SWA2 auxilin-like protein SWA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SWA2
Official Full Name: auxilin-like protein SWA2
Primary source: SGD:S000002728
Locus tag: YDR320C
See related: AllianceGenome:SGD:S000002728; FungiDB:YDR320C; VEuPathDB:YDR320C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AUX1; BUD24
Summary: Enables clathrin binding activity and ubiquitin binding activity. Involved in clathrin coat disassembly and endoplasmic reticulum inheritance. Located in endoplasmic reticulum membrane. Human ortholog(s) of this gene implicated in Parkinson's disease and Parkinson's disease 19A. Orthologous to human DNAJC6 (DnaJ heat shock protein family (Hsp40) member C6). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SWA2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1106095..1108101, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These investigations suggest that Hsp90 may be the critical binding partner of Swa2 in [URE3] propagation, and we propose a new model for plausible Swa2-Hsp90 cooperation in [URE3] prion propagation.
Title: Prion-specific Hsp40 function: The role of the auxilin homolog Swa2.
study reports novel role for Swa2/Aux1; found that, in addition to Sis1,[URE3-1] is specifically dependent upon Swa2; data suggest that prion-chaperone specificity arises from the Swa2 TPR domain and supports a model where Swa2 acts through Hsp70, most likely to provide additional access points for Hsp104 to promote prion template generation
Title: Swa2, the yeast homolog of mammalian auxilin, is specifically required for the propagation of the prion variant [URE3-1].
Ssa1p and Swa2p cooperatively disassemble yeast clathrin coat baskets
Title: Clathrin coat disassembly by the yeast Hsc70/Ssa1p and auxilin/Swa2p proteins observed by single-particle burst analysis spectroscopy.
Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing.
Title: Swa2p-dependent clathrin dynamics is critical for Flo11p processing and 'Mat' formation in the yeast Saccharomyces cerevisiae.
Ubiquitin (Ub) recognition occurs predominantly through an atypical interaction in which the ubiquitin-associated (UBA) helix alpha1 and the N-terminal part of helix alpha2 of Swa2 bind to Ub.
Title: Atypical binding of the Swa2p UBA domain to ubiquitin.
These data suggest that the TPR and J-domains of SWA2 collaborate in a bipartite interaction with Hsp70 to regulate its activity in clathrin disassembly.
Title: Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables clathrin binding	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in clathrin coat disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin coat disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in clathrin coat disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in vesicle	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: auxilin-like protein SWA2

NP_010606.1

Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles; required for propagation of [URE3] prion variant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1106095..1108101 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180628.1 → NP_010606.1 TPA: auxilin-like protein SWA2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010606.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSV1, Q06677

UniProtKB/TrEMBL

A6ZYS1, B3LFX8, N1P6W8

Conserved Domains (3) summary

COG0457
Location:374 → 497

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:374 → 402

TPR; TPR repeat [structural motif]

pfam09145
Location:138 → 181

Ubiq-assoc; Ubiquitin-associated