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    rnt ribonuclease T [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946159, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rnt
    Official Full Name
    ribonuclease T
    Primary source
    ECOCYC:EG11547
    Locus tag
    b1652
    See related
    ASAP:ABE-0005523
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1648
    Summary
    RNase T is a 3'-5' exonuclease responsible for 3' trimming of many stable RNAs, including tRNAs. [More information is available at EcoCyc: EG11547].
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    Genomic context

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    Sequence:
    NC_000913.3 (1728347..1728994)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene N-ethylmaleimide reductase Neighboring gene lactoylglutathione lyase Neighboring gene ATP-dependent helicase Lhr Neighboring gene glutaredoxin 4

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Aromatic residues in RNase T stack with nucleobases to guide the sequence-specific recognition and cleavage of nucleic acids. Duh Y, et al. Protein Sci, 2015 Dec. PMID 26362012, Free PMC Article
    2. Escherichia coli RNase T functions in vivo as a dimer dependent on cysteine 168. Li Z, et al. J Biol Chem, 1996 Jan 12. PMID 8557641
    3. Ribonuclease T: new exoribonuclease possibly involved in end-turnover of tRNA. Deutscher MP, et al. Proc Natl Acad Sci U S A, 1984 Jul. PMID 6379642, Free PMC Article
    4. The DNase activity of RNase T and its application to DNA cloning. Zuo Y, et al. Nucleic Acids Res, 1999 Oct 15. PMID 10497273, Free PMC Article
    5. Purification and characterization of Escherichia coli RNase T. Deutscher MP, et al. J Biol Chem, 1985 Jun 10. PMID 3888994

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. e utilize Escherichia coli RNase T, a tRNA-processing enzyme responsible for the tRNA-CCA-end turnover, to generate an in vivo system for the evaluation of A-adding activity.
      Title: Dual expression of CCA-adding enzyme and RNase T in Escherichia coli generates a distinct cca growth phenotype with diverse applications.
    2. Results provide evidence showing that the pi-pi stacking interactions between nucleobases and protein aromatic residues may guide the sequence-specific activity for RNase T.
      Title: Aromatic residues in RNase T stack with nucleobases to guide the sequence-specific recognition and cleavage of nucleic acids.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' exonuclease activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables 3'-5' exonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-5' exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3'-5'-RNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA exonuclease activity, producing 5'-phosphomonoesters IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA nuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA 3'-5' DNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication proofreading IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to UV EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in rRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulatory ncRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA 3'-end processing EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in tRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribonuclease T

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1728347..1728994
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416169.1 ribonuclease T [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416169.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376S8X1, A0A5B9AIC0
      Conserved Domains (1) summary
      PRK05168
      Location:1 → 211
      PRK05168; ribonuclease T; Provisional
    Nucleotide Protein
    Heading Accession and Version

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