ADA2 chromatin-binding transcription regulator ADA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ADA2
Official Full Name: chromatin-binding transcription regulator ADA2
Primary source: SGD:S000002856
Locus tag: YDR448W
See related: AllianceGenome:SGD:S000002856; FungiDB:YDR448W; VEuPathDB:YDR448W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SWI8
Summary: Enables chromatin binding activity; phosphatidylserine binding activity; and transcription coactivator activity. Involved in heterochromatin formation; regulation of transcription by RNA polymerase II; and replication-born double-strand break repair via sister chromatid exchange. Part of ADA complex; SAGA complex; and SLIK (SAGA-like) complex. Orthologous to several human genes including TADA2B (transcriptional adaptor 2B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ADA2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1356065..1357369)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the Ada2 SANT domain does not activate Gcn5's activity by directly affecting histone peptide binding as previously proposed. Instead, the Ada2 SANT domain enhances Gcn5 binding of the enzymatic cosubstrate acetyl-CoA.
Title: Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA histone acetyltransferase complexes. SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins.
Title: Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA.
Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
Title: Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
Ada2, likely in the context of SAGA, is positioned at chromosomal termini to participate in both transcriptional repression and activation in response to nutrient signaling.
Title: The SAGA subunit Ada2 functions in transcriptional silencing.
Indicative of Ada2 affecting Gcn5 function, Ada2 mutation resulted in a decrease in Gcn5-mediated histone acetylation in vitro to a level approximately 40% that with wild-type Ada2.
Title: A conserved central region of yeast Ada2 regulates the histone acetyltransferase activity of Gcn5 and interacts with phospholipids.
Ada2p regulates transcription and DNA repair at the MET16 gene.
Title: Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylserine binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylserine binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coactivator activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in rDNA heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication-born double-strand break repair via sister chromatid exchange	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of ADA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA-type complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SLIK (SAGA-like) complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: chromatin-binding transcription regulator ADA2

NP_010736.3

Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1356065..1357369

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180756.3 → NP_010736.3 TPA: chromatin-binding transcription regulator ADA2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010736.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT73, Q02336

UniProtKB/TrEMBL

A6ZZ39, B3LFL4, B5VGX9, C7GKP0, C8Z606, G2WBH9, N1PAK3

Conserved Domains (1) summary

COG5114
Location:3 → 434

COG5114; Histone acetyltransferase complex SAGA/ADA, subunit ADA2 [Chromatin structure and dynamics]