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    ptsN phosphotransferase system enzyme IIA(Ntr) [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947721, updated on 17-Dec-2024

    Summary

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    Official Symbol
    ptsN
    Official Full Name
    phosphotransferase system enzyme IIA(Ntr)
    Primary source
    ECOCYC:EG11682
    Locus tag
    b3204
    See related
    ASAP:ABE-0010520
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3193; rpoP; yhbI
    Summary
    Although ptsN was predicted to have a sigma 28 (FliA) promoter, it can not be transcribed in vitro with sigma 28 holoenzyme (Yu, 2006). [More information is available at EcoGene: EG11682]. ptsN encodes a protein homologous to Enzyme IIAfru of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS). [More information is available at EcoCyc: EG11682].
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    Genomic context

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    Sequence:
    NC_000913.3 (3346578..3347069)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNA polymerase sigma factor RpoN Neighboring gene ribosome hibernation-promoting factor Neighboring gene RNase adaptor protein RapZ Neighboring gene phosphorelay protein NPr Neighboring gene protein kinase-like domain-containing protein YrbL Neighboring gene peptidoglycan glycosyltransferase MtgA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control. Gravina F, et al. Res Microbiol, 2021 Nov-Dec. PMID 34563668
    2. Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli. Lee CR, et al. Mol Microbiol, 2013 May. PMID 23517463, Free PMC Article
    3. 1H, 15N, and 13C chemical shift assignments of the Escherichia coli nitrogen regulatory phosphocarrier IIA(Ntr). Li X, et al. J Biomol NMR, 2003 Dec. PMID 14512741
    4. The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli. Bordo D, et al. J Mol Biol, 1998 May 29. PMID 9636714
    5. Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes. Gravina F, et al. J Proteomics, 2018 Mar 1. PMID 29274402

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
      Title: The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
    2. ptsN is required for fitness under minimal medium and for the proper balance between RpoS and sigma 70 activities in such way that, in the absence of ptsN, RpoS-dependent genes are preferentially expressed.
      Title: Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
    3. Overproduction of YcgO yields a potassium-sensitive phenotype with similarities to that displayed by the DeltaptsN mutation.
      Title: Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO.
    4. Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation.
      Title: Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.
    5. Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG.
      Title: Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG.
    6. PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level.
      Title: Potassium mediates Escherichia coli enzyme IIA(Ntr) -dependent regulation of sigma factor selectivity.
    7. Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD.
      Title: Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli.
    8. EIIANtr is readily phosphorylated at the histidine 73.
      Title: Requirements for the phosphorylation of the Escherichia coli EIIANtr protein in vivo.
    9. overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E).
      Title: The extracytoplasmic stress factor, sigmaE, is required to maintain cell envelope integrity in Escherichia coli.
    10. The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy.
      Title: NMR characterization of the Escherichia coli nitrogen regulatory protein IIANtr in solution and interaction with its partner protein, NPr.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables enzyme inhibitor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein kinase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-N(PI)-phosphohistidine-sugar phosphotransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in phosphoenolpyruvate-dependent sugar phosphotransferase system IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    phosphotransferase system enzyme IIA(Ntr)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3346578..3347069
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417671.1 phosphotransferase system enzyme IIA(Ntr) [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417671.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3I349, A0A1X3IXV1, A0A1X3JDA0, A0AAJ3U1G2
      Conserved Domains (1) summary
      PRK10896
      Location:2 → 155
      PRK10896; PTS IIA-like nitrogen regulatory protein PtsN
    Nucleotide Protein
    Heading Accession and Version

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