Prkg1 protein kinase cGMP-dependent 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Prkg1provided by RGD
Full Name: protein kinase cGMP-dependent 1provided by RGD
Primary source: RGD:1587390
See related: EnsemblRapid:ENSRNOG00000053728 AllianceGenome:RGD:1587390
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pkgi; cGk1
Summary: Enables identical protein binding activity and mitogen-activated protein kinase p38 binding activity. Involved in several processes, including cell growth involved in cardiac muscle cell development; negative regulation of inositol phosphate biosynthetic process; and positive regulation of large conductance calcium-activated potassium channel activity. Located in acrosomal vesicle; nucleoplasm; and sarcolemma. Biomarker of allergic rhinitis; hypertension; hypogonadism; impotence; and obesity. Human ortholog(s) of this gene implicated in thoracic aortic aneurysm. Orthologous to human PRKG1 (protein kinase cGMP-dependent 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 103.4), Heart (RPKM 98.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q52

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (237818950..239052184, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (228409605..229638794, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (248982039..250300952, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Silencing of PKG1 Gene Mimics Effect of Aging and Sensitizes Rat Vascular Smooth Muscle Cells to Cardiotonic Steroids: Impact on Fibrosis and Salt Sensitivity.
Title: Silencing of PKG1 Gene Mimics Effect of Aging and Sensitizes Rat Vascular Smooth Muscle Cells to Cardiotonic Steroids: Impact on Fibrosis and Salt Sensitivity.
Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases.
Title: Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases.
melatonin ameliorated diabetic myocardial ischemia-reperfusion injury through cGMP-PKGIalpha, by modulating Nrf-2-HO-1 and MAPK signaling, thus reducing myocardial apoptosis and oxidative stress and preserving cardiac function
Title: Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation.
Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the iNOS and PKG-1 signaling pathway.
Title: Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide synthase and cyclic guanosine monophosphate-dependent protein kinase type 1.
These data suggest the involvement of calcium and PKGIalpha signaling in insulin-enhanced filtration barrier permeability in podocytes.
Title: Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGIα-dependent pathway.
During cGMP-mediated signaling, aa 888-928 of MYPT1 mediate the PKGIalpha-MYPT1 interaction.
Title: MYPT1 mutants demonstrate the importance of aa 888-928 for the interaction with PKGIalpha.
Our results demonstrate PKG-Ialpha might play an important role in the protective effects of scutellarin on endothelial dysfunction against myocardial ischemic reperfusion injury.
Title: Scutellarin's Cardiovascular Endothelium Protective Mechanism: Important Role of PKG-Iα.
The Golgi apparatus regulates PKGIalpha compartmentation and proteolysis.
Title: The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis.
Tumor cell implantation into the tibia induced upregulation of cGKI messenger ribonucleic acid and protein in dorsal root ganglia and hyperexcitability in DRG neurons. Continuing activation of this pathway is needed for hyperalgesia and/or allodynia.
Title: cGMP and cGMP-dependent protein kinase I pathway in dorsal root ganglia contributes to bone cancer pain in rats.
these data indicate that heparin effects on vascular smooth muscle cell proliferation depend, at least in part, on signaling through protein kinase G.
Title: Heparin responses in vascular smooth muscle cells involve cGMP-dependent protein kinase (PKG).

Submit: New GeneRIF Correction See all GeneRIFs (42)

General gene information

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Markers

BF404353 (e-PCR)
- UniSTS:247055

BF392245 (e-PCR)
- UniSTS:243495

D1Got245 (e-PCR)
- UniSTS:113725

RH133105 (e-PCR)
- UniSTS:216388

D1Uia16 (e-PCR)
- UniSTS:239948

D1Rat75 (e-PCR), detects polymorphism
- UniSTS:118610

Prkg1 (e-PCR), detects polymorphism
- UniSTS:525463

d1rat301 (e-PCR)
- UniSTS:121498

AU048249 (e-PCR)
- UniSTS:109723

d1rat371 (e-PCR)
- UniSTS:121515

D1Mgh25 (e-PCR)
- UniSTS:239282

D19Dcr84 (e-PCR), detects polymorphism
- UniSTS:546487

RH138063 (e-PCR)
- UniSTS:220150

BF396337 (e-PCR)
- UniSTS:245751

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	ISO Inferred from Sequence Orthology more info
enables cGMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables calcium channel regulator activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables mitogen-activated protein kinase p38 binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cell growth involved in cardiac muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cerebellum development	IEP Inferred from Expression Pattern more info	PubMed
involved_in collateral sprouting	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within forebrain development	ISO Inferred from Sequence Orthology more info
involved_in heart development	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of glutamate secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of inositol phosphate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of platelet aggregation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular associated smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular associated smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of large conductance calcium-activated potassium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of testosterone biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within relaxation of vascular associated smooth muscle	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
involved_in spermatid development	IEP Inferred from Expression Pattern more info	PubMed
involved_in vasodilation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cGMP-dependent protein kinase 1

Names: cAMP-dependent protein kinase catalytic subunit PRKX-like; cGMP dependent protein kinase type 1; protein kinase, cGMP-dependent, type 1; protein kinase, cGMP-dependent, type I

NP_001099201.3

EC 2.7.11.1

EC 2.7.11.12

XP_063128022.1

EC 2.7.11.1

EC 2.7.11.12

XP_063128025.1

EC 2.7.11.1

EC 2.7.11.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105731.4 → NP_001099201.3 cGMP-dependent protein kinase 1

See identical proteins and their annotated locations for NP_001099201.3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A0A8I6ATM1, A9LNM8

Related

ENSRNOP00000111855.1, ENSRNOT00000166788.1

Conserved Domains (4) summary

cd12085
Location:2 → 49

DD_cGKI-alpha; Dimerization/Docking domain of Cyclic GMP-dependent Protein Kinase I alpha

smart00133
Location:621 → 664

S_TK_X; Extension to Ser/Thr-type protein kinases

cd00038
Location:222 → 326

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:366 → 626

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)