Lck lymphocyte protein tyrosine kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lckprovided by MGI
Official Full Name: lymphocyte protein tyrosine kinaseprovided by MGI
Primary source: MGI:MGI:96756
See related: Ensembl:ENSMUSG00000000409 AllianceGenome:MGI:96756
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lsk; Lskt; Hck-3; p56Lck; p56<lck>
Summary: Enables several functions, including CD4 receptor binding activity; protein antigen binding activity; and protein phosphatase binding activity. Involved in release of sequestered calcium ion into cytosol. Acts upstream of or within several processes, including positive regulation of macromolecule metabolic process; protein phosphorylation; and regulation of T cell differentiation. Located in cell-cell junction. Colocalizes with plasma membrane. Is expressed in several structures, including 2-cell stage embryo; nervous system; neural ectoderm; sensory organ; and thymus primordium. Human ortholog(s) of this gene implicated in immunodeficiency 22. Orthologous to human LCK (LCK proto-oncogene, Src family tyrosine kinase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in thymus adult (RPKM 430.2), spleen adult (RPKM 43.4) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 4 D2.2; 4 63.26 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (129442142..129467415, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (129548344..129573641, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lymphocyte-Specific Protein Tyrosine Kinase Contributes to Spontaneous Regression of Liver Fibrosis may by Interacting with Suppressor of Cytokine Signaling 1.
Title: Lymphocyte-Specific Protein Tyrosine Kinase Contributes to Spontaneous Regression of Liver Fibrosis may by Interacting with Suppressor of Cytokine Signaling 1.
LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.
Title: LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.
[LAG3 intracytoplasmic acidic environment locally disrupts the association of the Lck kinase to CD4 and CD8, two co-receptors of the TCR].
Title: [LAG3 intracytoplasmic acidic environment locally disrupts the association of the Lck kinase to CD4 and CD8, two co-receptors of the TCR].
LCK-Mediated RIPK3 Activation Controls Double-Positive Thymocyte Proliferation and Restrains Thymic Lymphoma by Regulating the PP2A-ERK Axis.
Title: LCK-Mediated RIPK3 Activation Controls Double-Positive Thymocyte Proliferation and Restrains Thymic Lymphoma by Regulating the PP2A-ERK Axis.
Identification of Potential Biomarkers of Type 2 Diabetes Mellitus-Related Immune Infiltration Using Weighted Gene Coexpression Network Analysis.
Title: Identification of Potential Biomarkers of Type 2 Diabetes Mellitus-Related Immune Infiltration Using Weighted Gene Coexpression Network Analysis.
CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in mice.
Title: CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in mice.
Genetic Loss of LCK Kinase Leads to Acceleration of Chronic Lymphocytic Leukemia.
Title: Genetic Loss of LCK Kinase Leads to Acceleration of Chronic Lymphocytic Leukemia.
Dynamics of the Coreceptor-LCK Interactions during T Cell Development Shape the Self-Reactivity of Peripheral CD4 and CD8 T Cells.
Title: Dynamics of the Coreceptor-LCK Interactions during T Cell Development Shape the Self-Reactivity of Peripheral CD4 and CD8 T Cells.
Lck bound to coreceptor is less active than free Lck.
Title: Lck bound to coreceptor is less active than free Lck.
LCK cooperates with oncogenic FLT3-ITD in cellular transformation
Title: The Src family kinase LCK cooperates with oncogenic FLT3/ITD in cellular transformation.

Submit: New GeneRIF Correction See all GeneRIFs (71)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (3)
Endonuclease-mediated (5) 1 citation
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Lck (e-PCR), detects polymorphism
- UniSTS:526087

