NDC80 kinetochore-associated Ndc80 complex subunit NDC80 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NDC80
Official Full Name: kinetochore-associated Ndc80 complex subunit NDC80
Primary source: SGD:S000001406
Locus tag: YIL144W
See related: AllianceGenome:SGD:S000001406; FungiDB:YIL144W; VEuPathDB:YIL144W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HEC1; TID3
Summary: Contributes to microtubule binding activity. Involved in chromosome segregation and protein localization to kinetochore. Located in kinetochore. Part of Ndc80 complex. Used to study cancer. Orthologous to human NDC80 (NDC80 kinetochore complex component). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NDC80 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (78074..80149)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Title: Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Title: Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.
Title: Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.
Meiotic regulation of the Ndc80 complex composition and function.
Title: Meiotic regulation of the Ndc80 complex composition and function.
Our results suggest the stoichiometry of outer kinetochore submodules is strongly influenced by factors at the kinetochore-microtubule interface such as Fin1 and Dam1, and phosphorylation by cyclin-dependent kinase.
Title: The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.
The authors suggest that prior to its assembly at the kinetochore, the Ndc80 complex interchanges between bent (auto-inhibited) and open conformations. Once assembled, its association with MIND stabilizes the Ndc80 complex in a straightened form for higher affinity microtubule binding.
Title: Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules.
We used this assay to investigate why kinetochores recruit the microtubule-binding Ndc80 complex via two receptors: the Mis12 complex and CENP-T. Although the CENP-T pathway is non-essential in yeast, we demonstrate that it becomes essential for viability and Ndc80c recruitment when the Mis12 pathway is crippled by defects in Dsn1 phosphorylation
Title: An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.
The authors discovered that in budding yeast, kinetochore inactivation during meiosis occurs by reducing the abundance of a limiting subunit, Ndc80.
Title: Kinetochore inactivation by expression of a repressive mRNA.
Thus, expression of a 5' extended mRNA isoform causes transcriptional interference at the downstream promoter of NDC80. The authors demonstrate that this is an effective mechanism to promote dynamic changes in gene expression during cell differentiation.
Title: Transcription of a 5' extended mRNA isoform directs dynamic chromatin changes and interference of a downstream promoter.
The authors show that the Ndc80 complex can simultaneously bind and bridge across two Dam1 complex rings through a tripartite interaction, each component of which is regulated by Aurora B kinase.
Title: The Ndc80 complex bridges two Dam1 complex rings.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to microtubule binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of mitotic spindle microtubules to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
involved_in attachment of mitotic spindle microtubules to kinetochore	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Ndc80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ndc80 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Ndc80 complex	IEA Inferred from Electronic Annotation more info
part_of Ndc80 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: kinetochore-associated Ndc80 complex subunit NDC80

NP_012122.3

Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

78074..80149

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179492.3 → NP_012122.3 TPA: kinetochore-associated Ndc80 complex subunit NDC80 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012122.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVE3, P40460

UniProtKB/TrEMBL

A6ZVD2

Conserved Domains (1) summary

COG5185
Location:1 → 683

HEC1; Protein involved in chromosome segregation, interacts with SMC proteins [Cell cycle control, cell division, chromosome partitioning]