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    Nt5e 5' nucleotidase, ecto [ Mus musculus (house mouse) ]

    Gene ID: 23959, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Nt5eprovided by MGI
    Official Full Name
    5' nucleotidase, ectoprovided by MGI
    Primary source
    MGI:MGI:99782
    See related
    Ensembl:ENSMUSG00000032420 AllianceGenome:MGI:99782
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    NT; Nt5; eNT; CD73; 5'-NT; 2210401F01Rik
    Summary
    This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]
    Expression
    Broad expression in bladder adult (RPKM 18.6), lung adult (RPKM 11.8) and 21 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Nt5e in Genome Data Viewer
    Location:
    9 E3.1; 9 47.24 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (88209662..88254142)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (88327609..88372089)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene solute carrier family 25, member 40 pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E8304 Neighboring gene STARR-seq mESC enhancer starr_24855 Neighboring gene microRNA 12205 Neighboring gene sorting nexin 14 Neighboring gene STARR-positive B cell enhancer ABC_E10573 Neighboring gene 60S ribosomal protein L21 pseudogene Neighboring gene STARR-seq mESC enhancer starr_24856 Neighboring gene synaptotagmin binding, cytoplasmic RNA interacting protein Neighboring gene STARR-positive B cell enhancer ABC_E9772

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Extracellular adenosine produced by ecto-5'-nucleotidase (CD73) regulates macrophage pro-inflammatory responses, nitric oxide production, and favors Salmonella persistence. Costales MG, et al. Nitric Oxide, 2018 Jan 30. PMID 29108754
    2. CD73-generated adenosine facilitates Toxoplasma gondii differentiation to long-lived tissue cysts in the central nervous system. Mahamed DA, et al. Proc Natl Acad Sci U S A, 2012 Oct 2. PMID 22988118, Free PMC Article
    3. Lack of ecto-5'-nucleotidase (CD73) promotes arteriogenesis. Böring YC, et al. Cardiovasc Res, 2013 Jan 1. PMID 22977005
    4. Adenosine Formed by CD73 on T Cells Inhibits Cardiac Inflammation and Fibrosis and Preserves Contractile Function in Transverse Aortic Constriction-Induced Heart Failure. Quast C, et al. Circ Heart Fail, 2017 Apr. PMID 28404626
    5. Inhibition of CD73 improves B cell-mediated anti-tumor immunity in a mouse model of melanoma. Forte G, et al. J Immunol, 2012 Sep 1. PMID 22826317, Free PMC Article

    See all (247) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Intrinsic ecto-5'-Nucleotidase/A1R Coupling may Confer Neuroprotection to the Cerebellum in Experimental Autoimmune Encephalomyelitis.
      Title: Intrinsic ecto-5'-Nucleotidase/A(1)R Coupling may Confer Neuroprotection to the Cerebellum in Experimental Autoimmune Encephalomyelitis.
    2. CD73 induces GM-CSF/MDSC-mediated suppression of T cells to accelerate pancreatic cancer pathogenesis.
      Title: CD73 induces GM-CSF/MDSC-mediated suppression of T cells to accelerate pancreatic cancer pathogenesis.
    3. Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy through negative feedback regulation of the NF-kappaB pathway.
      Title: Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy through negative feedback regulation of the NF-κB pathway.
    4. Enhanced immunosuppressive capability of mesenchymal stem cell-derived small extracellular vesicles with high expression of CD73 in experimental autoimmune uveitis.
      Title: Enhanced immunosuppressive capability of mesenchymal stem cell-derived small extracellular vesicles with high expression of CD73 in experimental autoimmune uveitis.
    5. Cdc73 protects Notch-induced T-cell leukemia cells from DNA damage and mitochondrial stress.
      Title: Cdc73 protects Notch-induced T-cell leukemia cells from DNA damage and mitochondrial stress.
    6. Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
      Title: Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.
    7. CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner.
      Title: CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner.
    8. CD73-dependent generation of extracellular adenosine by vascular endothelial cells modulates de novo lipogenesis in adipose tissue.
      Title: CD73-dependent generation of extracellular adenosine by vascular endothelial cells modulates de novo lipogenesis in adipose tissue.
    9. Role of ecto-5'-nucleotidase in bladder function.
      Title: Role of ecto-5'-nucleotidase in bladder function.
    10. CD73 Blockade Alleviated Hepatic Fibrosis via Inhibiting Hepatic Stellate Cells Proliferation and Activation.
      Title: CD73 Blockade Alleviated Hepatic Fibrosis via Inhibiting Hepatic Stellate Cells Proliferation and Activation.
    Submit: New GeneRIF Correction See all GeneRIFs (153)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (8) 
    • Targeted (6)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 5'-deoxynucleotidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 5'-deoxynucleotidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables 5'-nucleotidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5'-nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-nucleotidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables 5'-nucleotidase activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables GMP 5'-nucleotidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GMP 5'-nucleotidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables IMP 5'-nucleotidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables IMP 5'-nucleotidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables XMP 5'-nucleosidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ferrous iron binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thymidylate 5'-phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thymidylate 5'-phosphatase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in ADP catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in AMP catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within AMP catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in AMP catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in AMP catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ATP metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within adenosine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in adenosine biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in adenosine metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in calcium ion homeostasis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in inhibition of non-skeletal tissue mineralization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in inhibition of non-skeletal tissue mineralization ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in leukocyte cell-cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in leukocyte cell-cell adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within negative regulation of inflammatory response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to ATP IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell surface ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in external side of plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in synaptic membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    5'-nucleotidase
    Names
    5'-deoxynucleotidase
    IMP-specific 5'-nucleotidase
    ecto-5'-nucleotidase
    thymidylate 5'-phosphatase
    NP_035981.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_011851.4 → NP_035981.1  5'-nucleotidase preproprotein

      See identical proteins and their annotated locations for NP_035981.1

      Status: REVIEWED

      Source sequence(s)
      AK029979, BB850214
      Consensus CDS
      CCDS23386.1
      UniProtKB/Swiss-Prot
      Q3U3S1, Q61503
      UniProtKB/TrEMBL
      Q0VEE0
      Related
      ENSMUSP00000034992.7, ENSMUST00000034992.8
      Conserved Domains (3) summary
      COG0737
      Location:21 → 534
      UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
      cd07409
      Location:31 → 312
      MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
      pfam02872
      Location:354 → 509
      5_nucleotid_C; 5'-nucleotidase, C-terminal domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      88209662..88254142
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q61503.2 GenPept UniProtKB/Swiss-Prot:Q61503

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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