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    PEP3 tethering complex subunit PEP3 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850840, updated on 9-Dec-2024

    Summary

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    Official Symbol
    PEP3
    Official Full Name
    tethering complex subunit PEP3
    Primary source
    SGD:S000004138
    Locus tag
    YLR148W
    See related
    AllianceGenome:SGD:S000004138; FungiDB:YLR148W; VEuPathDB:YLR148W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    VAM8; VPS18; VPT18
    Summary
    Enables protein-macromolecule adaptor activity. Contributes to phosphatidylinositol binding activity. Involved in several processes, including Golgi to endosome transport; late endosome to vacuole transport; and vacuole fusion, non-autophagic. Located in fungal-type vacuole membrane. Part of CORVET complex and HOPS complex. Orthologous to human VPS18 (VPS18 core subunit of CORVET and HOPS complexes). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See PEP3 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (434641..437397)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene mRNA splicing protein SMD3 Neighboring gene Gid11p Neighboring gene Stm1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. Preston RA, et al. Mol Cell Biol, 1991 Dec. PMID 1944264, Free PMC Article
    2. Nystatin effects on vacuolar function in Saccharomyces cerevisiae. Bhuiyan MS, et al. Biosci Biotechnol Biochem, 1999 Jun. PMID 10427694
    3. A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. Robinson JS, et al. Mol Cell Biol, 1991 Dec. PMID 1840635, Free PMC Article
    4. PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress. Ding J, et al. Appl Microbiol Biotechnol, 2015 Oct. PMID 26051671, Free PMC Article
    5. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. Mu FT, et al. J Biol Chem, 1995 Jun 2. PMID 7768953

    See all (128) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. beta-propeller of Vps18 is required for HOPS stability and function and that it can serve as a starting point for further structural analyses of the HOPS tethering complex.
      Title: Structural identification of the Vps18 β-propeller reveals a critical role in the HOPS complex stability and function.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to phosphatidylinositol binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-macromolecule adaptor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Golgi to endosome transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in endosome organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in late endosome to vacuole transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in organelle fusion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of SNARE complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vacuole fusion, non-autophagic IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vacuole fusion, non-autophagic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vacuole organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in vesicle docking involved in exocytosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in vesicle docking involved in exocytosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vesicle tethering IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vesicle-mediated transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of CORVET complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of HOPS complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of HOPS complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in bounding membrane of organelle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in fungal-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuolar membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tethering complex subunit PEP3
    NP_013249.1
    • Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      434641..437397
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182035.1 → NP_013249.1  TPA: tethering complex subunit PEP3 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013249.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYE3, P27801
      UniProtKB/TrEMBL
      A7A147, B3LTA9, B5VN77, C7GMK0, C8ZDB8, N1NYA7
      Conserved Domains (3) summary
      smart00299
      Location:585 → 737
      CLH; Clathrin heavy chain repeat homology
      cd16462
      Location:824 → 899
      RING-H2_Pep3p_like; RING finger, H2 subclass, found in Saccharomyces cerevisiae vacuolar membrane protein PEP3 (Pep3p) and similar proteins
      pfam05131
      Location:214 → 378
      Pep3_Vps18; Pep3/Vps18/deep orange family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P27801.1

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