriptional regulators LasR or RhlR does not suppress the expression of virulence factors and the virulence of Pseudomonas aeruginosa PAO1.
    3. Study identifies the mechanism of the binding of the autoinducer BHL ligand to the dimeric RhlR protein, which would further help the dimeric RhlR protein to bind the cognate promoter DNA to initiate the transcription of virulence genes. Also, the key amino acid residues from the RhlR protein were identified to be involved in ligand binding, protein-protein interactions, and DNA binding.
      Title: Identification of ligand binding activity and DNA recognition by RhlR protein from opportunistic pathogen Pseudomonas aeruginosa-a molecular dynamic simulation approach.
    4. rhlI mutants are unexpectedly more virulent than rhlR mutants, both in fly and in nematode intestinal infection models, suggesting that RhlR has RhlI-independent functions
      Title: Quorum-sensing regulator RhlR but not its autoinducer RhlI enables Pseudomonas to evade opsonization.
    5. this work shows that PqsE and RhlR function as a QS-autoinducer synthase-receptor pair that drives group behaviors in P. aeruginosa.
      Title: The PqsE and RhlR proteins are an autoinducer synthase-receptor pair that control virulence and biofilm development in Pseudomonas aeruginosa.
    6. rhlR/lasR knockout increased the susceptibility of Pseudomonas aeruginosa towards polymyxin B-azithromycin combination via influencing quorum sensing.
      Title: Influence of rhlR and lasR on Polymyxin Pharmacodynamics in Pseudomonas aeruginosa and Implications for Quorum Sensing Inhibition with Azithromycin.
    7. This is the first time that a domain of the quorum sensing regulator RhlR was produced in sufficient amounts for structural studies, enabling the investigation of the molecular basis for RhlR specific interaction with DNA promoters.
      Title: Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa.
    8. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED.
      Title: Silver-zinc redox-coupled electroceutical wound dressing disrupts bacterial biofilm.
    9. Subinhibitory concentrations of macrolides specifically upregulated expression of quorum sensing genes (rhlR, lasI), whereas sulfonamides and municipal wastewater, instead upregulated expression of specific resistant genes (sul1) and efflux pumps (mexD).
      Title: Sub-inhibitory concentrations of antibiotics and wastewater influencing biofilm formation and gene expression of multi-resistant Pseudomonas aeruginosa wastewater isolates.
    10. The rmlBDAC operon has three promoters, and that the expression of the P2 promoter is dependent on the stationary phase sigma factor sigmaS and on the quorum sensing regulator RhlR.
      Title: The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor σS.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Process Evidence Code Pubs
    acts_upstream_of_or_within positive regulation of lipid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of proteolysis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of secondary metabolite biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    transcriptional regulator RhlR
    NP_252167.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      3889925..3890650 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_252167.1 transcriptional regulator RhlR [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_252167.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A411F6T9
      Conserved Domains (1) summary
      PRK10188
      Location:4 → 241
      PRK10188; transcriptional regulator SdiA
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P54292.1

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