CHS3 chitin synthase CHS3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CHS3
Official Full Name: chitin synthase CHS3
Primary source: SGD:S000000227
Locus tag: YBR023C
See related: AllianceGenome:SGD:S000000227; FungiDB:YBR023C; VEuPathDB:YBR023C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CAL1; CSD2; DIT101; KTI2
Summary: Enables chitin synthase activity. Involved in ascospore wall assembly; chitin biosynthetic process; and protein localization to bud neck. Located in several cellular components, including chitosome; incipient cellular bud site; and prospore membrane. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CHS3 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (284428..287925, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We analyzed the dynamic localization and interaction of Chs3 and Chs4 during cell division, and found that both proteins transiently co-localize and physically interact only during bud formation and later in a period during septum formation and cytokinesis
Title: Septin-associated protein kinase Gin4 affects localization and phosphorylation of Chs4, the regulatory subunit of the Baker's yeast chitin synthase III complex.
that Chs7 engages in functionally distinct interactions with Chs3 to first promote its folding and endoplasmic reticulum exit, and subsequently to regulate its activity at the plasma membrane
Title: The chaperone Chs7 forms a stable complex with Chs3 and promotes its activity at the cell surface.
Hof1 binds to the Sel1-like repeats (SLRs) of Chs4 via its F-BAR domain and inhibits Chs3-mediated chitin synthesis during cytokinesis. In addition, Hof1 is required for rapid accumulation as well as efficient removal of Chs4 at the division site.
Title: Hof1 and Chs4 Interact via F-BAR Domain and Sel1-like Repeats to Control Extracellular Matrix Deposition during Cytokinesis.
Synthesis of chitin by Chs3, the chitin synthase responsible for chitosan layer formation, is dependent on the cis-prenyltransferase activity of Srt1, indicating that polyprenols are necessary to coordinate assembly of the spore wall layers.
Title: Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.
the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.
Title: Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.
data show that the oligomerization of Chs3 through its N-terminus is essential for proper protein trafficking and chitin synthesis and is therefore monitored intracellularly.
Title: Oligomerization of the chitin synthase Chs3 is monitored at the Golgi and affects its endocytic recycling.
The anchoring of Chs3 to septins through Chs4 prevents the arrival of this protein at endocytic sites.
Title: Neck compartmentalization as the molecular basis for the different endocytic behaviour of Chs3 during budding or hyperpolarized growth in yeast cells.
the essential role of Bni4 is to target Glc7 to the neck and activate it toward substrates necessary for CSIII recruitment and synthesis of chitin at the bud neck
Title: Protein phosphatase type 1 directs chitin synthesis at the bud neck in Saccharomyces cerevisiae.
Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Title: Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Results suggest that Chs4p promotes Chs3p translocation into the plasma membrane in a stable and active form, and that proper chitin synthase III turnover is maintained through the endocytic internalization of Chs3p.
Title: Chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chitin synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables chitin synthase activity	IEA Inferred from Electronic Annotation more info
enables chitin synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ascospore wall assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in chitin biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in chitin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to bud neck	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell periphery	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cell septum	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in chitosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in prospore membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: chitin synthase CHS3

NP_009579.1

EC 2.4.1.16

Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

284428..287925 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178371.1 → NP_009579.1 TPA: chitin synthase CHS3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009579.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ25, P29465

UniProtKB/TrEMBL

A6ZKW9, C7GM29, C8Z417

Conserved Domains (1) summary

pfam03142
Location:628 → 1151

Chitin_synth_2; Chitin synthase