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    rraA ribonuclease E inhibitor protein A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948419, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rraA
    Official Full Name
    ribonuclease E inhibitor protein A
    Primary source
    ECOCYC:EG11879
    Locus tag
    b3929
    See related
    ASAP:ABE-0012837
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3921; menG; yiiV
    Summary
    rraA (menG) was previously, erroneously, reported to encode demethylmenaquinone methyltransferase, in Genbank U56082. [More information is available at EcoGene: EG11879]. RraA inhibits ribonuclease E (RNase E, Rne) activity. [More information is available at EcoCyc: EG11879].
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    Genomic context

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    Sequence:
    NC_000913.3 (4118845..4119330, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene glycerol facilitator Neighboring gene cell division factor ZapB Neighboring gene 1,4-dihydroxy-2-naphthoate octaprenyltransferase Neighboring gene ATPase component of the HslVU protease

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Roles of the 5'-phosphate sensor domain in RNase E. Garrey SM, et al. Mol Microbiol, 2011 Jun. PMID 21518390
    2. Effects of Escherichia coli RraA orthologs of Vibrio vulnificus on the ribonucleolytic activity of RNase E in vivo. Lee M, et al. Curr Microbiol, 2009 Apr. PMID 19194749
    3. Ribonuclease PH interacts with an acidic ribonuclease E site through a basic 80-amino acid domain. Martínez VP, et al. FEMS Microbiol Lett, 2014 Jun. PMID 24766456
    4. Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases. Pietras Z, et al. J Biol Chem, 2013 Nov 1. PMID 24045937, Free PMC Article
    5. The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome. Górna MW, et al. RNA, 2010 Mar. PMID 20106955, Free PMC Article

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PNPase and RNase PH facilitate the stability and regulatory function of the sRNAs RyhB, CyaR, and MicA during exponential growth.
      Title: The Phosphorolytic Exoribonucleases Polynucleotide Phosphorylase and RNase PH Stabilize sRNAs and Facilitate Regulation of Their mRNA Targets.
    2. Authors narrowed down the sites of interaction in each enzyme to an acidic 16-amino acid region in the carboxy-terminal domain of RNase E and a basic 80-amino acid region in RNase PH including an alpha3 helix
      Title: Ribonuclease PH interacts with an acidic ribonuclease E site through a basic 80-amino acid domain.
    3. The phosphate sensor is required for efficient autoregulation of RNase E synthesis.
      Title: Roles of the 5'-phosphate sensor domain in RNase E.
    4. The truncated RNase E750 can still bind to Hfq although the truncation significantly reduces the Hfq-binding ability.
      Title: Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coli.
    5. Data suggest that RraA can affect multiple components of the RNA degradosome in a dynamic, energy-dependent equilibrium.
      Title: The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome.
    6. RraA and RraB have a conserved property to directly act on RNAse E-related enzymes and inhibit their ribonucleolytic activity.
      Title: Inhibitory effects of RraA and RraB on RNAse E-related enzymes imply conserved functions in the regulated enzymatic cleavage of RNA.
    7. expression of RraA or RraB is accompanied by specific changes in degradosome composition--and that these are in turn associated with alterations in RNA decay
      Title: Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome.
    8. Findings suggest RraA is able to effectively modulate the catalytic activity of RNase E in vivo.
      Title: RraA rescues Escherichia coli cells over-producing RNase E from growth arrest by modulating the ribonucleolytic activity.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables endoribonuclease inhibitor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ribonuclease inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribonuclease inhibitor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of RNA catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within negative regulation of RNA catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein homotrimerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of RNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribonuclease E inhibitor protein A

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4118845..4119330 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418364.1 ribonuclease E inhibitor protein A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418364.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AR25
      Conserved Domains (1) summary
      PRK09372
      Location:1 → 159
      PRK09372; ribonuclease E inhibitor RraA
    Nucleotide Protein
    Heading Accession and Version

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