Pla2g10 phospholipase A2, group X [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pla2g10provided by MGI
Official Full Name: phospholipase A2, group Xprovided by MGI
Primary source: MGI:MGI:1347522
See related: Ensembl:ENSMUSG00000022683 AllianceGenome:MGI:1347522
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PLA2GX; sPLA2-X; GX sPLA2
Summary: This gene encodes a member of the phospholipase A2 family of lipolytic enzymes that hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. The encoded protein undergoes proteolytic processing to generate a calcium-dependent enzyme that plays pivotal roles in the liberation of arachidonic acid from membrane phospholipids leading to the production of various inflammatory lipid mediators, such as prostaglandins. In response to myocardial ischemia/reperfusion, mice lacking the encoded protein display a reduction in myocardial infarct size partly through the suppression of neutorphil cytotoxic activities. Alternative splicing results in multiple transcript variants encoding different isoforms. All of these isoforms may undergo similar processing to generate the mature protein. [provided by RefSeq, Jul 2015]
Expression: Biased expression in colon adult (RPKM 60.5), testis adult (RPKM 58.5) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 A1; 16 9.5 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (13532921..13548863, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (13715057..13731094, complement)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

secretory PLA2s have important functions as genetic modifiers of inflammation and colon cancer.
Title: Secreted Phospholipases A2 Are Intestinal Stem Cell Niche Factors with Distinct Roles in Homeostasis, Inflammation, and Cancer.
PLA2G10 releases omega-3 polyunsaturated fatty acids, suppresses colitis, and promotes sperm fertility.
Title: Group X Secreted Phospholipase A2 Releases ω3 Polyunsaturated Fatty Acids, Suppresses Colitis, and Promotes Sperm Fertility.
Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2beta (iPLA2beta) and Group X Secreted Phospholipase A2 (sPLA2).
Title: Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2β (iPLA2β) and Group X Secreted Phospholipase A2 (sPLA2).
GX sPLA2 negatively regulated pancreatic insulin secretion by augmenting COX-2-dependent PGE2 production.
Title: Group X secretory phospholipase A2 regulates insulin secretion through a cyclooxygenase-2-dependent mechanism.
Mouse PLA2GX has a unique property of improving fertilization outcome among other secreted phospholipase A2 isoforms.
Title: The effect of group X secreted phospholipase A2 on fertilization outcome is specific and not mimicked by other secreted phospholipases A2 or progesterone.
Data indicate that experimental allergic bronchitis (EAB) in was associated with increased expression of sPLA2, specifically sPLA2gX, in the lungs.
Title: Phospholipase A2 in experimental allergic bronchitis: a lesson from mouse and rat models.
Authors propose that activation of GX-sPLA2 during H1N1pdm infection is an early step of pulmonary inflammation and its inhibition increases adaptive immunity and improves survival.
Title: Lack of group X secreted phospholipase A₂ increases survival following pandemic H1N1 influenza infection.
Pla2G10 limits the development of atherosclerosis in LDL receptor-null mice.
Title: Group X secreted phospholipase A2 limits the development of atherosclerosis in LDL receptor-null mice.
the use of a highly potent indole-based inhibitor of hGX-sPLA(2), RO061606 (which is ineffective against mGX-sPLA(2)), to assess the potential utility of GX-sPLA(2) blockade as a therapeutic intervention in asthma.
Title: Blockade of human group X secreted phospholipase A2 (GX-sPLA2)-induced airway inflammation and hyperresponsiveness in a mouse asthma model by a selective GX-sPLA2 inhibitor.
sPLA(2)-X in neutrophils plays a pathogenic role in abdominal aortic aneurysms in a mouse model.
Title: Group X secretory PLA2 in neutrophils plays a pathogenic role in abdominal aortic aneurysms in mice.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (2) 1 citation
Targeted (4) 1 citation

