PPN1 endopolyphosphatase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PPN1
Official Full Name: endopolyphosphatase
Primary source: SGD:S000002860
Locus tag: YDR452W
See related: AllianceGenome:SGD:S000002860; FungiDB:YDR452W; VEuPathDB:YDR452W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PHM5
Summary: Enables endopolyphosphatase activity and exopolyphosphatase activity. Involved in polyphosphate catabolic process. Located in cytosol; fungal-type vacuole membrane; and nucleus. Human ortholog(s) of this gene implicated in Niemann-Pick disease; Niemann-Pick disease type A; and Niemann-Pick disease type B. Orthologous to several human genes including SMPDL3A (sphingomyelin phosphodiesterase acid like 3A) and SMPDL3B (sphingomyelin phosphodiesterase acid like 3B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PPN1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1362878..1364902)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

endopolyphosphatase cleaved inorganic polyphosphate without the release of orthophosphate and was inhibited by heparin
Title: Properties of partially purified endopolyphosphatase of the yeast Saccharomyces cerevisiae.
PPX1 and PPN1 genes encoding exopolyphosphatases.
Title: Finding of endopolyphosphatase activity in the yeast Saccharomyces cerevisiae.
Exopolyphosphatase incoded by ppx1 and ppn1 genes do not take part in polyphsphate biosynthesis.
Title: [Polyphosphate consumption and accumulation in Saccharomyces cerevisiae cytosol under inactivation of exopolyphosphatase genes PPX1 and PPN1].
S. cerevisiae possesses a system, which makes it possible to compensate for inactivation of the PPX1 and PPN1 genes encoding exopolyphosphatases of the yeast cells.
Title: Inactivation of PPX1 and PPN1 genes encoding exopolyphosphatases of Saccharomyces cerevisiae does not prevent utilization of polyphosphates as phosphate reserve.
Influence of carbon source on the activity of PPN1.
Title: Polyphosphates and exopolyphosphatases in cytosol and mitochondria of Saccharomyces cerevisiae during growth on glucose or ethanol under phosphate surplus.
In the single PPN1 mutant, a considerable decrease of exopolyphosphatase activity was observed in all the compartments under study.
Title: Inorganic polyphosphates and exopolyphosphatases in cell compartments of the yeast Saccharomyces cerevisiae under inactivation of PPX1 and PPN1 genes.
It has been shown that the high molecular mass exopolyphosphatase localized in cytosol of the yeast Saccharomyces cerevisiae is encoded by the PPN1 gene.
Title: High molecular mass exopolyphosphatase from the cytosol of the yeast Saccharomyces cerevisiae is encoded by the PPN1 gene.
Both termini of prePpn1 and the post-translational modification of N-glycosylations are essential for the protease-mediated maturation of Ppn1.
Title: Endopolyphosphatase in Saccharomyces cerevisiae undergoes post-translational activations to produce short-chain polyphosphates.
Inactivation of the PPX1 gene encoding exopolyphosphatase had no effect on respiration functions and on polyphosphate level and chain length in mitochondria.
Title: Effects of inactivation of the PPN1 gene on exopolyphosphatases, inorganic polyphosphates and function of mitochondria in the yeast Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables endopolyphosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables endopolyphosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables endopolyphosphatase activity	IEA Inferred from Electronic Annotation more info
enables endopolyphosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables exopolyphosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables exopolyphosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables exopolyphosphatase activity	IEA Inferred from Electronic Annotation more info
enables exopolyphosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in polyphosphate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in polyphosphate catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in polyphosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in polyphosphate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole	HDA more info	PubMed
is_active_in fungal-type vacuole	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: endopolyphosphatase

NP_010740.3

EC 3.6.1.10

EC 3.6.1.11

Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1362878..1364902

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180760.3 → NP_010740.3 TPA: endopolyphosphatase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010740.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT77, Q04119

UniProtKB/TrEMBL

A6ZZ43, B3LFL1, C7GKP4, C8Z610, N1PAK4

Conserved Domains (1) summary

cd00842
Location:97 → 375

MPP_ASMase; acid sphingomyelinase and related proteins, metallophosphatase domain