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    ligA DNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946885, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ligA
    Official Full Name
    DNA ligase
    Primary source
    ECOCYC:EG10534
    Locus tag
    b2411
    See related
    ASAP:ABE-0007938
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dnaL; ECK2406; lig; lop; pdeC
    Summary
    ligA is an essential gene. [More information is available at EcoGene: EG10534]. LigA is one of two known NAD(+)-dependent DNA ligases, catalyzing the formation of phosphodiester bonds in duplex DNA. [More information is available at EcoCyc: EG10534].
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    Genomic context

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    Sequence:
    NC_000913.3 (2528161..2530176, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator YfeR Neighboring gene putative solute:Na(+) symporter Neighboring gene PF12843 family protein YpeB Neighboring gene cell division protein ZipA Neighboring gene sulfate:H(+) symporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Suppression of a mutation in gene 3 of bacteriophage T7 (T7 endonuclease I) by mutations in phage and host polynucleotide ligase. Sadowski PD. J Virol, 1974 Jan. PMID 4590018, Free PMC Article
    2. Enzymatic joining of DNA strands, II. An enzyme-adenylate intermediate in the dpn-dependent DNA ligase reaction. Little JW, et al. Proc Natl Acad Sci U S A, 1967 Nov. PMID 4295585, Free PMC Article
    3. Joining of DNA strands by DNA ligase of E. coli. Gellert M, et al. Cold Spring Harb Symp Quant Biol, 1968. PMID 4306814
    4. Some observations on the cofactor requirement for partially purified DNA ligase from Escherichia coli. Feldberg RS, et al. FEBS Lett, 1972 Nov 1. PMID 4352028
    5. Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase. Konrad EB, et al. J Mol Biol, 1973 Jul 15. PMID 4353283

    See all (83) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA).
      Title: Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA).
    2. Ensemble and single-molecule experiments reveal that phage Mu Gam protein binds to DNA ends, slows the progress of RecBCD exonuclease, promotes binding of NAD(+)-dependent ligase A, and stimulates ligation.
      Title: Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligase.
    3. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described.
      Title: Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA).
    4. Here the authors show that replication without ligase kills after a short resistance period.
      Title: Chromosome demise in the wake of ligase-deficient replication.
    5. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation
      Title: Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.
    6. N-terminus verified by Edman degradation on complete protein
      Title: Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.
    7. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding.
      Title: Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA).
    8. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition
      Title: Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.
    9. temperature sensitive mutant characterized
      Title: Characterization of a temperature-sensitive DNA ligase from Escherichia coli.
    10. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA)
      Title: Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA).
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA ligase (NAD+) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA ligase (NAD+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA ligase (NAD+) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD+ binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA ligation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair, DNA ligation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within base-excision repair, DNA ligation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA ligase
    NP_416906.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2528161..2530176 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416906.1 DNA ligase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416906.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN3NUD9
      Conserved Domains (1) summary
      PRK07956
      Location:1 → 670
      ligA; NAD-dependent DNA ligase LigA; Validated
    Nucleotide Protein
    Heading Accession and Version

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