General gene information

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Markers

Pla2g10 (e-PCR), detects polymorphism
- UniSTS:527493

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 1-alkyl-2-acetylglycerophosphocholine esterase activity	ISO Inferred from Sequence Orthology more info
enables 1-alkyl-2-acetylglycerophosphocholine esterase activity	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent phospholipase A2 activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent phospholipase A2 activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent phospholipase A2 activity	ISO Inferred from Sequence Orthology more info
enables calcium-independent phospholipase A2 activity	IEA Inferred from Electronic Annotation more info
enables phospholipase A2 activity	ISO Inferred from Sequence Orthology more info
enables phospholipase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in arachidonate secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within axon guidance	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cholesterol homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to virus	IEA Inferred from Electronic Annotation more info
involved_in defense response to virus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within erythrocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hair follicle morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intestinal stem cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in low-density lipoprotein particle remodeling	IEA Inferred from Electronic Annotation more info
involved_in low-density lipoprotein particle remodeling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lysophospholipid transport	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within macrophage activation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cholesterol efflux	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cytokine production involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylcholine catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylcholine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylcholine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylethanolamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylethanolamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylethanolamine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylserine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylserine metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phospholipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in platelet activating factor catabolic process	ISO Inferred from Sequence Orthology more info
involved_in platelet activating factor catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of acrosome reaction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of arachidonate secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of lipid storage	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of lipid storage	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of prostaglandin secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of prostaglandin secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within production of molecular mediator involved in inflammatory response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within production of molecular mediator involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in prostaglandin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of macrophage activation	IEA Inferred from Electronic Annotation more info
involved_in regulation of macrophage activation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in lysosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: group 10 secretory phospholipase A2

Names: group X secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 10

NP_001277938.1

EC 3.1.1.4

NP_036117.1

EC 3.1.1.4

XP_017172476.1

EC 3.1.1.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001291009.2 → NP_001277938.1 group 10 secretory phospholipase A2 isoform 2 preproprotein

See identical proteins and their annotated locations for NP_001277938.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC154607, AK076921, AV075661

Consensus CDS

CCDS70684.1

UniProtKB/TrEMBL

Q8K130

Related

ENSMUSP00000111474.3, ENSMUST00000115807.9

Conserved Domains (1) summary

cd00125
Location:29 → 111

PLA2c; PLA2c: Phospholipase A2, a family of secretory and cytosolic enzymes; the latter are either Ca dependent or Ca independent. PLA2 cleaves the sn-2 position of the glycerol backbone of phospholipids (PC or phosphatidylethanolamine), usually in a ...
NM_011987.4 → NP_036117.1 group 10 secretory phospholipase A2 isoform 1 preproprotein

See identical proteins and their annotated locations for NP_036117.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1).

Source sequence(s)

AC154607, AK076921, AV075661

Consensus CDS

CCDS27967.1

UniProtKB/Swiss-Prot

Q9EQK6, Q9QXX3

UniProtKB/TrEMBL

Q8C5Y6

Related

ENSMUSP00000023364.7, ENSMUST00000023364.7

Conserved Domains (1) summary

cd00125
Location:29 → 143

PLA2c; PLA2c: Phospholipase A2, a family of secretory and cytosolic enzymes; the latter are either Ca dependent or Ca independent. PLA2 cleaves the sn-2 position of the glycerol backbone of phospholipids (PC or phosphatidylethanolamine), usually in a ...

RNA

NR_110990.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC154607, AK076921, AV075661

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000082.7 Reference GRCm39 C57BL/6J

Range

13532921..13548863 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316987.3 → XP_017172476.1 group 10 secretory phospholipase A2 isoform X1

Conserved Domains (1) summary

cd00125
Location:2 → 98

PLA2c; Phospholipase A2, a family of secretory and cytosolic enzymes; the latter are either Ca dependent or Ca independent. PLA2 cleaves the sn-2 position of the glycerol backbone of phospholipids (PC or phosphatidylethanolamine), usually in a metal-dependent ...