Spag9 sperm associated antigen 9 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Spag9provided by RGD
Official Full Name: sperm associated antigen 9provided by RGD
Primary source: RGD:1311289
See related: EnsemblRapid:ENSRNOG00000002749 AllianceGenome:RGD:1311289
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable several functions, including JUN kinase binding activity; kinesin binding activity; and signaling adaptor activity. Involved in negative regulation of dendrite extension; negative regulation of neuron differentiation; and negative regulation of protein phosphorylation. Predicted to be located in centriolar satellite; cytosol; and lysosomal membrane. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in hepatocellular carcinoma and liver cirrhosis. Orthologous to human SPAG9 (sperm associated antigen 9). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 300.1), Muscle (RPKM 276.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q26

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (79440311..79574709)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (78943439..79077797)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (81693736..81827562)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results show that JLP (Spag9) negatively regulates NGF-induced neurite outgrowth by decreasing the level of phosphorylated SCG10.
Title: Regulation of neurite outgrowth by interactions between the scaffolding protein, JNK-associated leucine zipper protein, and neuronal growth-associated protein superior cervical ganglia clone 10.

Submit: New GeneRIF Correction

General gene information

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Markers

RH140651 (e-PCR)
- UniSTS:222720

AI104446 (e-PCR)
- UniSTS:250649

AA925269 (e-PCR)
- UniSTS:261749

D10Rat114 (e-PCR)
- UniSTS:118151

RH140024 (e-PCR)
- UniSTS:222106

AA925269 (e-PCR)
- UniSTS:243381

RH134783 (e-PCR)
- UniSTS:218061

BE100739 (e-PCR)
- UniSTS:249848

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables JUN kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables JUN kinase binding	ISO Inferred from Sequence Orthology more info
enables MAP-kinase scaffold activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP-kinase scaffold activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinesin binding	IBA Inferred from Biological aspect of Ancestor more info
enables kinesin binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor complex adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in lysosome localization	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of dendrite extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within retrograde transport, endosome to Golgi	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within striated muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in lysosomal membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: C-Jun-amino-terminal kinase-interacting protein 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001108290.2 → NP_001101760.2 C-Jun-amino-terminal kinase-interacting protein 4

Status: VALIDATED

Source sequence(s)

CB605937, CB748350, FM062671, FM110716, JAXUCZ010000010

UniProtKB/TrEMBL

A0A8I5Y1R0

Related

ENSRNOP00000077913.2, ENSRNOT00000117102.2

Conserved Domains (3) summary

pfam07926
Location:56 → 162

TPR_MLP1_2; TPR/MLP1/MLP2-like protein

pfam09744
Location:25 → 178

Jnk-SapK_ap_N; JNK_SAPK-associated protein-1

pfam16471
Location:392 → 447

JIP_LZII; JNK-interacting protein leucine zipper II

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

79440311..79574709

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063269405.1 → XP_063125475.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X1

UniProtKB/TrEMBL

A0A0G2K6E8

Related

ENSRNOP00000073784.1, ENSRNOT00000085681.3
XM_063269407.1 → XP_063125477.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X3

UniProtKB/TrEMBL

A0A0G2K6E8
XM_063269406.1 → XP_063125476.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X2

UniProtKB/TrEMBL

A0A0G2K6E8, A0A8I5ZJK4

Related

ENSRNOP00000077739.1, ENSRNOT00000094632.2
XM_006247146.4 → XP_006247208.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X5

UniProtKB/TrEMBL

A0A0G2K6E8, A0A8I6B3N0

Related

ENSRNOP00000096035.1, ENSRNOT00000106993.2

Conserved Domains (3) summary

pfam07926
Location:56 → 162

TPR_MLP1_2; TPR/MLP1/MLP2-like protein

pfam09744
Location:25 → 178

Jnk-SapK_ap_N; JNK_SAPK-associated protein-1

pfam16471
Location:406 → 461

JIP_LZII; JNK-interacting protein leucine zipper II
XM_063269408.1 → XP_063125478.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X4

UniProtKB/TrEMBL

A0A8I5Y1R0, E9PSJ4

Related

ENSRNOP00000003777.6, ENSRNOT00000003777.8
XM_039086384.2 → XP_038942312.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X6

UniProtKB/TrEMBL

A0A8I5Y1R0

Conserved Domains (5) summary

COG0419
Location:33 → 520

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

pfam17380
Location:439 → 632

DUF5401; Family of unknown function (DUF5401)

pfam09744
Location:24 → 178

Jnk-SapK_ap_N; JNK_SAPK-associated protein-1

pfam16471
Location:396 → 451

JIP_LZII; JNK-interacting protein leucine zipper II

pfam19056
Location:942 → 1162

WD40_2; WD40 repeated domain
XM_006247147.4 → XP_006247209.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X7

UniProtKB/TrEMBL

A0A8I5Y1R0

Conserved Domains (3) summary

pfam07926
Location:56 → 162

TPR_MLP1_2; TPR/MLP1/MLP2-like protein

pfam09744
Location:25 → 178

Jnk-SapK_ap_N; JNK_SAPK-associated protein-1

pfam16471
Location:392 → 447

JIP_LZII; JNK-interacting protein leucine zipper II
XM_063269412.1 → XP_063125482.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X12

UniProtKB/TrEMBL

A0A8I6A5S1
XM_006247151.5 → XP_006247213.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X11

UniProtKB/TrEMBL

A0A8I6B484

Conserved Domains (1) summary

pfam16471
Location:249 → 304

JIP_LZII; JNK-interacting protein leucine zipper II
XM_006247154.5 → XP_006247216.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X14

UniProtKB/TrEMBL

A0A8I6B484

Related

ENSRNOP00000096462.1, ENSRNOT00000113361.2

Conserved Domains (1) summary

pfam16471
Location:249 → 304

JIP_LZII; JNK-interacting protein leucine zipper II
XM_063269410.1 → XP_063125480.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X9

UniProtKB/TrEMBL

A0A8I6A5S1

Related

ENSRNOP00000086317.2, ENSRNOT00000095843.2
XM_006247150.5 → XP_006247212.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X10

UniProtKB/TrEMBL

A0A8I6B484

Conserved Domains (1) summary

pfam16471
Location:253 → 308

JIP_LZII; JNK-interacting protein leucine zipper II
XM_006247153.5 → XP_006247215.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X13

UniProtKB/TrEMBL

A0A8I6B484

Conserved Domains (1) summary

pfam16471
Location:253 → 308

JIP_LZII; JNK-interacting protein leucine zipper II
XM_063269409.1 → XP_063125479.1 C-Jun-amino-terminal kinase-interacting protein 4 isoform X8

UniProtKB/TrEMBL

A0A8I6A5S1