M12056 (e-PCR)
- UniSTS:159298

D4Mit203 (e-PCR), detects polymorphism
- UniSTS:116302

X03533 (e-PCR)
- UniSTS:125781

Lck (e-PCR), detects polymorphism
- UniSTS:143761

Lck (e-PCR), detects polymorphism
- UniSTS:143762

PMC166405P2 (e-PCR)
- UniSTS:271589

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATPase binding	ISO Inferred from Sequence Orthology more info
enables CD4 receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables CD4 receptor binding	ISO Inferred from Sequence Orthology more info
enables CD8 receptor binding	ISO Inferred from Sequence Orthology more info
enables SH2 domain binding	ISO Inferred from Sequence Orthology more info
enables SH2 domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables T cell receptor binding	ISO Inferred from Sequence Orthology more info
enables antigen binding	ISO Inferred from Sequence Orthology more info
enables histone H2AXY142 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3Y41 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables non-membrane spanning protein tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables non-membrane spanning protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine kinase activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol 3-kinase binding	ISO Inferred from Sequence Orthology more info
enables phospholipase activator activity	ISO Inferred from Sequence Orthology more info
enables phospholipase binding	ISO Inferred from Sequence Orthology more info
enables phosphotyrosine residue binding	ISO Inferred from Sequence Orthology more info
enables protein antigen binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein tyrosine kinase activity	EXP Inferred from Experiment more info	PubMed
enables protein tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein tyrosine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein tyrosine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in B cell receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within B cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in Fc-gamma receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in T cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in T cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in T cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cell surface receptor protein tyrosine kinase signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of gamma-delta T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intracellular zinc ion homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in peptidyl-tyrosine autophosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of T cell activation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of T cell activation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cysteine-type endopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of gamma-delta T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of leukocyte cell-cell adhesion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of tyrosine phosphorylation of STAT protein	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of uterine smooth muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of T cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of regulatory T cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in release of sequestered calcium ion into cytosol	IDA Inferred from Direct Assay more info	PubMed
involved_in response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
involved_in response to mechanical stimulus	ISO Inferred from Sequence Orthology more info
involved_in response to metal ion	ISO Inferred from Sequence Orthology more info
involved_in response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in response to xenobiotic stimulus	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in endocytic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in immunological synapse	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISS Inferred from Sequence or Structural Similarity more info
located_in pericentriolar material	ISO Inferred from Sequence Orthology more info
located_in pericentriolar material	ISS Inferred from Sequence or Structural Similarity more info
colocalizes_with plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic specialization, intracellular component	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: proto-oncogene tyrosine-protein kinase LCK

Names: leukocyte C-terminal Src kinase; lymphocyte cell-specific protein-tyrosine kinase

NP_001155904.1

EC 2.7.10.2

NP_001155905.1

EC 2.7.10.2

NP_034823.1

EC 2.7.10.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001162432.2 → NP_001155904.1 proto-oncogene tyrosine-protein kinase LCK isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (a).

Source sequence(s)

AL606921

Consensus CDS

CCDS51306.1

UniProtKB/TrEMBL

E9Q696

Related

ENSMUSP00000125777.2, ENSMUST00000167288.8

Conserved Domains (4) summary

cd10362
Location:134 → 234

SH2_Src_Lck; Src homology 2 (SH2) domain in lymphocyte cell kinase (Lck)

cd12005
Location:76 → 129

SH3_Lck; Src homology 3 domain of Lck Protein Tyrosine Kinase

cd05067
Location:248 → 511

PTKc_Lck_Blk; Catalytic domain of the Protein Tyrosine Kinases, Lymphocyte-specific kinase and Blk

pfam07714
Location:256 → 505

Pkinase_Tyr; Protein tyrosine kinase
NM_001162433.2 → NP_001155905.1 proto-oncogene tyrosine-protein kinase LCK isoform b

See identical proteins and their annotated locations for NP_001155905.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b).

Source sequence(s)

AL606921, AL671759

Consensus CDS

CCDS18697.1

UniProtKB/Swiss-Prot

P06240, Q61794, Q61795, Q62320, Q91X65

Related

ENSMUSP00000099656.2, ENSMUST00000102596.8

Conserved Domains (3) summary

cd10362
Location:123 → 223

SH2_Src_Lck; Src homology 2 (SH2) domain in lymphocyte cell kinase (Lck)

cd12005
Location:65 → 118

SH3_Lck; Src homology 3 domain of Lck Protein Tyrosine Kinase

cd05067
Location:237 → 500

PTKc_Lck_Blk; Catalytic domain of the Protein Tyrosine Kinases, Lymphocyte-specific kinase and Blk
NM_010693.4 → NP_034823.1 proto-oncogene tyrosine-protein kinase LCK isoform b

See identical proteins and their annotated locations for NP_034823.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b).

Source sequence(s)

AL606921

Consensus CDS

CCDS18697.1

UniProtKB/Swiss-Prot

P06240, Q61794, Q61795, Q62320, Q91X65

Related

ENSMUSP00000066209.5, ENSMUST00000067240.11

Conserved Domains (3) summary

cd10362
Location:123 → 223

SH2_Src_Lck; Src homology 2 (SH2) domain in lymphocyte cell kinase (Lck)

cd12005
Location:65 → 118

SH3_Lck; Src homology 3 domain of Lck Protein Tyrosine Kinase

cd05067
Location:237 → 500

PTKc_Lck_Blk; Catalytic domain of the Protein Tyrosine Kinases, Lymphocyte-specific kinase and Blk