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    pyrG CTP synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946116, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pyrG
    Official Full Name
    CTP synthetase
    Primary source
    ECOCYC:EG10810
    Locus tag
    b2780
    See related
    ASAP:ABE-0009112
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2774
    Summary
    PyrG uses either glutamine or ammonia as the nitrogen source. [More information is available at EcoGene: EG10810]. CTP synthetase catalyzes the glutamine- or ammonia-dependent synthesis of CTP from UTP, the final step in the de novo biosynthesis of CTP. [More information is available at EcoCyc: EG10810].
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    Genomic context

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    Sequence:
    NC_000913.3 (2908029..2909666, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene TPM domain-containing protein YgcG Neighboring gene enolase Neighboring gene nucleoside triphosphate pyrophosphohydrolase Neighboring gene endoribonuclease toxin MazF Neighboring gene antitoxin of the MazF-MazE toxin-antitoxin system MazE

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. CTP synthetase from Escherichia coli: an improved purification procedure and characterization of hysteretic and enzyme concentration effects on kinetic properties. Anderson PM. Biochemistry, 1983 Jun 21. PMID 6349684
    2. Role of glutamine in the biosynthesis of cytidine nucleotides in Escherichia coli. CHAKRABORTY KP, et al. Biochim Biophys Acta, 1961 Mar 4. PMID 13692169
    3. Enzymatic amination of uridine triphosphate to cytidine triphosphate. LIEBERMAN I. J Biol Chem, 1956 Oct. PMID 13367044
    4. Limited proteolysis of Escherichia coli cytidine 5'-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis. Simard D, et al. Eur J Biochem, 2003 May. PMID 12752439
    5. Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP synthase. Iyengar A, et al. Biochem J, 2003 Feb 1. PMID 12383057, Free PMC Article

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We also performed biochemical assays to monitor template DNA translocation during RNA synthesis from the pyrG promoter and in vitro transcription assays to determine the length of poly-G RNA from the pyrG promoter variants.
      Title: Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    3. The conformational change associated with binding ATP may be transmitted through the L10-alpha11 structural unit (residues 297-312) and thereby mediate effects on the glutaminase activity of CTPS.
      Title: The role of lysine residues 297 and 306 in nucleoside triphosphate regulation of E. coli CTP synthase: inactivation by 2',3'-dialdehyde ATP and mutational analyses.
    4. results depict a novel product inhibition strategy in which shared substrate & product moieties bind to a single subsite while specificity is conferred by separate subsites; this allows for independent adaptation of UTP & CTP binding affinities
      Title: Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables CTP synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables CTP synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables CTP synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glutaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in 'de novo' CTP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in CTP biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within CTP biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in CTP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within CTP biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in pyrimidine nucleobase biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pyrimidine nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoophidium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    CTP synthetase
    NP_417260.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2908029..2909666 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417260.1 CTP synthetase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417260.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1PEF8
      Conserved Domains (1) summary
      PRK05380
      Location:3 → 543
      pyrG; CTP synthetase; Validated
    Nucleotide Protein
    Heading Accession and Version

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