Snap25 synaptosomal-associated protein 25 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Snap25provided by MGI
Official Full Name: synaptosomal-associated protein 25provided by MGI
Primary source: MGI:MGI:98331
See related: Ensembl:ENSMUSG00000027273 AllianceGenome:MGI:98331
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: sp; Bdr; SUP; GENA70; SNAP-25
Summary: Enables SNAP receptor activity and syntaxin-1 binding activity. Involved in presynaptic dense core vesicle exocytosis and regulation of neuron projection development. Acts upstream of or within several processes, including associative learning; long-term synaptic potentiation; and neurotransmitter secretion. Located in several cellular components, including presynaptic membrane; synaptic vesicle; and trans-Golgi network. Part of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex. Is active in glutamatergic synapse. Is expressed in adrenal gland; jaw; nervous system; and sensory organ. Used to study attention deficit hyperactivity disorder; obesity; schizophrenia; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in Down syndrome and congenital myasthenic syndrome 18. Orthologous to human SNAP25 (synaptosome associated protein 25). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cerebellum adult (RPKM 622.2), cortex adult (RPKM 577.5) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 F3; 2 67.56 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (136555359..136624348)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (136713439..136782428)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SNAP25 disease mutations change the energy landscape for synaptic exocytosis due to aberrant SNARE interactions.
Title: SNAP25 disease mutations change the energy landscape for synaptic exocytosis due to aberrant SNARE interactions.
SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.
Title: SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.
All SNAP25 molecules in the vesicle-plasma membrane contact zone change conformation during vesicle priming.
Title: All SNAP25 molecules in the vesicle-plasma membrane contact zone change conformation during vesicle priming.
SNAP25 is a potential target for early stage Alzheimer's disease and Parkinson's disease.
Title: SNAP25 is a potential target for early stage Alzheimer's disease and Parkinson's disease.
Toward Antifragility: Social Defeat Stress Enhances Learning and Memory in Young Mice Via Hippocampal Synaptosome Associated Protein 25.
Title: Toward Antifragility: Social Defeat Stress Enhances Learning and Memory in Young Mice Via Hippocampal Synaptosome Associated Protein 25.
miR-128 as a Regulator of Synaptic Properties in 5xFAD Mice Hippocampal Neurons.
Title: miR-128 as a Regulator of Synaptic Properties in 5xFAD Mice Hippocampal Neurons.
Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25.
Title: Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25.
Sarcopenia associates with SNAP-25 SNPs and a miRNAs profile which is modulated by structured rehabilitation treatment.
Title: Sarcopenia associates with SNAP-25 SNPs and a miRNAs profile which is modulated by structured rehabilitation treatment.
Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.
Title: Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.
miR-128 regulates epilepsy sensitivity in mice by suppressing SNAP-25 and SYT1 expression in the hippocampus.
Title: miR-128 regulates epilepsy sensitivity in mice by suppressing SNAP-25 and SYT1 expression in the hippocampus.

Submit: New GeneRIF Correction See all GeneRIFs (100)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2) 1 citation
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127050 (e-PCR)
- UniSTS:207407

