RMI1 Rmi1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RMI1
Official Full Name: Rmi1p
Primary source: SGD:S000005945
Locus tag: YPL024W
See related: AllianceGenome:SGD:S000005945; FungiDB:YPL024W; VEuPathDB:YPL024W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: NCE4
Summary: Enables enzyme activator activity; four-way junction DNA binding activity; and single-stranded DNA binding activity. Involved in DNA damage response; DNA topological change; and mitotic sister chromatid cohesion. Located in nuclear replication fork. Part of RecQ family helicase-topoisomerase III complex. Used to study primary ovarian insufficiency. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RMI1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (503517..504242)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Optimal Top3/Rmi1 Sumoylation Relies on Sgs1 Interaction, Sgs1 SIMs, and Mms21.
Title: Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates.
The strand-passage activity of Top3-Rmi1 is required for all known functions of Sgs1 in meiotic recombination, including channeling JMs into physiological crossover and noncrossover pathways, and suppression of non-allelic recombination.
Title: Pervasive and essential roles of the Top3-Rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis.
Rmi1 stimulates Top3 in D loop dissolution during homologous recombination.
Title: Top3-Rmi1 dissolve Rad51-mediated D loops by a topoisomerase-based mechanism.
Top3-Rmi1 acts in all meiotic recombination functions previously associated with Sgs1, most notably as an early recombination intermediate chaperone, promoting regulated crossover and noncrossover recombination.
Title: Top3-Rmi1 DNA single-strand decatenase is integral to the formation and resolution of meiotic recombination intermediates.
Genetic analyses revealed that Rmi1 promoted sister chromatid cohesion in a process that was distinct from both the cohesion establishment pathway involving Ctf4, Csm3, and Chl1 and the pathway involving the acetylation of Smc3
Title: Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1.
Rmi1 has a unique regulatory capacity that slows DNA relaxation by Top3 but stimulates DNA decatenation.
Title: Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes.
Study established that Dna2, Sgs1 and RPA constitute a minimal protein complex capable of DNA resection in vitro; in addition it was found that both the Top3 and Rmi1 complex and the Mre11-Rad50-Xrs2 complex have important roles as stimulatory components.
Title: DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2.
A model is proposed in which the Shu proteins act in homologous recombination repair(HRR) to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex.
Title: Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3.
Top3-Rmi1 functions as a complex and suggest that Rmi1 stimulates Top3 by promoting its interaction with ssDNA.
Title: Binding and activation of DNA topoisomerase III by the Rmi1 subunit.
Rmi1 contributes to sister chromatid cohesion.
Title: Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA damage response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA binding	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of RecQ family helicase-topoisomerase III complex	IDA Inferred from Direct Assay more info	PubMed
part_of RecQ family helicase-topoisomerase III complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rmi1p

NP_015301.1

Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase