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    umuC DNA polymerase V catalytic protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946359, updated on 3-Dec-2024

    Summary

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    Official Symbol
    umuC
    Official Full Name
    DNA polymerase V catalytic protein
    Primary source
    ECOCYC:EG11056
    Locus tag
    b1184
    See related
    ASAP:ABE-0003977
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1172; uvm
    Summary
    ATPgammaS inhibits UmuC activity. [More information is available at EcoGene: EG11056]. The chaperones Hsp70 (DnaK) and Hsp60 (MopA) help fold UmuC into its functional form . [More information is available at EcoCyc: EG11056].
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    Genomic context

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    Sequence:
    NC_000913.3 (1231186..1232454)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hemolysin E Neighboring gene DNA polymerase V protein UmuD Neighboring gene protein thiol:quinone oxidoreductase DsbB Neighboring gene Na(+):H(+) antiporter NhaB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Critical amino acids in Escherichia coli UmuC responsible for sugar discrimination and base-substitution fidelity. Vaisman A, et al. Nucleic Acids Res, 2012 Jul. PMID 22422840, Free PMC Article
    2. An aerobic recA-, umuC-dependent pathway of spontaneous base-pair substitution mutagenesis in Escherichia coli. Bhamre S, et al. Mutat Res, 2001 Feb 20. PMID 11166040
    3. Sequential folding of UmuC by the Hsp70 and Hsp60 chaperone complexes of Escherichia coli. Petit MA, et al. J Biol Chem, 1994 Sep 23. PMID 7916347
    4. Steric gate variants of UmuC confer UV hypersensitivity on Escherichia coli. Shurtleff BW, et al. J Bacteriol, 2009 Aug. PMID 19482923, Free PMC Article
    5. Homology modeling of four Y-family, lesion-bypass DNA polymerases: the case that E. coli Pol IV and human Pol kappa are orthologs, and E. coli Pol V and human Pol eta are orthologs. Lee CH, et al. J Mol Graph Model, 2006 Sep. PMID 16386932

    See all (149) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Tracking Escherichia coli DNA polymerase V to the entire genome during the SOS response.
      Title: Tracking Escherichia coli DNA polymerase V to the entire genome during the SOS response.
    2. A Comprehensive View of Translesion Synthesis in Escherichia coli.
      Title: A Comprehensive View of Translesion Synthesis in Escherichia coli.
    3. We found that the UmuC C-terminal extension has a short region homologous with the RecA N-terminal fragment, itself involved in RecA filament formation. Based on this observation, we proposed a structural model for the UmuC-RecA interaction that rationalizes prior experimental observations.
      Title: New insights into the structures and interactions of bacterial Y-family DNA polymerases.
    4. DNA binding and synthesis cannot occur until pol V interacts with a RecA nucleoprotein filament (RecA*) and ATP to form a mutasome complex, pol V Mut = UmuD'2C-RecA-ATP.
      Title: Conformational regulation of Escherichia coli DNA polymerase V by RecA and ATP.
    5. This work reveals a new level of regulation in the activation of DNA polymerase V. pol V is subject to a novel form of spatial regulation.
      Title: Regulation of Mutagenic DNA Polymerase V Activation in Space and Time.
    6. The UmuC peptide follows the overall disposition of previously characterised structures with respect to the highly conserved glutamine residue.
      Title: The UmuC subunit of the E. coli DNA polymerase V shows a unique interaction with the β-clamp processivity factor.
    7. Y11A UmuC mutant has increased UV-sensitivity and reduced UV-mutability due to excessive incorporation of rNTPs during translesion DNA synthesis.
      Title: Escherichia coli UmuC active site mutants: effects on translesion DNA synthesis, mutagenesis and cell survival.
    8. Variants of UmuC with amino acid substitutions at, or adjacent to, its steric gate were studied to gain insights into sugar selectivity and overall fidelity of E. coli pol V.
      Title: Critical amino acids in Escherichia coli UmuC responsible for sugar discrimination and base-substitution fidelity.
    9. Polymerases II, IV and V may bypass trans-4-hydroxy-2-nonenal modified bases by translesion synthesis and restore DNA synthesis beyond the arrested site, although with different efficiencies.
      Title: Role of damage-specific DNA polymerases in M13 phage mutagenesis induced by a major lipid peroxidation product trans-4-hydroxy-2-nonenal.
    10. REVIEW: properties and functions of Pol V
      Title: Properties and functions of Escherichia coli: Pol IV and Pol V.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP-dependent activity, acting on DNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables damaged DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in SOS response IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in SOS response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in SOS response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in SOS response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in error-prone translesion synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translesion synthesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA polymerase V complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of DNA polymerase V complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytosol IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase V catalytic protein
    NP_415702.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1231186..1232454
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415702.1 DNA polymerase V catalytic protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415702.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6BBY9
      Conserved Domains (1) summary
      PRK03609
      Location:1 → 422
      umuC; translesion error-prone DNA polymerase V subunit UmuC
    Nucleotide Protein
    Heading Accession and Version

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