D3Wox20 (e-PCR)
- UniSTS:118108

D2Nds33 (e-PCR), detects polymorphism
- UniSTS:118108

D25 (e-PCR)
- UniSTS:129162

D3M2Mit28 (e-PCR)
- UniSTS:129162

D2Mit28.1 (e-PCR), detects polymorphism
- UniSTS:129163

D2Mit28.3 (e-PCR), detects polymorphism
- UniSTS:129164

D3Kyo3 (e-PCR)
- UniSTS:240323

Snap25 (e-PCR), detects polymorphism
- UniSTS:532437

MARC_11717-11718:997472340:1 (e-PCR)
- UniSTS:267259

MARC_11717-11718:1029180244:1 (e-PCR)
- UniSTS:267259

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNAP receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables SNARE binding	IDA Inferred from Direct Assay more info	PubMed
enables SNARE binding	ISO Inferred from Sequence Orthology more info	PubMed
enables calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables myosin binding	IEA Inferred from Electronic Annotation more info
enables myosin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables syntaxin-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-1 binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin-1 binding	ISO Inferred from Sequence Orthology more info	PubMed
enables transmembrane transporter binding	IEA Inferred from Electronic Annotation more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info
enables voltage-gated potassium channel activity	IEA Inferred from Electronic Annotation more info
enables voltage-gated potassium channel activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SNARE complex assembly	IEA Inferred from Electronic Annotation more info
involved_in SNARE complex assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within associative learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonogenesis	IEA Inferred from Electronic Annotation more info
involved_in calcium ion-regulated exocytosis of neurotransmitter	ISO Inferred from Sequence Orthology more info
involved_in endosomal transport	IEA Inferred from Electronic Annotation more info
involved_in endosomal transport	ISO Inferred from Sequence Orthology more info
involved_in establishment of localization in cell	ISO Inferred from Sequence Orthology more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in insulin secretion	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term memory	IEA Inferred from Electronic Annotation more info
involved_in long-term memory	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neurotransmitter secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of hormone secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in presynaptic dense core vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic dense core vesicle exocytosis	IEP Inferred from Expression Pattern more info	PubMed
involved_in presynaptic dense core vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of establishment of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron projection development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in regulation of synapse assembly	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle fusion to presynaptic active zone membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle priming	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of BLOC-1 complex	ISO Inferred from Sequence Orthology more info
part_of BLOC-1 complex	ISS Inferred from Sequence or Structural Similarity more info
part_of SNARE complex	ISO Inferred from Sequence Orthology more info
part_of SNARE complex	ISS Inferred from Sequence or Structural Similarity more info
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info	PubMed
located_in axonal growth cone	ISO Inferred from Sequence Orthology more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in extrinsic component of presynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in extrinsic component of presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in filopodium	IEA Inferred from Electronic Annotation more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in growth cone	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	HDA more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in photoreceptor inner segment	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in presynaptic active zone membrane	ISO Inferred from Sequence Orthology more info
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in ribbon synapse	IDA Inferred from Direct Assay more info	PubMed
located_in somatodendritic compartment	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	IEA Inferred from Electronic Annotation more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	ISO Inferred from Sequence Orthology more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with vesicle	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: synaptosomal-associated protein 25

Names: blind drunk; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein, 25 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001291056.2 → NP_001277985.1 synaptosomal-associated protein 25 isoform b

See identical proteins and their annotated locations for NP_001277985.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon but contains a different in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is the same length but contains an alternate internal segment, compared to isoform a.

Source sequence(s)

AL732447

Consensus CDS

CCDS71153.1

UniProtKB/Swiss-Prot

P60879

Related

ENSMUSP00000105725.4, ENSMUST00000110098.4

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family
NM_001355254.1 → NP_001342183.1 synaptosomal-associated protein 25 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 both encode the same isoform (a).

Source sequence(s)

AL732447

Consensus CDS

CCDS16793.1

UniProtKB/Swiss-Prot

A2AIC2, A2AIC3, P60879

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family
NM_001355255.2 → NP_001342184.1 synaptosomal-associated protein 25 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses an alternate in-frame splice junction and lacks an in-frame exon but contains a different in-frame exon in the central coding region, compared to variant 1. The encoded isoform (d) is the same length but contains an alternate internal segment, compared to isoform a.

Source sequence(s)

AL732447

Conserved Domains (3) summary

cd15885
Location:139 → 197

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 137

SNAP-25; SNAP-25 family
NM_001412615.1 → NP_001399544.1 synaptosomal-associated protein 25 isoform a

Status: VALIDATED

Source sequence(s)

AL732447

UniProtKB/Swiss-Prot

A2AIC2, A2AIC3, P60879
NM_001412616.1 → NP_001399545.1 synaptosomal-associated protein 25 isoform a

Status: VALIDATED

Source sequence(s)

AL732447

UniProtKB/Swiss-Prot

A2AIC2, A2AIC3, P60879
NM_001412617.1 → NP_001399546.1 synaptosomal-associated protein 25 isoform b

Status: VALIDATED

Source sequence(s)

AL732447
NM_001412618.1 → NP_001399547.1 synaptosomal-associated protein 25 isoform c

Status: VALIDATED

Source sequence(s)

AL732447
NM_001412619.1 → NP_001399548.1 synaptosomal-associated protein 25 isoform d

Status: VALIDATED

Source sequence(s)

AL732447
NM_001412620.1 → NP_001399549.1 synaptosomal-associated protein 25 isoform g

Status: VALIDATED

Source sequence(s)

AL732447
NM_001412621.1 → NP_001399550.1 synaptosomal-associated protein 25 isoform e

Status: VALIDATED

Source sequence(s)

AL732447
NM_001412622.1 → NP_001399551.1 synaptosomal-associated protein 25 isoform f

Status: VALIDATED

Source sequence(s)

AL732447
NM_011428.4 → NP_035558.1 synaptosomal-associated protein 25 isoform a

See identical proteins and their annotated locations for NP_035558.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform a. Variants 1 and 3 both encode the same isoform (a).

Source sequence(s)

AL732447

Consensus CDS

CCDS16793.1

UniProtKB/Swiss-Prot

A2AIC2, A2AIC3, P60879

Related

ENSMUSP00000028727.5, ENSMUST00000028727.11

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family

RNA

NR_149318.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (5) uses an alternate splice junction compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AL732447
NR_182008.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL732447
NR_182009.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL732447

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

136555359..136624348

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316761.2 → XP_017172250.1 synaptosomal-associated protein 25 isoform X2

Conserved Domains (3) summary

cd15885
Location:80 → 138

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

pfam00835
Location:28 → 78

SNAP-25; SNAP-25 family

cl22856
Location:1 → 19

SNARE; SNARE motif