Ube2w ubiquitin-conjugating enzyme E2W (putative) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2wprovided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2W (putative)provided by MGI
Primary source: MGI:MGI:1914049
See related: Ensembl:ENSMUSG00000025939 AllianceGenome:MGI:1914049
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 6130401J04Rik
Summary: Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase binding activity. Involved in cellular response to misfolded protein; protein monoubiquitination; and proteolysis involved in protein catabolic process. Located in nucleus. Orthologous to human UBE2W (ubiquitin conjugating enzyme E2 W). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E18 (RPKM 4.9), cortex adult (RPKM 4.9) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 A3; 1 5.1 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (16611012..16689676, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (16540788..16619460, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

[UBE2W overexpression promotes proliferation of intestinal mucosal cells in mice with chemically induced colitis].
Title: [UBE2W overexpression promotes proliferation of intestinal mucosal cells in mice with chemically induced colitis].
UBE2W Improves the Experimental Colitis by Inhibiting the NF-kappaB Signaling Pathway.
Title: UBE2W Improves the Experimental Colitis by Inhibiting the NF-κB Signaling Pathway.
Expression levels of UBE2W in mouse testes were gradually elevated from 2 to 10 weeks, while were significantly deceased in the testes with hypospermatogenesis. When UBE2W expression was successfully down-regulated in spermatogenic cells, the rate of apoptosis was significantly increased and the P53/Bcl-2/caspase 6/caspase 9 signal pathways were activated.
Title: UBE2W down-regulation promotes cell apoptosis and correlates with hypospermatogenesis.
Study shows the absence of ubiquitin conjugating enzyme Ube2W in HdhQ200 knock-in mice increases levels of soluble monomeric mutant huntingtin while reducing insoluble oligomeric species. This study sheds light on the potential function of the non-canonical ubiquitin-conjugating enzyme, Ube2W, in this polyQ neurodegenerative disease.
Title: The ubiquitin conjugating enzyme Ube2W regulates solubility of the Huntington's disease protein, huntingtin.
results establish that UBE2W is an important, albeit not essential, protein for early postnatal survival and normal functioning of multiple organ systems.
Title: Loss of the Ubiquitin-conjugating Enzyme UBE2W Results in Susceptibility to Early Postnatal Lethality and Defects in Skin, Immune, and Male Reproductive Systems.
UBE2W regulates FANCD2 monoubiquitination by mechanisms different from UBE2T and HRR6.
Title: UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
involved_in cellular response to misfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein monoubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein monoubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein monoubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein quality control for misfolded or incompletely synthesized proteins	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 W

Names: E2 ubiquitin-conjugating enzyme W; N-terminal E2 ubiquitin-conjugating enzyme; N-terminus-conjugating E2; probable ubiquitin-conjugating enzyme E2 W; ubiquitin carrier protein W; ubiquitin-protein ligase W

NP_001257945.2

EC 2.3.2.23

EC 2.3.2.25

NP_001257946.2

EC 2.3.2.23

EC 2.3.2.25

NP_080049.3

EC 2.3.2.23

EC 2.3.2.25

XP_006495620.1

EC 2.3.2.23

EC 2.3.2.25

XP_011236704.1

EC 2.3.2.23

EC 2.3.2.25

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271016.3 → NP_001257945.2 ubiquitin-conjugating enzyme E2 W isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site and has alternate 3' exon structure, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct and longer C-terminus, compared to isoform 1.

Source sequence(s)

AC157362, AC162448

Consensus CDS

CCDS87815.1

UniProtKB/TrEMBL

Q3V321, S4R177

Related

ENSMUSP00000138110.3, ENSMUST00000182554.9

Conserved Domains (1) summary

cl00154
Location:7 → 70

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_001271017.2 → NP_001257946.2 ubiquitin-conjugating enzyme E2 W isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (3) has a distinct and shorter C-terminus, compared to isoform 1.

Source sequence(s)

AC162448, BY728312, CN663576

Conserved Domains (1) summary

cl00154
Location:7 → 70

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_025773.4 → NP_080049.3 ubiquitin-conjugating enzyme E2 W isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.

Source sequence(s)

AC162448, AK082233, BY728312

Consensus CDS

CCDS48224.3

UniProtKB/Swiss-Prot

Q8BVJ8, Q8VDW4, Q9D5H3

Related

ENSMUSP00000112741.4, ENSMUST00000117146.9

Conserved Domains (1) summary

pfam00179
Location:7 → 148

UQ_con; Ubiquitin-conjugating enzyme

RNA

NR_073122.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because use of the supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC162448, BY728312, DV044691

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

16611012..16689676 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011238402.4 → XP_011236704.1 ubiquitin-conjugating enzyme E2 W isoform X2

Conserved Domains (1) summary

cl00154
Location:36 → 99

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
XM_006495557.5 → XP_006495620.1 ubiquitin-conjugating enzyme E2 W isoform X1

Conserved Domains (1) summary

pfam00179
Location:36 → 148

UQ_con; Ubiquitin-conjugating enzyme