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    RNY1 ribonuclease T2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855980, updated on 9-Dec-2024

    Summary

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    Official Symbol
    RNY1
    Official Full Name
    ribonuclease T2
    Primary source
    SGD:S000006044
    Locus tag
    YPL123C
    See related
    AllianceGenome:SGD:S000006044; FungiDB:YPL123C; VEuPathDB:YPL123C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables RNA endonuclease activity. Involved in RNA catabolic process; apoptotic process; and cell morphogenesis. Acts upstream of or within mRNA catabolic process. Located in cytosol; extracellular region; and fungal-type vacuole. Human ortholog(s) of this gene implicated in several diseases, including Lynch syndrome; RNASET2-deficient cystic leukoencephalopathy; colorectal cancer (multiple); lung cancer; and mismatch repair cancer syndrome. Orthologous to human RNASET2 (ribonuclease T2). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See RNY1 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    1
    Sequence:
    Chromosome: XVI; NC_001148.4 (317641..318945, complement)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene karyopherin KAP120 Neighboring gene Spc29p Neighboring gene TFIIH/NER complex subunit TFB2 Neighboring gene Mei5p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure-function analysis of Rny1 in tRNA cleavage and growth inhibition. Luhtala N, et al. PLoS One, 2012. PMID 22829915, Free PMC Article
    2. Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: unexpected functions for ancient enzymes? MacIntosh GC, et al. Proc Natl Acad Sci U S A, 2001 Jan 30. PMID 11158587, Free PMC Article
    3. Golgi-mediated glycosylation determines residency of the T2 RNase Rny1p in Saccharomyces cerevisiae. Shcherbik N. Traffic, 2013 Dec. PMID 24102742
    4. Selectivity of mRNA degradation by autophagy in yeast. Makino S, et al. Nat Commun, 2021 Apr 19. PMID 33875662, Free PMC Article
    5. Widespread Co-translational RNA Decay Reveals Ribosome Dynamics. Pelechano V, et al. Cell, 2015 Jun 4. PMID 26046441, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Glycosylation of N7, N70, N103 and N123 is critical for Rny1 extracellular secretion.
      Title: Golgi-mediated glycosylation determines residency of the T2 RNase Rny1p in Saccharomyces cerevisiae.
    2. Biochemical flotation assays reveal that in rny1Delta cells, some tRNA molecules associate with membranes suggesting that cleavage of tRNAs by Rny1 can involve either tRNA association with, or uptake into, membrane compartments.
      Title: Structure-function analysis of Rny1 in tRNA cleavage and growth inhibition.
    3. Rny1p is released from the vacuole into the cytosol and cleaves tRNAs during oxidative stress.
      Title: The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA endonuclease activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribonuclease T2 activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in apoptotic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in cell morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within mRNA catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in extracellular region IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in extracellular region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in vacuolar lumen IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ribonuclease T2
    NP_015202.1
    • Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; required for tRNA-specific translational pausing during oxidative stress; promotes apoptosis under stress conditions and this function is independent of Rny1p catalytic activity

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      317641..318945 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183937.1 → NP_015202.1  TPA: ribonuclease T2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015202.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W3P4, Q02933
      UniProtKB/TrEMBL
      B3LKV1, B5VT42, C7GJ77, C8ZIQ6, N1NVG5
      Conserved Domains (1) summary
      cd01061
      Location:50 → 284
      RNase_T2_euk; Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q02933.1

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