PolE1 DNA polymerase epsilon subunit 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: PolE1provided by FlyBase
Official Full Name: DNA polymerase epsilon subunit 1provided by FlyBase
Primary source: FLYBASE:FBgn0264326
Locus tag: Dmel_CG6768
See related: AllianceGenome:FB:FBgn0264326
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 6768; BAB17608; CG6768; Dmel\CG6768; DNA pol epsilon; DNApol-epsilon; DNApol-epsilon255; dpolepsilon; dpolepsilon255; dpolepsilonp255; l(3)pl10; l(3)pl10R; pl[10]; pol-epsilon; Polepsilon; Polepsilon255; Polepsilon255KDa
Summary: Enables 3'-5'-DNA exonuclease activity and DNA-directed DNA polymerase activity. Involved in DNA replication proofreading; DNA synthesis involved in DNA replication; and endomitotic cell cycle. Located in nucleus. Part of epsilon DNA polymerase complex. Is expressed in embryonic/larval Malpighian tubule; embryonic/larval salivary gland; gamete; imaginal disc; and larval brain. Human ortholog(s) of this gene implicated in colorectal cancer (multiple); endometrial cancer (multiple); lung cancer (multiple); and mismatch repair cancer syndrome. Orthologous to human POLE (DNA polymerase epsilon, catalytic subunit). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 94E13-94E13; 3-78 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (23348128..23355163)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (19173850..19180885)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N-terminal catalytic domain of the DNA polymerase epsilon p255 subunit plays role in the G1-S transition and/or S-phase progression of the mitotic cycle.
Title: Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 3'-5'-DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA 3'-5' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication proofreading	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication proofreading	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA synthesis involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair, gap-filling	IBA Inferred from Biological aspect of Ancestor more info
involved_in endomitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leading strand elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair, DNA gap filling	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of epsilon DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of epsilon DNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of epsilon DNA polymerase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA polymerase epsilon subunit 1

Names: CG6768-PA; DNA polymerase epsilon; DNA polymerase epsilon 255kD subunit; DNApol-epsilon; DNApolymarase E; PolE1-PA; lethal (3) pl10; pol.epsilon catalytic subunit

NP_524462.2

EC 2.7.7.7

EC 3.1.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

23348128..23355163

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079738.3 → NP_524462.2 DNA polymerase epsilon subunit 1 [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524462.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95R46, Q9VCN1

UniProtKB/TrEMBL

G3M399

Related

FBpp0083800

Conserved Domains (3) summary

cd05779
Location:266 → 469

DNA_polB_epsilon_exo; DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon, a family-B DNA polymerase

cd05535
Location:529 → 1154

POLBc_epsilon; DNA polymerase type-B epsilon subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) epsilon has been proposed ...

pfam08490
Location:1529 → 1930

DUF1744; Domain of unknown function (DUF1744)