Appl2 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Appl2provided by MGI
Official Full Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2provided by MGI
Primary source: MGI:MGI:2384914
See related: Ensembl:ENSMUSG00000020263 AllianceGenome:MGI:2384914
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dip3b; Dip3 beta; DIP13 beta
Summary: Predicted to enable phospholipid binding activity; protein homodimerization activity; and small GTPase binding activity. Involved in several processes, including adiponectin-activated signaling pathway; negative regulation of metabolic process; and positive regulation of endocytosis. Located in early phagosome membrane; macropinosome; and ruffle membrane. Is expressed in several structures, including alimentary system; autopod; brain; neural retina; and ventricular layer. Orthologous to human APPL2 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in cerebellum adult (RPKM 26.5), adrenal adult (RPKM 22.2) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 41.29 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (83435897..83484741, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (83600033..83648877, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Adiponectin Alleviates Diet-Induced Inflammation in the Liver by Suppressing MCP-1 Expression and Macrophage Infiltration.
Title: Adiponectin Alleviates Diet-Induced Inflammation in the Liver by Suppressing MCP-1 Expression and Macrophage Infiltration.
APPL2 Negatively Regulates Olfactory Functions by Switching Fate Commitments of Neural Stem Cells in Adult Olfactory Bulb via Interaction with Notch1 Signaling.
Title: APPL2 Negatively Regulates Olfactory Functions by Switching Fate Commitments of Neural Stem Cells in Adult Olfactory Bulb via Interaction with Notch1 Signaling.
The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic beta-cells.
Title: The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic β-cells.
APPL2 overexpression could blunt the activation of glucocorticoid receptor when undergoing environmental stress.
Title: Adaptor Protein APPL2 Affects Adult Antidepressant Behaviors and Hippocampal Neurogenesis via Regulating the Sensitivity of Glucocorticoid Receptor.
Here we establish potential roles for APPL1 and 2 signaling adaptors as regulators of LPS/TLR4-induced signaling, transcription, and cytokine secretion.
Title: Distinct Roles for APPL1 and APPL2 in Regulating Toll-like Receptor 4 Signaling in Macrophages.
Appl1/2-null mouse embryonic fibroblasts exhibited defects in HGF-induced Akt activation, migration, and invasion.
Title: Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts.
Rab31, with APPL2 as its effector, play a role in FcgammaR-mediated phagocytosis through PI3K/Akt signaling in macrophages.
Title: Rab31 and APPL2 enhance FcγR-mediated phagocytosis through PI3K/Akt signaling in macrophages.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol binding	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidylserine binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylserine binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adiponectin-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hepatocyte growth factor stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cold acclimation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in diet induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of D-glucose import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of D-glucose import	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of D-glucose import	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cellular response to insulin stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cytokine production involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of fatty acid oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neural precursor cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macropinocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phagocytosis, engulfment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein homotetramerization	ISO Inferred from Sequence Orthology more info
involved_in protein homotetramerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein import into nucleus	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of G1/S transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of G1/S transition of mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of fibroblast migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of toll-like receptor 4 signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in transforming growth factor beta receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISS Inferred from Sequence or Structural Similarity more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in early phagosome	IDA Inferred from Direct Assay more info	PubMed
located_in early phagosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
is_active_in endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome membrane	ISO Inferred from Sequence Orthology more info
located_in endosome membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in macropinosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle membrane	IDA Inferred from Direct Assay more info	PubMed
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DCC-interacting protein 13-beta

Names: adapter protein containing PH domain, PTB domain and leucine zipper motif 2; dip13-beta

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145220.2 → NP_660255.1 DCC-interacting protein 13-beta

See identical proteins and their annotated locations for NP_660255.1

Status: VALIDATED

Source sequence(s)

AY113706

Consensus CDS

CCDS24078.1

UniProtKB/Swiss-Prot

Q8K3G9, Q99LT7

UniProtKB/TrEMBL

Q3TB15, Q3TGG0, Q3TVI6

Related

ENSMUSP00000020500.8, ENSMUST00000020500.14

Conserved Domains (4) summary

cd07632
Location:20 → 234

BAR_APPL2; The Bin/Amphiphysin/Rvs (BAR) domain of Adaptor protein, Phosphotyrosine interaction, PH domain and Leucine zipper containing 2

cd13158
Location:480 → 613

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:252 → 376

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

pfam00169
Location:278 → 378

PH; PH domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

83435897..83484741 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513511.5 → XP_006513574.1 DCC-interacting protein 13-beta isoform X5

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:370 → 503

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:142 → 266

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 124

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155748.1 → XP_036011641.1 DCC-interacting protein 13-beta isoform X3

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:427 → 560

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:199 → 323

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 181

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_006513508.5 → XP_006513571.1 DCC-interacting protein 13-beta isoform X1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:455 → 588

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:227 → 351

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:27 → 209

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155746.1 → XP_036011639.1 DCC-interacting protein 13-beta isoform X1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:455 → 588

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:227 → 351

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:27 → 209

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155747.1 → XP_036011640.1 DCC-interacting protein 13-beta isoform X1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:455 → 588

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:227 → 351

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:27 → 209

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_030245027.2 → XP_030100887.1 DCC-interacting protein 13-beta isoform X3

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:427 → 560

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:199 → 323

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 181

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155745.1 → XP_036011638.1 DCC-interacting protein 13-beta isoform X1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:455 → 588

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:227 → 351

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:27 → 209

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_006513510.4 → XP_006513573.1 DCC-interacting protein 13-beta isoform X3

See identical proteins and their annotated locations for XP_006513573.1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:427 → 560

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:199 → 323

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 181

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_030245026.2 → XP_030100886.1 DCC-interacting protein 13-beta isoform X3

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:427 → 560

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:199 → 323

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 181

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155744.1 → XP_036011637.1 DCC-interacting protein 13-beta isoform X1

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:455 → 588

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:227 → 351

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:27 → 209

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_036155749.1 → XP_036011642.1 DCC-interacting protein 13-beta isoform X5

UniProtKB/TrEMBL

Q3TB15, Q3TGG0

Conserved Domains (3) summary

cd13158
Location:370 → 503

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:142 → 266

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 124

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_006513509.4 → XP_006513572.1 DCC-interacting protein 13-beta isoform X2

UniProtKB/TrEMBL

Q3TB15

Conserved Domains (3) summary

cd07632
Location:20 → 234

BAR_APPL2; The Bin/Amphiphysin/Rvs (BAR) domain of Adaptor protein, Phosphotyrosine interaction, PH domain and Leucine zipper containing 2

cd13158
Location:480 → 613

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:252 → 376

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain
XM_017313895.3 → XP_017169384.1 DCC-interacting protein 13-beta isoform X4

UniProtKB/TrEMBL

Q3TB15

Conserved Domains (3) summary

cd13158
Location:427 → 560

PTB_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif (APPL; also called DCC-interacting protein (DIP)-13alpha) Phosphotyrosine-binding (PTB) domain

cd13247
Location:199 → 323

BAR-PH_APPL; Adaptor protein containing PH domain, PTB domain, and Leucine zipper motif Bin1/amphiphysin/Rvs167 (BAR)-Pleckstrin homology (PH) domain

cl12013
Location:1 → 181

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature