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    sspB ClpXP protease specificity-enhancing factor [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944774, updated on 3-Dec-2024

    Summary

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    Official Symbol
    sspB
    Official Full Name
    ClpXP protease specificity-enhancing factor
    Primary source
    ECOCYC:EG10978
    Locus tag
    b3228
    See related
    ASAP:ABE-0010591
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3217
    Summary
    The SspB protein is a specificity-enhancing factor for the ClpXP protease . [More information is available at EcoCyc: EG10978].
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    Genomic context

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    Sequence:
    NC_000913.3 (3376279..3376776, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NanR Neighboring gene putative transporter DcuD Neighboring gene stringent starvation protein A Neighboring gene 30S ribosomal subunit protein S9

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The SspB adaptor drives structural changes in the AAA+ ClpXP protease during ssrA-tagged substrate delivery. Ghanbarpour A, et al. Proc Natl Acad Sci U S A, 2023 Feb 7. PMID 36730206, Free PMC Article
    2. Survival of Anaerobic Fe(2+) Stress Requires the ClpXP Protease. Bennett BD, et al. J Bacteriol, 2018 Apr 15. PMID 29378887, Free PMC Article
    3. Small-molecule control of protein degradation using split adaptors. Davis JH, et al. ACS Chem Biol, 2011 Nov 18. PMID 21866931, Free PMC Article
    4. Versatile modes of peptide recognition by the AAA+ adaptor protein SspB. Levchenko I, et al. Nat Struct Mol Biol, 2005 Jun. PMID 15880122
    5. Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery. McGinness KE, et al. J Biol Chem, 2007 Apr 13. PMID 17317664

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The SspB adaptor drives structural changes in the AAA+ ClpXP protease during ssrA-tagged substrate delivery.
      Title: The SspB adaptor drives structural changes in the AAA+ ClpXP protease during ssrA-tagged substrate delivery.
    2. Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates.
      Title: Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates.
    3. Here we demonstrate a role for ClpXP, a protease system widely conserved in bacteria, in anaerobic Fe(2+) resistance in both S. oneidensis and Escherichia coli
      Title: Survival of Anaerobic Fe(2+) Stress Requires the ClpXP Protease.
    4. The describe an engineered split-adaptor system, in which sspB protein assembly and delivery of substrates to the ClpXP protease depends on a small molecule (rapamycin).
      Title: Small-molecule control of protein degradation using split adaptors.
    5. results also reveal highly conserved residues in the ClpX-binding peptides of both E. coli SspB and C. crescentus SspBalpha that play no detectable role in ClpX-binding or substrate delivery.
      Title: Versatile modes of peptide recognition by the ClpX N domain mediate alternative adaptor-binding specificities in different bacterial species.
    6. ClpXP, together with SspB, is uniquely adapted for degradation of SsrA-tagged proteins and is responsible for the major part of their degradation in vivo
      Title: Turnover of endogenous SsrA-tagged proteins mediated by ATP-dependent proteases in Escherichia coli.
    7. N-terminus verified by Edman degradation on complete protein
      Title: A specificity-enhancing factor for the ClpXP degradation machine.
    8. properties of the degradation tag and the number of SspB.ClpX tethering interactions are the major factors that determine the extent to which the substrate and ClpX are engaged in ternary delivery complexes
      Title: Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATPase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables RNA binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables molecular adaptor activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in positive regulation of ATP-dependent activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within positive regulation of protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of proteolysis involved in protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of HslUV protease complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ClpXP protease specificity-enhancing factor

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3376279..3376776 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417695.1 ClpXP protease specificity-enhancing factor [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417695.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1EXQ3
      Conserved Domains (1) summary
      COG2969
      Location:2 → 133
      SspB; Stringent starvation protein B, binds SsrA peptide [Posttranslational modification, protein turnover, chaperones]
    Nucleotide Protein
    Heading Accession and Version

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