SRV2 adenylate cyclase-binding protein [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SRV2
Official Full Name: adenylate cyclase-binding protein
Primary source: SGD:S000005082
Locus tag: YNL138W
See related: AllianceGenome:SGD:S000005082; FungiDB:YNL138W; VEuPathDB:YNL138W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CAP
Summary: Enables actin binding activity and adenylate cyclase binding activity. Involved in several processes, including Ras protein signal transduction; positive regulation of mitochondrial fission; and regulation of actin polymerization or depolymerization. Located in actin cortical patch. Used to study ovary epithelial cancer. Human ortholog(s) of this gene implicated in dilated cardiomyopathy. Orthologous to human CAP1 (cyclase associated actin cytoskeleton regulatory protein 1) and CAP2 (cyclase associated actin cytoskeleton regulatory protein 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SRV2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (366741..368321)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cyclase-associated protein (CAP) and Cofilin synergize to processively depolymerize actin filament pointed ends at a rate 330-fold faster than spontaneous depolymerization. Single molecule imaging further reveals that hexameric CAP molecules interact with the pointed ends of Cofilin-decorated filaments for several seconds at a time, removing approximately 100 actin subunits per binding event.
Title: Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude.
in vivo roles of Srv2p in the regulation of the actin cytoskeleton during clathrin-mediated endocytosis.
Title: Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis.
Data show that Twinfilin and Srv2 proteins accelerate depolymerization from actin filament ends.
Title: High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP.
Data indicate that the combined activities of cofilin and Srv2/CAP lead to enhanced filament severing and support an emerging view that actin disassembly is controlled not by cofilin alone, but by a more complex set of factors working in concert.
Title: Srv2/cyclase-associated protein forms hexameric shurikens that directly catalyze actin filament severing by cofilin.
Data suggest a revised model for the mechanism by which Srv2 promotes actin turnover, in which coordinated activities of its N- and C-terminal halves catalyze sequential steps in recycling cofilin and actin monomers.
Title: Reconstitution and dissection of the 600-kDa Srv2/CAP complex: roles for oligomerization and cofilin-actin binding in driving actin turnover.
Srv2/cyclase-associated protein has a high-affinity interaction with ADP-actin monomers
Title: A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein.
The mechanism and biological interaction of profilin and SRV2 protein were studied.
Title: Mechanism and biological role of profilin-Srv2/CAP interaction.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables adenylate cyclase binding	IBA Inferred from Biological aspect of Ancestor more info
enables adenylate cyclase binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament severing	IDA Inferred from Direct Assay more info	PubMed
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed

General protein information

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Preferred Names: adenylate cyclase-binding protein

NP_014261.1

Cyclase-associated protein, CAP; N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; N- and C-termini can function as physically separate proteins; mCAP1 is the mouse homolog

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

366741..368321

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182976.1 → NP_014261.1 TPA: adenylate cyclase-binding protein [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014261.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W144, P17555

UniProtKB/TrEMBL

B3LNZ5, B5VQR1, C7GKA9, C8ZG92, N1NWI0

Conserved Domains (2) summary

pfam01213
Location:14 → 96

CAP_N; Adenylate cyclase associated (CAP) N terminal

pfam08603
Location:369 → 524

CAP_C; Adenylate cyclase associated (CAP) C terminal